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Offlinekeepsdv8nu
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Registered: 02/28/05
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Psilocybin Poroducer's Guide questions
    #3846228 - 02/28/05 12:53 PM (12 years, 2 months ago)

i've read this doc on numerous sites but certain questions nagged me. i assume that since it lists numerous species of mushrooms that any of these can be substituted for cubensis.
1)If a researcher was to substitute azurescens or cyanescens using this tek, what changes would one have to make?
2)If the researcher was interested in a product being potent and stable, not so much on purity. is the procedure illustrated in the tek decent?
3) Has anyone encountered a better method or made any modifications to this tek?

one, KeepsDv8nU


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Invisiblecricket
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Re: Psilocybin Poroducer's Guide questions [Re: keepsdv8nu]
    #3846249 - 02/28/05 12:59 PM (12 years, 2 months ago)

I did this with Cubes for years. I never tried any others.
Some people here have said that some shrooms are inactive in liquid medium.
Start with Cubes and try some others.


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I tried to leave my signature but it didn't work...
By the way... Does anybody know how to get sharpie markers off of a computer screen?


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Offlinekeepsdv8nu
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Re: Psilocybin Poroducer's Guide questions [Re: cricket]
    #3851188 - 03/01/05 11:18 AM (12 years, 2 months ago)

i read that one can grow strong azurescen mycelia on 1% Malt Agar Extract. the way Stamets broke it down, the mycelia lost potency with a higher concentration of malt extract. this might be a stupid question but what mathematical formula would you use to determine how many grams of malt extract/agar/ml of water to get a 1% malt extrat solution or a 2% malt extract solution for that matter?


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OfflineCryptoman
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Re: Psilocybin Poroducer's Guide questions [Re: keepsdv8nu]
    #3860359 - 03/03/05 12:44 AM (12 years, 2 months ago)

When a percent concentration is given, it usually means weight/volume (w/v). So, 1 gram of malt extract in 100 ml or 10 g in a liter equals 1%. This seems rather low as the Producer's Guide calls for 2%.

On a side issue, has anyone here used a refractometer to monitor carbohydrate consumption in liquid mycelial cultures?


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Offlinedmc_
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Re: Psilocybin Poroducer's Guide questions [Re: Cryptoman]
    #3860415 - 03/03/05 01:00 AM (12 years, 2 months ago)

Impliment different liquid media for your spawn so the culture will recieve different essential nutrients and not become weak.


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Invisiblemjshroomer
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Registered: 07/22/99
Posts: 13,774
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Re: Psilocybin Poroducer's Guide questions [Re: keepsdv8nu]
    #3862948 - 03/03/05 04:37 PM (12 years, 2 months ago)

This guide has numerous errors int he text regarding shroom ID. The author, Gottleib copied his tek from others.

Also Azurescens and cyanescens are cold weather wood chip loving species, they do not do well in dung.

which is for warmer weather species.

mj


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