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Amazon Shop for: ½ Pint Jars, Agar

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Offlinelaughingbuddha
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Registered: 10/19/04
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how long is too long?
    #3890669 - 03/09/05 02:40 AM (11 years, 8 months ago)

how long does one keep a liquid culture of B+ in the incubator? this is the third week @ 86F; it's been showing solid growth for two weeks. i'm not quite ready to use it yet. would it be better transfered to the fridge until i'm ready to use it, or should i just leave it?

it's incubating with some newly prepared liquid cultures of south african and panaeolus cyanescens that are both already showing white floaties (awesome!) the incubator has a substantial amount of humidity inside, incidentally, perhaps because the jars are covered only with a few sqaures of tyvek, rubber-banded around the jar. should i take the B+ out? is it a problem it's so humid?


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OfflineShroomGuy86
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- [Re: laughingbuddha]
    #3890676 - 03/09/05 02:42 AM (11 years, 8 months ago)

-


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Offlinelaughingbuddha
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Re: how long is too long? [Re: ShroomGuy86]
    #3890686 - 03/09/05 02:44 AM (11 years, 8 months ago)

awesome! good luck brah.


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"The more I read, the more I meditate; and the more I meditate, the more certain I am that I know nothing." -Voltaire


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OfflineShroomGuy86
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- [Re: laughingbuddha]
    #3890695 - 03/09/05 02:47 AM (11 years, 8 months ago)

-


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Invisibleagar
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Re: how long is too long? [Re: laughingbuddha]
    #3890941 - 03/09/05 03:29 AM (11 years, 8 months ago)

Once you have good thick growth in an LC, it is best to stall it ASAP, via the fridge.


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Offlinelaughingbuddha
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Re: how long is too long? [Re: agar]
    #3896713 - 03/10/05 09:24 AM (11 years, 8 months ago)

how come agar?


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OfflineCray
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Re: how long is too long? [Re: laughingbuddha]
    #3896868 - 03/10/05 10:47 AM (11 years, 8 months ago)

I think, it's because it'll start to dry out, and bruze and/or get contam.


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Offlinelaughingbuddha
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Re: how long is too long? [Re: Cray]
    #3897720 - 03/10/05 02:50 PM (11 years, 8 months ago)

how does a liquid culture dry out? how does a liquid culture "bruze" ?(c'mon dude, it's bruise) neither of those scenarios make sense with a liquid culture.


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OfflineXTCollection
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Re: how long is too long? [Re: laughingbuddha]
    #3900084 - 03/10/05 10:59 PM (11 years, 8 months ago)

Or the mycelium could switch what I call like a growing state, were it produces compounds but doesnt fruit. I think using that to start more jars might be bad... Not sure tho, Im sure some good guy like roadkill or agar will say for sure.

But it only makes sense too, preserves the culture.


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OfflineSHROOMpimp
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Re: how long is too long? [Re: XTCollection]
    #3900147 - 03/10/05 11:17 PM (11 years, 8 months ago)

if you a leave the jars "growing" for too long the myc. will use up the nutrients in the sugars. i'm not sure about the other scenarios though. agar will probably chime in again and give us the full scoop.

SHROOMpimp


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Offlinenambi
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Re: how long is too long? [Re: SHROOMpimp]
    #3985031 - 03/29/05 02:01 PM (11 years, 8 months ago)

SO how long will it last in the fridge?


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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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