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Amazon Shop for: ½ Pint Jars, Rye Grain, Ultrasonic Humidifier, Wild Bird Seed

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Invisiblegema
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Registered: 10/24/04
Posts: 1,767
Loc: t(here)
Inoculating Jars
    #3899809 - 03/10/05 09:55 PM (11 years, 8 months ago)

After a successful 1st attempt, I'm having a hell of a time repeating my success. I can't get past the green mold in my rye grain jars in the incubating stage. I inoculated 16 jars and ALL got contams! I do use a pc and painstakenly follow the tek to a T. I get my spores from a sponser but they did smell like cigarette smoke. I kind of narrowed down my problem to me inculating my jars.

I'm trying to inculate my jars in a clean environment and tried the kitchen with poor results. I can't afford a humidifier right now to keep the dust down but was thinking about knocking up my jars in the bathroom after i take a shower. anybody having the same problems as me and have any ideas? sorry for my rant.


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OfflineSuntzu
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Registered: 10/15/99
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Re: Inoculating Jars [Re: gema]
    #3899978 - 03/10/05 10:41 PM (11 years, 8 months ago)

When you're having these kinds of problems, it's a good idea to keep one jar aside, uninoculated, as a 'negative control'. If it remains clean, it helps narrow down the source.
As for the humidifier helping with contams, I'm not so sure about that. . .in the short term, heavy misting/spraying does reduce airborne particles, but a humidified 'dusty' room actually aggravates the problem. Mold spores, etc. can germinate when it's moist; you're much better off getting even a cheap air filter. I'd be very wary of trying to humidify a room to combat contamination.
Another thing to consider is experimenting with different grain. Some people have notorious problems with rye [I've NEVER gotten it to work] and find birdseed much easier. . .and vice versa.
A simple glovebox is cheap and easy to make, and can make all the difference as well. Consider whether the filter system on the jars is adequate, and if it could be made better.
If you tackle it on all these fronts, you will succeed.


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OfflinePhotoguy
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Registered: 02/24/05
Posts: 553
Loc: Drunk in Texas
Last seen: 11 years, 7 days
Re: Inoculating Jars [Re: Suntzu]
    #3900175 - 03/10/05 11:24 PM (11 years, 8 months ago)

Great idea as keeping one jar aside. Goddamn thats really a great idea, we should spread the word on that one. If i new how to give a rating, i would for that. I am going to do that every time now. Thanks.


--------------------
You gotta check yo' self before you wreck yo' self

B, Z, F, Burma, Cambodian, Golden Teacher, Puerto Rico, South American, Costa Rica, African

My name is Ender Wiggen and I will own you soon. Bean is my second in command, he is PC'ing jars right now.


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Offlinetarget022
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Registered: 03/02/05
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Re: Inoculating Jars [Re: Photoguy]
    #3900616 - 03/11/05 01:10 AM (11 years, 8 months ago)

I'm having the same problem. My rye grain starts out looking good then just goes green. I now know its not the grain because I have a negative control(right jar).

The plates I use to inoculate don't have any green on them but its the only place the mold could have come from. Is that possible?

The right plate seems furrier than the left.
I started the plate with a piece of a sawdust block, but I would like to start a plate from spores, but have had no luck, they don't seem to want to grow. Any suggestions?

Sal


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