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Amazon Shop: ½ Pint Jars, Vermiculite

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Offlinedsd2o01
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keeping jars sterile after inoculation
    #3897039 - 03/10/05 12:05 PM (12 years, 3 months ago)

I am now waiting for the mycelium to form. A friend of mine said that the inoculation holes in the jar lids will allow contaminates to get in and ruin the substrate. Should i cover the jars with anything or are they going to be fine as is?

Thanks.


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OfflinePhotoguy
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Registered: 02/24/05
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Re: keeping jars sterile after inoculation [Re: dsd2o01]
    #3897049 - 03/10/05 12:08 PM (12 years, 3 months ago)

Are they in a incubating chamber and is said chamber clean or is it on your living room floor next to the week old pizza box?


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You gotta check yo' self before you wreck yo' self

B, Z, F, Burma, Cambodian, Golden Teacher, Puerto Rico, South American, Costa Rica, African

My name is Ender Wiggen and I will own you soon. Bean is my second in command, he is PC'ing jars right now.


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InvisibleHolydiver
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Re: keeping jars sterile after inoculation [Re: dsd2o01]
    #3897054 - 03/10/05 12:09 PM (12 years, 3 months ago)

If you followed a specific tek and included the dry verm barrier, either just a verm barrier, or foil crater + verm (ramsey seal) then you just leave the jar as is and contams will not enter the jar if done correctly. Leave your holes open to breathe.


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To find a place to live between the negatives and positives.


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Offlinedsd2o01
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Re: keeping jars sterile after inoculation [Re: Holydiver]
    #3897060 - 03/10/05 12:11 PM (12 years, 3 months ago)

Thanks guys. They are not in an incubator, but they do have the 1/2 inch layer of dry vermiculite at the top of the jar. I am storing them in a drawer that is rarely opened.


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OfflineMages
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Re: keeping jars sterile after inoculation [Re: dsd2o01]
    #3897085 - 03/10/05 12:19 PM (12 years, 3 months ago)

You should be fine. A very few bad things might get in through the holes, BUT microbes aren't bugs; they can't crawl down through the verm. barrier into the goodies. They either have to grow where they lie or wait for wind or water to carry them somewhere else. That's why P.F. style cakes are so good for beginners.


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- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh


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OfflinePhotoguy
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Re: keeping jars sterile after inoculation [Re: Mages]
    #3897095 - 03/10/05 12:21 PM (12 years, 3 months ago)

BTW whats the temp in the drawer? Do you have a way a checking that out. Temps pretty important during this stage, and we all here in the Shroomery want every person to succeed in their endevors.


--------------------
You gotta check yo' self before you wreck yo' self

B, Z, F, Burma, Cambodian, Golden Teacher, Puerto Rico, South American, Costa Rica, African

My name is Ender Wiggen and I will own you soon. Bean is my second in command, he is PC'ing jars right now.


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OfflineMushroomFriend
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Re: keeping jars sterile after inoculation [Re: dsd2o01]
    #3897103 - 03/10/05 12:23 PM (12 years, 3 months ago)

I cover the lid, not tight but loose, with a piece of alufoil. Hardly ever any contams.


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Offlinedsd2o01
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Re: keeping jars sterile after inoculation [Re: dsd2o01]
    #3899084 - 03/10/05 07:26 PM (12 years, 3 months ago)

i do not know what temperature it is inside the drawer. The only thing about temperature in the guide was about inside the terrarium being in the upper 70's. Is there a certain temperature that should be met during the culturizing stage?


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Amazon Shop: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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