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azurescens
member
Registered: 02/17/04
Posts: 717
Last seen: 15 years, 8 months
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Growth after freezing!!!!
#3741933 - 02/06/05 06:24 PM (19 years, 20 days ago) |
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I posted a few weeks ago asking if myc would survive freezing temps. After receiveing mixed responses at best, I decided to do my own experiment. 4 agar plate with good growth were placed into a freezer for 1,2,3,4 days respectively. Upon taking out and placing back into environment of temps 0f 82-86....ALL 4 PLATES resumed growth and are still growing healthily. Hope this answers some questions concerning this matter. Strains were
plate 1------Equador 2------------KSS 3------------KSS 4------------KSS
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kronnyQ
SuperstudExtraordinaire


Registered: 07/22/04
Posts: 2,488
Loc: Anytown USA
Last seen: 1 year, 5 months
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Re: Growth after freezing!!!! [Re: azurescens]
#3741994 - 02/06/05 06:35 PM (19 years, 20 days ago) |
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Interesting....any plans to test myc frozen for long periods of time e.g. weeks, months?
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MushroomFriend
Vargen ska fanleva!!


Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 2 days
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Re: Growth after freezing!!!! [Re: azurescens]
#3741998 - 02/06/05 06:36 PM (19 years, 20 days ago) |
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Thanks for testing! How cold did they get?
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azurescens
member
Registered: 02/17/04
Posts: 717
Last seen: 15 years, 8 months
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Just as cold as my freezer which is below 32 whereas the water freezes but I idnt/dont have a thermometer. And yes, I do have plans for future exp regarding longer periods of exposed time. Although I must admit, it makes me sad to think of wasting good cultures of such sweet myc.
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azurescens
member
Registered: 02/17/04
Posts: 717
Last seen: 15 years, 8 months
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Re: Growth after freezing!!!! [Re: azurescens]
#3742941 - 02/06/05 09:58 PM (19 years, 20 days ago) |
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 8 days, 4 hours
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Re: Growth after freezing!!!! [Re: azurescens]
#3861492 - 03/03/05 08:05 AM (18 years, 11 months ago) |
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Interesting....
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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: Growth after freezing!!!! [Re: azurescens]
#3861528 - 03/03/05 08:20 AM (18 years, 11 months ago) |
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I remeber that post , ANNO was also doing some basic research on it. Oh no that was with syringes. Ya know this draws another question . I wonder if during the growth of a culture...if one developed some sort of contam growth on the petri ...if freezing it for a few days might kill off the invader leaving behind the myc. Many if not most bacterial invaders can be killed off by freezing them. HUM..... wonder what freezing a casing that has seen some nasties might work hmmmmmmmmm much interesting angles to approach possible ways to kill off contams. Keep us posted to your findings plz! GL
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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kronnyQ
SuperstudExtraordinaire


Registered: 07/22/04
Posts: 2,488
Loc: Anytown USA
Last seen: 1 year, 5 months
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Re: Growth after freezing!!!! [Re: tripndicular]
#3861581 - 03/03/05 08:41 AM (18 years, 11 months ago) |
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You know, thats a good idea.
I have some casings that I know are infected with Bacilus and cobweb....putting one in the freezer right now.
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