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Anonymous

Where Spores Are Deposited
    #3852103 - 03/01/05 02:58 PM (11 years, 9 months ago)

Hey guys

Just PC'ed 12 PF jars a few days ago, waited a day to let them cool. Now when I went to inoculate them with some GT spores, I was having trouble injecting the spore solution into the side of the jar so that it could drip down the side. Instead, it seemed to go more into the center of the cake, about 1/2 cm from the edge. Each jar had 4 holes that I injected into.

Is this a problem? Will my jars simply take longer to colonize?

Thanks!


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OfflineCiv
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Re: Where Spores Are Deposited [Re: ]
    #3852134 - 03/01/05 03:04 PM (11 years, 9 months ago)

Quote:

Is this a problem? Will my jars simply take longer to colonize?




It wont be a problem, it will just take more time. The more your spores are spread out during the innoc the more area you cover. Well when useing PF cakes.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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Anonymous

Re: Where Spores Are Deposited [Re: Civ]
    #3852182 - 03/01/05 03:13 PM (11 years, 9 months ago)

Great, thanks. =0)

I think I'm going to go ahead and shake them to help spread out the spores.


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OfflineReject
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Re: Where Spores Are Deposited [Re: ]
    #3852190 - 03/01/05 03:15 PM (11 years, 9 months ago)

don't shake pf jars


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OfflineHambo
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Re: Where Spores Are Deposited [Re: ]
    #3852196 - 03/01/05 03:17 PM (11 years, 9 months ago)

It was awkward for me using the PF tek first time, the needles were not quite long enough. The verm would probably absorb the spore water pretty much, shaking won't do much to spread it around i reckon.

When I make PF jars now, I just use tyvek filter and no top verm layer, have 2 innoc points, stick the needle in and spray a bit of sporewater at the sides of the jar in a semi-circular motion, then cover the holes as the needle comes out with a bit of sticky plaster. This seems to work pretty well.


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InvisibleNeedMoreSleep
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Re: Where Spores Are Deposited [Re: ]
    #3852206 - 03/01/05 03:19 PM (11 years, 9 months ago)

Quote:

DirtyHippie said:
I think I'm going to go ahead and shake them to help spread out the spores.




Only accomplishment doing this =====> :stonedjerk:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Offlinethenewguy05
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Re: Where Spores Are Deposited [Re: Reject]
    #3852207 - 03/01/05 03:19 PM (11 years, 9 months ago)

shakeing the jars is going to pack everything down and you don't want that. you want nice airy substrate.


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OfflineReject
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Re: Where Spores Are Deposited [Re: thenewguy05]
    #3852228 - 03/01/05 03:24 PM (11 years, 9 months ago)

plus pf jars have the dry verm layer at the top as a contam barrier... you don't want to get moisture up in there


--------------------
Any and all references to growing are completely facticious, all pictures shown unless specifically stated are stock photographs found via google image search.


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OfflineCray
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Re: Where Spores Are Deposited [Re: thenewguy05]
    #3852234 - 03/01/05 03:25 PM (11 years, 9 months ago)

how long does it useally take to cool down?


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OfflineCray
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Re: Where Spores Are Deposited [Re: thenewguy05]
    #3852237 - 03/01/05 03:25 PM (11 years, 9 months ago)

how long does it useally take for the jars to cool down? (1/2 pint)

-Cray
*n00b*


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OfflineCiv
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Re: Where Spores Are Deposited [Re: ]
    #3852261 - 03/01/05 03:31 PM (11 years, 9 months ago)

Quote:

I think I'm going to go ahead and shake them to help spread out the spores.




Don't do that - shaking your newly innoc' PF-TEK cakes will mess em up.

Shaking is for grains. Since you can't shake, you use four innoc spots.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


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Anonymous

Re: Where Spores Are Deposited [Re: Civ]
    #3852274 - 03/01/05 03:34 PM (11 years, 9 months ago)

Haha alright..I won't shake them. Thanks everyone.

Cray, I'm pretty sure 24 hours is sufficient time to let jars cool.


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OfflineHambo
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Re: Where Spores Are Deposited [Re: ]
    #3854545 - 03/01/05 11:35 PM (11 years, 9 months ago)

I stick my jars in the freezer for an hour or so, being an impatient twit.


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Invisiblep4kSouL
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Re: Where Spores Are Deposited [Re: Hambo]
    #3854629 - 03/01/05 11:51 PM (11 years, 9 months ago)

i always have problems with injecting my jars with syringes. Something always like gets stuck and then i push down to hard squirting like 2 ccs in one hole ahh!


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OfflineHambo
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Re: Where Spores Are Deposited [Re: p4kSouL]
    #3855434 - 03/02/05 03:15 AM (11 years, 9 months ago)

Thats another good reason I use the method I mentioned earlier :smile:


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