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Amazon Shop for: ½ Pint Jars, HEPA Filter, Mycobags

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OfflineSucellos
Stranger
Registered: 01/31/05
Posts: 275
Last seen: 9 years, 8 months
WTF How Did This Happen?
    #3872625 - 03/05/05 01:12 PM (11 years, 8 months ago)

Alright so I knocked up 4 mycobags about 10 days ago and all were colonizing fine. My friend and I were going crazy that it was actually going fine. So today I go to check them and two of the bags with the most colonization had this fuzzy dark green stuff mixed in with the mycellium. I can't figure out how this happened. I swabbed the inoculation sites with alcohol and flame sterilised the needles before inoculation other than that I have no idea how contams would have gotten in. They are being incuabated in a crawl space but there is a hepa filter patch on the mycobags and that would keep out all the shit. There was accumulations of water in the bottom of all of them but not puddles. Fuck. Can I do anything here? I am goiong to just let them go and see if the mycellium eats the mold but how did this happen?


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
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Re: WTF How Did This Happen? [Re: Sucellos]
    #3872640 - 03/05/05 01:18 PM (11 years, 8 months ago)

Contams can still get in. The mycelium WONT eat the mold. WONT happen. I keep jars all the time before tossing..they may fruit in the bags but if you ate something like this itd make you sick...this green/white in the jar looked like this for a month before this picture and a month later you can see the green is still there.


Its hard to say for sure. Perhaps didnt PC for to long? Bags easily bellow air in and out any time you touch them. If you didnt inject in a sterile enough spot and the bags moved alot when injecting, or even after injecting in the spot. The filter patch isnt exactly a HEPA filter..just a filter...

Some contams are normal too...it wouldnt be a shcok for one or two bags to contam. Sucks you only have four to begin with though....toss those two bags though or your putting your others, and the whole grow operation at risk. Contams arent worth it!


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleHolydiver
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Registered: 03/20/01
Posts: 5,155
Loc: The midnight sea
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Re: WTF How Did This Happen? [Re: Sucellos]
    #3872642 - 03/05/05 01:18 PM (11 years, 8 months ago)

It's a losing battle, sorry but you'll need to toss those bags A.S.A.P. Did you obtain the syringe from a vendor, or make it yourself? If you got presealed myco bags the source of contamination is either from your syringe or in a lack of sterile practices during inoculation or lastly contams got in the bag itself somehow.


--------------------
To find a place to live between the negatives and positives.


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Offlinelemon_lw
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Re: WTF How Did This Happen? [Re: Holydiver]
    #3872654 - 03/05/05 01:24 PM (11 years, 8 months ago)

my guess is the filter on the bag sucks ass. the contams didnt show up untill 10 days after inoc. in my experience contams will show signs of growth before mycelium has a chance to. so yeah learn about the PF TEk and youll be happy.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineMontyRev
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Registered: 01/22/05
Posts: 96
Last seen: 10 years, 8 months
Re: WTF How Did This Happen? [Re: lemon_lw]
    #3872734 - 03/05/05 02:01 PM (11 years, 8 months ago)

what? i thought bags from mycobag.com were already already pressure cooked and cleaned prior to you buying them...
so if thats the case it should be the syringe


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InvisibleHolydiver
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Re: WTF How Did This Happen? [Re: MontyRev]
    #3872772 - 03/05/05 02:12 PM (11 years, 8 months ago)

Quote:

MontyRev said:
what? i thought bags from mycobag.com were already already pressure cooked and cleaned prior to you buying them...
so if thats the case it should be the syringe




They are, but filter patches aren't 100% protection from contams; especially if they get wet. Anything is possible, so is contamination of a pre-sterilized MycoBag.


--------------------
To find a place to live between the negatives and positives.


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Offlinescatmanrav
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Registered: 05/08/04
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Last seen: 3 years, 10 months
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Re: WTF How Did This Happen? [Re: Holydiver]
    #3872795 - 03/05/05 02:16 PM (11 years, 8 months ago)

If they all contam, the syringe is a possibility. If two make it, its not the syringe.

Since it started long after the myc I would bet 99% that its not the syringe, even if all bags get lost. I do jars all the time. Tons. My first bags..lost 10 of 10 to contams. Bags are a little difficult to work with is all. Gotta be carefull with them.

By the way, mycobags come with persterilized with substrate and empty and kinda sterile, but not really. Wasnt sure if you made them yourself. My guess is..handling of the bags when, and after, injecting.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
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Posts: 6,228
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Re: WTF How Did This Happen? [Re: Sucellos]
    #3872823 - 03/05/05 02:24 PM (11 years, 8 months ago)

How does it happen? Faulty sterile culture technique, not always as simple as some might think! I've seen inexperienced growers often recontaminate inadvertantly because of there faulty technique which includes every object involved not just the substrate. GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinefallingaway24
catatonic selfmedicator
Registered: 12/25/04
Posts: 419
Last seen: 1 year, 10 months
Re: WTF How Did This Happen? [Re: hyphae]
    #3873492 - 03/05/05 05:51 PM (11 years, 8 months ago)

i had the same prob with mycobags, i had 2 contam when the bag was like 25% done, i emailed them and told them that out of 6 bags 2 got contamed, and i used the same techniqe for all of them and the same syringe, but anyways they where happy to send me 2 new ones.


--------------------
"Most people don't know how they're gonna feel from one moment to the next. But a dope fiend has a pretty good idea. All you gotta do is look at the labels on the bottles." ~drugstore cowboy~


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Amazon Shop for: ½ Pint Jars, HEPA Filter, Mycobags

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