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Amazon Shop: ½ Pint Jars, Agar

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OfflineJoops
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two weeks with no karo growth
    #3876075 - 03/06/05 05:23 AM (12 years, 9 months ago)

I made a karo jar two weeks ago and shot spores in to it. It still has no growth. A week ago I made a new syringe and 3 more karo jars, and they too show no growth. Two weeks is beginning to concern me. I see all the black spores swirling around but thats all.

Any thoughts? Something should be colonizing, whether it be myc or contam'd myc... I did not use too much karo, and I pc'd for about 15-20min. Very strange!


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InvisibleRoadkillM
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Re: two weeks with no karo growth [Re: Joops]
    #3876217 - 03/06/05 06:31 AM (12 years, 9 months ago)

how much karo did you use?

how much water did you use?

need the ratios...to help.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleTaskenti
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Re: two weeks with no karo growth [Re: Roadkill]
    #3876392 - 03/06/05 09:37 AM (12 years, 9 months ago)

well my friend said he was having this problem too, and he was using 25 ML of karo with 500 ML water. Any suggestions for him roadkill?


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CIVIC IS Mycoshack!! Be careful trading with him!!
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InvisibleHolydiver
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Re: two weeks with no karo growth *DELETED* [Re: Taskenti]
    #3876414 - 03/06/05 09:57 AM (12 years, 9 months ago)

Post deleted by Diver

Reason for deletion: Corrected, see below.



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To find a place to live between the negatives and positives.


Edited by Diver (03/06/05 10:16 AM)


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InvisibleTaskenti
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Re: two weeks with no karo growth [Re: Holydiver]
    #3876442 - 03/06/05 10:13 AM (12 years, 9 months ago)

LOL. He said he thought the tek called for tsp and thats why he used 5 ML per 100 ML of water. So its supposed to be 15 ML per 100 ML...lmao imma call him up and tell his dumb ass! Thanks diver.


--------------------
CIVIC IS Mycoshack!! Be careful trading with him!!
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InvisibleHolydiver
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Re: two weeks with no karo growth [Re: Taskenti]
    #3876450 - 03/06/05 10:15 AM (12 years, 9 months ago)

It is teaspoon, typing error on my part. Sorry about that.

I teasponn is 5 ML, general working mixture is 5 ML karo/honey water to 100 ML water.

So, your friends mixture of 25 ML Karo to 500 ML water should have worked.


--------------------
To find a place to live between the negatives and positives.


Edited by Diver (03/06/05 10:23 AM)


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InvisibleArp
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Re: two weeks with no karo growth [Re: Holydiver]
    #3876499 - 03/06/05 10:57 AM (12 years, 9 months ago)

Did you try bottled/distilled water?

I did have very poor (none) success trying malt, dextrose, honeys & syrups using tap water.

I left it for agar work and with my first attempts I used the tap-water and the growth was very slow. Switching to distilled solved it :-P


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Offlinebrewwhaha1
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Re: two weeks with no karo growth [Re: Holydiver]
    #3876508 - 03/06/05 11:02 AM (12 years, 9 months ago)

I like this simple method for measureing. I imagine karo would be the same as honey (i use honey). Let water boil for 10 min. pour into 500ml jar 3/4 full. use a teaspoon and put it in the hot water. now dunk the hot spoon into the honey. don't scoop it out--lift it straight up. this is the amount of honey i use for a pint jar. Dunk the spoon into the pint jar with hot water give it a stir. let it cool to room temp and innoc.
Not very scientific but it works for me. i use a finishing nail size hole in my metal lid and use tinfoil wrapped over the top to keep contams out. incubate at 82F. For whatever reason i don't have problems with contams. Perhaps i'm just lucky. if you have problems with persistant contamination you might have to try a more sanitized approach.


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OfflineTweexican
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Re: two weeks with no karo growth [Re: brewwhaha1]
    #3876546 - 03/06/05 11:21 AM (12 years, 9 months ago)

you know, im fed up with karo. its one of those procedures that ends up either going perfectly or fucking over 30 jars at a time. I hate the stuff. I'm sticking with agar from now on...


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Offlinelaughingbuddha
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Re: two weeks with no karo growth [Re: brewwhaha1]
    #3876565 - 03/06/05 11:31 AM (12 years, 9 months ago)

no. not necessary, any of that. microwave 1 quart jar 2/3 full of distilled water & about 1 tbl of honey for 6 minutes. wrap the lid with a few squares of tyvek held in place with rubber bands. when done, cool in fridge. once cool, innoc, wait a few days/1 week. if you've waited 2 weeks and you don't see any little white floaties or whatnot something went wrong. mayhap you innoc'd while the karo was still hot? you might try boiling or microwaving--it isn't always necessary to pressure cook, despite what the neat freaks on here might say. so as for your karo jars, if it's 2 weeks, and 0 growth: bummer. try again, cause they're never gonna show growth. good luck brah!


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"The more I read, the more I meditate; and the more I meditate, the more certain I am that I know nothing." -Voltaire


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InvisibleHolydiver
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Re: two weeks with no karo growth [Re: laughingbuddha]
    #3876625 - 03/06/05 11:51 AM (12 years, 9 months ago)

I'm not a fan of Karo either, it caramelizes in the PC and you end up with all sorts of floaters and inconsistencies. I'd take honey any day for liquid culture over Karo.


--------------------
To find a place to live between the negatives and positives.


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InvisibleRoadkillM
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Re: two weeks with no karo growth [Re: Taskenti]
    #3876857 - 03/06/05 01:00 PM (12 years, 9 months ago)

not sure bruce.

I have been having great success with Karo...recently.
I used to only use honey.
Read a post from Psilocybeingzz on Karo...he was having good results and asked him for his ratio's.
Looked around the house...found a few bottles of Karo sitting in my pantry.

Ratio's he gave me and that I have been using :

Karo Tall 1/2 pint jar :
4cc's of Karo (light corn syrup)
96cc's of Warm Water

Karo Pint jar :
8cc's of Karo (light corn syrup)
192cc's of Warm Water

larger size jars double the recipy.

I add a marble to the jar...to break up the mycelium to make making syringes easier.
I pc for 30 minutes at 15 psi.

side note...I haven't had any type of caramelization problems.



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineJoops
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Re: two weeks with no karo growth [Re: Roadkill]
    #3877529 - 03/06/05 04:05 PM (12 years, 9 months ago)

I heard 1 table spoon per 100ml water. I might have had slightly more karo per water but not much, perhaps an extra table spoon for a 3/4 full pint jar. No carmalization, and jars were completely cooled overnight before inoculation. I'll look in to honey and try to find what kind of honey is needed and try again with honey.


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OfflineXTCollection
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Re: two weeks with no karo growth [Re: Roadkill]
    #3877552 - 03/06/05 04:08 PM (12 years, 9 months ago)

AFOAF tried several light karo jars a day or 2 ago. He used roadkills as a baseline, and did minor to major adjustments with heavey to light syrup/water ratios.

Ill post back for him w/ pics. If only 2-3 jars go, then the ratios dont have much room for adjusting. But if most go, then its something else, maybe tap or distilled water stuff.


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Amazon Shop: ½ Pint Jars, Agar

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