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 Workman
1999 Spore War Veteran
 
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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 Isolation of mycelium from dried mushrooms - Fruiting update 
#3762775 - 02/10/05 04:10 PM (19 years, 2 months ago) |
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Too bad there is so much condensation on these plates, but you can still get the idea.
Update 2/18/05
Well, that nice white mycelium isn't mushroom mycelium after all. A quick look under the scope revealed these odd looking structures.
They are the conidia of a species of Pestalotia, a genus of fungi that often causes disease in plants.
Pestalotiopsis link for more information.
It is a bummer and a bit embarrassing but such is the nature of science. I will redo the experiment with agar dosed with benlate fungicide to inhibit sporulating molds and allow the mushroom spores to germinate. Back to the drawing board.
Edited by Workman (05/02/05 03:33 PM)
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Kevin
IES
Registered: 06/03/00
Posts: 676
Loc: Oregon
Last seen: 13 years, 9 months
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3762907 - 02/10/05 04:36 PM (19 years, 2 months ago) |
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Nice man. 
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"Is it a mile walking, or a mile driving?" - dobie
 
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 Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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 Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3763421 - 02/10/05 06:06 PM (19 years, 2 months ago) |
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Since a gill fragment was taken, couldn't this be the germination of spores from a dried specimen versus the isolation of viable mycelium from a dried specimen?
Regardless though, this is good for people to know.
Joshua
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Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Joshua]
#3763907 - 02/10/05 07:23 PM (19 years, 2 months ago) |
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Hmmm, yeah I can see how that is a bit confusing. I guess I should have said, "isolation of viable mycelium from spores attached to the gills of a dried mushroom".
Thanks for pointing that out.
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dmc_
211
Registered: 12/23/03
Posts: 559
Loc: Portland, OR
Last seen: 18 years, 6 months
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3764060 - 02/10/05 07:51 PM (19 years, 2 months ago) |
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Awesome picture workman.
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YidakiMan
Stranger
Registered: 09/28/02
Posts: 2,023
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: dmc_]
#3764370 - 02/10/05 09:56 PM (19 years, 2 months ago) |
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Very cool. I am going to use this to get a culture of V. volvacea.
Did you use any antibiotic or hydrogen peroxide?
Edited by YidakiMan (02/10/05 09:56 PM)
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Fluxburn
.
Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA 
Last seen: 13 years, 4 months
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: YidakiMan]
#3764820 - 02/11/05 12:01 AM (19 years, 2 months ago) |
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wow workman I wish I knew similar to you so I could be your assistant, to bad for me.
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ABSTRACT ART (Mine) http://nathanbelomy.com
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: YidakiMan]
#3766614 - 02/11/05 12:56 PM (19 years, 2 months ago) |
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Oh yes, I used Gentamycin sulfate antibiotic in the agar. Sorry, I should have mentioned that before. I posted that image pretty late in the evening and it seems I left a lot of information out.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3768116 - 02/11/05 06:54 PM (19 years, 2 months ago) |
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I am always glad to see your work!
Joshua
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The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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Re: Isolation of mycelium from dried mushrooms - Contamination update [Re: Workman]
#3806580 - 02/20/05 12:15 PM (19 years, 1 month ago) |
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Oops, edited the top post to reveal a tactical error.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 3 months
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Re: Isolation of mycelium from dried mushrooms - Contamination update [Re: Workman]
#3807219 - 02/20/05 03:21 PM (19 years, 1 month ago) |
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those are funky-looking thingies, workman, if unwanted. the antennae-thingies make them look like they're insects 
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buh
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debianlinux
Myconerd - DBK
Registered: 12/09/02
Posts: 8,334
Loc: Over There
Last seen: 9 months, 17 days
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Joshua]
#3827423 - 02/24/05 12:59 PM (19 years, 1 month ago) |
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Quote:
Joshua said:
I am always glad to see your work!
Joshua
agreed, i always ogle in amazement
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: debianlinux]
#3837982 - 02/26/05 04:40 PM (19 years, 1 month ago) |
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Any updates?
When I first did this, I only used dry tissue from the stipe, to avoid getting spores. I wanted to make sure it was the mycelium recovering, not spores germinating.
Those are some cool looking cells, even if a contaminant. May I ask what type camera you're using?
The antenna looking things are known as cilia. They are used to create movement of the fluids around the cell.
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: RogerRabbit]
#3842777 - 02/27/05 06:04 PM (19 years, 1 month ago) |
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2 days ago I put the gill tissue from several specimens on malt agar with benlate and gentamycin. Nothing to report yet. But I will update this post when something happens.
The camera is an old Sony Mavica FD87.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Mycelium5150
Check theDate!!!!
Registered: 04/05/99
Posts: 541
Last seen: 19 years, 1 month
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3843362 - 02/27/05 07:48 PM (19 years, 1 month ago) |
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you still have that mavica! Isnt that the one that copies pics to floppies
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Mad skills, you know this!!!
I am here to Myth Bust
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Mycelium5150]
#3846830 - 02/28/05 01:12 PM (19 years, 1 month ago) |
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Yeah, the FD stands for Floppy disk. I have a Kodak DX4330 as well but the Kodak is just very handy to knock out web quality pics.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 9 hours, 49 minutes
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3892742 - 03/09/05 12:58 PM (19 years, 1 month ago) |
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The benlate (10mg/liter) gentamycin sulfate (100mg/liter) attempt worked great. No signs of molds or bacteria at all. A vast difference compared to the earlier attempt with only gentamycin.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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YidakiMan
Stranger
Registered: 09/28/02
Posts: 2,023
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3892792 - 03/09/05 01:11 PM (19 years, 1 month ago) |
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Are there any domestic stores that sell benlate? Feed stores?
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Cyber
Ash
Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 11 months, 3 days
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: YidakiMan]
#3892816 - 03/09/05 01:18 PM (19 years, 1 month ago) |
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I looked up benlate and found all kinds of stuff, Pulled from the market in 2001 because of birth defects! Kids born with out eyes, missing limbs, etc. Where did you get it?
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 4 days, 8 hours
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Cyber]
#3892821 - 03/09/05 01:19 PM (19 years, 1 month ago) |
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Before 2001 in the store I guess.
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