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Offlinek2nride
journeyman
Registered: 04/19/04
Posts: 85
Last seen: 12 years, 21 days
why dont my jars colonize?
    #3864133 - 03/03/05 08:12 PM (12 years, 5 months ago)

This is my second syringe ive gone through without luck. I tried huautla my first time and got 5 jars of colonized substrate. The jars didnt have any problems at all. I used 1/4 cup brf, 1/4 cup water, and 1/2 cup vermiculite. They were colonized in about 1 1/2 weeks. My second try ended up without any colonization at all. No spores even germinated after 3 weeks. Now I have this golden teacher syringe that I just ran out of. I have 5 jars. All of them but 1 has spots of mycelium. They all were growing good until they all just stopped. I had one part colonized with rhy. growth on one side and the other was just the opposite. I know that this means the substrate is too dry, but everything is the same as it was with the huautla. I opened one of the jars and it seemed like the substrate was not dry at all. even where it had stopped growing. this pisses me off because i have wasted $40 on damn syringes and i still dont have any mushies. i have a few questions because its not clear about steralization because every tek is different. When I steam steralize them (i dont have a pc), are the lids supposed to be on tight? loose? do the holes have to be covered during steralization? how high am i supposed to have the burner on? Ill put it on med-high until it startes to boil then ill turn it down to med-low to simmer for an hour. Since my jars are pretty much fucked? can I take out the colonized parts of each jar and combine them somehow to get another jar going?


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: why dont my jars colonize? [Re: k2nride]
    #3864222 - 03/03/05 08:29 PM (12 years, 5 months ago)

Quote:

lids supposed to be on tight?



Don't really crank on the lids. Just turn the bands until they seat.

Quote:

holes have to be covered during steralization?




Cover the holes with tape during sterilization. Remove the tape after colonization is well underway - this will speed things up a bit.

Quote:

my jars are pretty much fucked?



Those jars that you opened are definitely goners. The others may still colonize. Remove the tape from the holes and wait.

Quote:

can I take out the colonized parts of each jar and combine them somehow to get another jar going?




If you had a completely sterile place to work that might be a possibilty. The odds of it being successful are very small.

Try using this Tek next time. Follow it exactly and you should be fine.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinek2nride
journeyman
Registered: 04/19/04
Posts: 85
Last seen: 12 years, 21 days
Re: why dont my jars colonize? [Re: dog]
    #3864295 - 03/03/05 08:41 PM (12 years, 5 months ago)

Well i believe that diff. parts of the united states have less chance of gettin contaminated. I've never been really careful. I have even made 2 casings with 1/2 pint cakes that i didnt steralize at all and they didnt contanimate. Thats why I dont think that opening my jars will make them go bad. They might, but im not very clean with mushroom cultivation at all and ive never gotten any kind of contamination. Oh well we will see. Thanks for the info. Btw I also wanna add that I took out a chunk of mycelium and made a mycelium water syringe and squirted some of it into this unsteralized drinking glass with some substrate mixture. the mycelium is growing good. i filled the cup up with some "UNSTERALIZED" substrate. we'll see if it works or not.


Edited by k2nride (03/03/05 08:43 PM)


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OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 27 days, 3 hours
Re: why dont my jars colonize? [Re: k2nride]
    #3864425 - 03/03/05 09:01 PM (12 years, 5 months ago)

You might not have gotten mold, but I'd be willing to bet the bacteria got ya! If you don't sterilize your jars, you've got a better chance of winning the lottery 5 times than you do having success in this hobby.


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 7 months
Trusted Cultivator
Re: why dont my jars colonize? [Re: Hotnuts]
    #3865844 - 03/04/05 02:11 AM (12 years, 5 months ago)

Some people need to learn the long and hard way.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleFloydseamus
Fun Guy
Male User Gallery

Registered: 05/20/02
Posts: 1,951
Loc: Long Island Flag
Re: why dont my jars colonize? [Re: k2nride]
    #3866543 - 03/04/05 05:46 AM (12 years, 5 months ago)

In my opinion, when i was doing brf jars, the best thing to do as far as the burner intensity, is to crank that bitch up almost all the way.... The steam in the pot should inhibit drying of the substrate... Like my brother up there said, follow the teks exactly..... To the letter.... Once you have a few successes under your belt, then do your own thing.... The first time I did BRF jars, or any mushie growing for that matter, i followed the tek exactly, with treasure coast from sporeworks, and had 100% success..... I don't really know without more info, and really picking it apart with you, what went/is going wrong, but i do know that you will get it, don't give up... And most of all.... good luck brother....


--------------------


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