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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Pressure Cooker, Wild Bird Seed

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Offlinetrippingchef
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Registered: 01/30/05
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All Contams! What Happened?
    #3837986 - 02/26/05 06:41 PM (11 years, 9 months ago)

I just looked at my jars in the incubator and they have all contamed. I dont know what I did wrong. I used the Pf Tek and the flow hood oven tek and used two layers of tin foil and heated the needle of the syringe. Any ideas?


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OfflineDiMiTriSouljah
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Re: All Contams! What Happened? [Re: trippingchef]
    #3838072 - 02/26/05 07:01 PM (11 years, 9 months ago)

Well, first of all...oven tek sucks. It's known to be reliable for some people, but disastrous for others. I would design a glovebox if I were you...they're dirt-cheap, so there's really no excuse not to make one.

The print/syringe itself could have contained contaminants.


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InvisibleTaskenti
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Re: All Contams! What Happened? [Re: DiMiTriSouljah]
    #3838084 - 02/26/05 07:03 PM (11 years, 9 months ago)

I assume you did sterilize your jars right? How did you sterilize them and for how long?


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Offlinefallingaway24
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Re: All Contams! What Happened? [Re: Taskenti]
    #3838130 - 02/26/05 07:14 PM (11 years, 9 months ago)

bad spores maybe....did u buy them from a shroomery sponsor?


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Offlinetrippingchef
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Re: All Contams! What Happened? [Re: fallingaway24]
    #3838182 - 02/26/05 07:30 PM (11 years, 9 months ago)

I got the spores from Hawks eye. Fortunatly he has a deal that if I mess the first ones up he will give me some more spores. Thank goodness. I heard his spores are good. I presure cooked them for about 25 min. + steam time. Any good links on glove boxes?


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OfflineLethalX5
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Re: All Contams! What Happened? [Re: trippingchef]
    #3838193 - 02/26/05 07:32 PM (11 years, 9 months ago)

Quote:

trippingchef said:
I presure cooked them for about 25 min. + steam time.





There is your prob right there. Cook for atleast 60min at 15PSI man.


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Offlinetrippingchef
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Re: All Contams! What Happened? [Re: LethalX5]
    #3838212 - 02/26/05 07:36 PM (11 years, 9 months ago)

Are you sure? The PF Tek for dummies says for 45. I out a post up before saying that I couldn't presure cooke them for long cause of a one of a kind malfunction with the presure cooker. Does that 60 min inlude the steam time on the way to getting presurised? Or is that 60 min under presure?


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Offlinefallingaway24
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Re: All Contams! What Happened? [Re: LethalX5]
    #3838220 - 02/26/05 07:38 PM (11 years, 9 months ago)

i'm not trying to contradict you lethalx5 but i think alot of beginers think when people tell them to pressure cook them at 15 psi for 60 min, i think they take that as put the jars in there close it up and 60 min. from the time they light the stove. start the 60 min after the pressure reaches 15 psi. i may be wrong but thats the impression i've been picking up lately from some of the new people. but anyways man when you get your new spores just clean out your jars realy good and cook them again.


--------------------
"Most people don't know how they're gonna feel from one moment to the next. But a dope fiend has a pretty good idea. All you gotta do is look at the labels on the bottles." ~drugstore cowboy~


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OfflineDiMiTriSouljah
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Re: All Contams! What Happened? [Re: fallingaway24]
    #3838252 - 02/26/05 07:43 PM (11 years, 9 months ago)

Whenever you read about how long to PC something, you can start timing it as soon as it's completely pressurized (15psi). 45 minutes is long enough for PF cakes. 1 hour is minimum for grain.

The Nook's Glovebox Archive


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OfflineLethalX5
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Re: All Contams! What Happened? [Re: fallingaway24]
    #3838508 - 02/26/05 08:37 PM (11 years, 9 months ago)

Quote:

fallingaway24 said:
i'm not trying to contradict you lethalx5 but i think alot of beginers think when people tell them to pressure cook them at 15 psi for 60 min, i think they take that as put the jars in there close it up and 60 min. from the time they light the stove. start the 60 min after the pressure reaches 15 psi. i may be wrong but thats the impression i've been picking up lately from some of the new people. but anyways man when you get your new spores just clean out your jars realy good and cook them again.




you are right man. i should have made that more clear in my post. Nice follow up! you don't start timing until you reach 15psi(which can take a few min to do)


--------------------
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OfflineIGnosticAbhorI
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Re: All Contams! What Happened? [Re: LethalX5]
    #3838542 - 02/26/05 08:46 PM (11 years, 9 months ago)

I had lots of probs. with wbs, ending up Pc'in for 2hours at 15psi, still got green mold, turns out that the spores were bad :thumbdown: , it really sucked to see so my sub. go to shit.
A foaf pc's for 1h 15m at 15psi, gets great results, like 1-20 i think is about right.
Pf cakes, lol, I just boil them for an hour, works Everytime, unless of course the print is contamed  :rolleyes: :crazy2:


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OfflineCray
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Re: All Contams! What Happened? [Re: IGnosticAbhorI]
    #3838657 - 02/26/05 09:23 PM (11 years, 9 months ago)

How do you tell if the spores are bad?


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InvisibleTaskenti
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Re: All Contams! What Happened? [Re: Cray]
    #3838668 - 02/26/05 09:26 PM (11 years, 9 months ago)

if you only pc'd for 25 minutes it wasn't the spores. Where did the contams show up at first?


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OfflineXTCollection
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Registered: 02/23/05
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Re: All Contams! What Happened? [Re: Taskenti]
    #3838689 - 02/26/05 09:29 PM (11 years, 9 months ago)

Also, a good way to know if the syringe is contamed, or your process.

If the contams are at the point of innoc only, then possibly it is the syring.

If the contams come on immediately, before spore germ, usually it made through PC'ing... Usually takes 1-2 days to show. If the contam shows up after germing, they couldve entered when you were innocing.


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InvisibleHeineken
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Registered: 12/21/04
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Re: All Contams! What Happened? [Re: XTCollection]
    #3838914 - 02/26/05 10:43 PM (11 years, 9 months ago)

no fucking way did they make a book called "pf-tek for dummies" thats so awesome!!


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OfflineFluxburn
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Re: All Contams! What Happened? [Re: Heineken]
    #3839444 - 02/27/05 12:45 AM (11 years, 9 months ago)

wbs I have sterilized for around 45-hour and it works pretty good. I find that the cool down time and pressure release takes at least 20 minutes, so at this time it is still going. So like 50 minutes turning off the heat it still is pcin for a least an hour 10 minutes.


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Offlinescatmanrav
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Re: All Contams! What Happened? [Re: Fluxburn]
    #3839632 - 02/27/05 01:39 AM (11 years, 9 months ago)

PF for simple minds:
http://www.fungifun.org/pf/pf_en.htm

If you want to know if its your jars, next time dont inoculate one. If it contams then its not the syringe or spores.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
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OfflineHippieChick
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Re: All Contams! What Happened? [Re: DiMiTriSouljah]
    #3839995 - 02/27/05 04:00 AM (11 years, 9 months ago)

Check your batch #'s.Ryche had a problem with some of his batch's. Tell them the spores and batch numbers of what you used, if the one's you got were any of the one's that were bad,they will gladly take care of the problem,they did for me. They have been nothing but professional and quick to remedy the situation. I will never buy my spores from anyone but the Hawkseye.They really stand behind their product's.


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Edited by HippieChick (02/27/05 05:14 AM)


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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Pressure Cooker, Wild Bird Seed

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