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Shop:   Original Seeds Store Buy CBD, Compare CBD   Amazon ½ Pint Jars, Agar, Malt Extract, Petri Dish, Pressure Cooker, Scales

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InvisibleNDK
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Registered: 07/13/01
Posts: 186
Malt Agar Recipes
    #385310 - 09/04/01 08:37 AM (15 years, 8 months ago)

Hi

I want to get into agar. I have some liquid malt extract and some Clearwater Agar Agar flakes - both from a health store.

Will this do for starters?
What proportions should I use? Ideally I'd like the liquid malt amount in volume and not weight as I don't have accurate scales. I can do the agar by weight by dividing up the bag proportionally. I hope.

I have read a few articles on germinating a spore print. Some say mix with water first some say use it dry. What's the story there?

Thanks




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Anonymous

Re: Malt Agar Recipes [Re: NDK]
    #385873 - 09/04/01 11:08 PM (15 years, 8 months ago)

20 grams of agar agar, go back to the healthfood store and use there scales, break up into 10 or 20 gram individual packs.
2 wopping tablespoons of malt syrup
1000 ml or 1 liter of water
heat water in pot on stove add malt syrup, stir until it dissolves, add agar agar, continue to stir until dissolved.
Pour into (2) 1 quart jars, replace inverted lid on jars, screw tight, losen a little bit so it is not sealed. Cover with tinfoil.
Pressure cook at 15 psi for at least 20 minutes to be safe, make sure you monitor your pressure cooker to reduce heat without to many shakes of the weight, otherwise your liquid will boil over. And if it gets to hot your Malt will caramelize.
Each jar will have approx. 500 ml worth of MEA. Pour plates in a very clean box or whatever you use.
I have yet to have to rehydrate spores from a print!!!
Do a little research before you start.




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OfflineAzure
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Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 15 years, 23 days
Re: Malt Agar Recipes [Re: ]
    #385980 - 09/05/01 01:39 AM (15 years, 8 months ago)

If you melt the agar and malt together and then pour them into petri dishes prior to sterilization, you minimize contamination.



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Anonymous

Re: Malt Agar Recipes [Re: Azure]
    #386220 - 09/05/01 12:11 PM (15 years, 8 months ago)

I use plastic petris, so I don't pressure cook them!!!



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Offlinesylo
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Registered: 10/30/00
Posts: 219
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Re: Malt Agar Recipes [Re: NDK]
    #386749 - 09/06/01 03:03 AM (15 years, 8 months ago)

It is hard to prepare agar on a stove top. The agar settles to the bottom and burns if you don't stand and stir and stir and the mixture foams and boils over in a flash. Preparing agar in a microwave is much easier.

Spores will hydrate on agar so there is no need to pre-hydrate them.



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OfflineDinoMyc
Ipsa scientiapotestas est
Registered: 11/14/99
Posts: 1,080
Last seen: 10 years, 11 months
Re: Malt Agar Recipes [Re: ]
    #386951 - 09/06/01 12:21 PM (15 years, 8 months ago)

you can pressure cook plastic pitri dishes.....

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InvisibleUna
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Registered: 03/01/01
Posts: 970
Re: Malt Agar Recipes [Re: DinoMyc]
    #386988 - 09/06/01 01:00 PM (15 years, 8 months ago)

In reply to:

you can pressure cook plastic pitri dishes.....




and you will end up with a clump of plastic....

Need spores? We got spores!
Visit MUSHMUSH
for the highest quality exotic mushroom spores.
info@mushmush.nl


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Offlineblackout
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Registered: 07/17/00
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Re: Malt Agar Recipes [Re: Una]
    #387035 - 09/06/01 02:25 PM (15 years, 8 months ago)

depends what polymer it is



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