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OfflineAlegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
Potato Dextrose Agar X Malt Extract Agar
    #3832183 - 02/25/05 08:45 AM (19 years, 1 month ago)

Will PDA work as well as MEA for culture os Psilocibin Mycelium in petri dishes?

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InvisibleDamn_Skippy
Fell off theWagon

Registered: 01/25/05
Posts: 403
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832219 - 02/25/05 08:56 AM (19 years, 1 month ago)

why not?


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~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."

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OfflineAlegalize
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Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Damn_Skippy]
    #3832221 - 02/25/05 08:56 AM (19 years, 1 month ago)

Don?t know, maybe different kinds of nutrients...

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InvisibleDamn_Skippy
Fell off theWagon

Registered: 01/25/05
Posts: 403
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832225 - 02/25/05 08:57 AM (19 years, 1 month ago)

nope... either will work great


--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832282 - 02/25/05 09:21 AM (19 years, 1 month ago)

PDA is just fine for growing mush myc. Some strains however tend to get cottony or very arieal when grown on this media. But once you move them to grain they improove.


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832305 - 02/25/05 09:38 AM (19 years, 1 month ago)

PDA will. Add a 3 grams of light dry malt & it will go better, IMHO.


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OfflineAlegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
Re: Potato Dextrose Agar X Malt Extract Agar [Re: agar]
    #3832355 - 02/25/05 10:01 AM (19 years, 1 month ago)

Well then... Potato is good, but not as good! That?s what I thought...

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OfflineAlegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832369 - 02/25/05 10:08 AM (19 years, 1 month ago)

3 grams dry malt to what quantity of Agar?

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Invisibleagar
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832548 - 02/25/05 11:03 AM (19 years, 1 month ago)

To this

? pint Canning Jars & (Fool Proof) Potato Agar
? Slice 1/8 inch thick or grate 1 potato into 2 cups distilled water.
? Boil for 30 minutes, strain, add distilled water to make 1 Liter.
? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.
? Simmer while stirring slowly for 10 minutes.
? Pour into 1/2 pints (about ? to 5/8 in. deep)
? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).
? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one)
? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.


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