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OfflineRJLR
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Registered: 02/11/05
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Agar Question
    #3846183 - 02/28/05 12:42 PM (12 years, 2 months ago)

I think my agar is contaminated. There are little yellow brown marks bubbling up on the surface, the mycelium is still growing alright though. In one case I cut some out in the hope that it would not spread, two days later the dish was really quite full of condensation so I've got it upside in the hope it will be alright... Do you think I should chuck the whole lot, and start again... or is it to early to tell? Thanks for any help you can give me...


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OfflineHotnuts
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Re: Agar Question [Re: RJLR]
    #3846429 - 02/28/05 01:54 PM (12 years, 2 months ago)

It sounds like contamination. I know a cool tek if you have a decent amount of healthy mycelical tissue growing. Simply pour another layer of hot agar on top of the growth in your contaminated dish, cover all of the first layer. Sounds crazy doesn't it? But it works great! The layer of hot agar you pour on top of the growth will act like a shield from the contams. The mycelium will crawl through to the top layer leaving the contamination behind. This tek is from Stephen Peele, one of the best! Pour the new layer at 140 degrees. Don't worry about killing the mycelium. It will recover from the heat. Give it a try, it's truly awesome!


Edited by hotnutz (02/28/05 01:55 PM)


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OfflineTantalus
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Re: Agar Question [Re: Hotnuts]
    #3846515 - 02/28/05 02:10 PM (12 years, 2 months ago)

Didn't that incorporate H2O2 in the new agar? Or maybe it's just a different tek.


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"A nation's hope of lasting peace cannot be firmly based upon any race in armaments but rather upon just relations and honest understanding with all other nations...

Every gun that is made, every warship launched, every rocket fired signifies, in the final sense, a theft from those who hunger and are not fed, those who are cold and are not clothed... The cost of one modern heavy bomber is this: a modern brick school in more than 30 cities. We pay for a single fighter with a half million bushels of wheat. We pay for a single destroyer with new homes that could have housed more than 8,000 people..."

President Dwight Eisenhower, 1953


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OfflineHotnuts
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Re: Agar Question [Re: Tantalus]
    #3846910 - 02/28/05 03:30 PM (12 years, 2 months ago)

H202 is only effective with some contams. Trich isn't one of them for example. I always shoot 3 mills of H202 into my agar when i first prepare it and mix it well. That''s all you need. The healthy mycelium will crawl through to the top layer and leave the contaminate behind on the first. Stephen L. Peele's hot agar tek is the sheot. Try it out. You'll love it.


Edited by hotnutz (02/28/05 03:32 PM)


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