|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Alegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
|
Potato Dextrose Agar X Malt Extract Agar
#3832183 - 02/25/05 08:45 AM (19 years, 1 month ago) |
|
|
Will PDA work as well as MEA for culture os Psilocibin Mycelium in petri dishes?
|
Damn_Skippy
Fell off theWagon
Registered: 01/25/05
Posts: 403
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
#3832219 - 02/25/05 08:56 AM (19 years, 1 month ago) |
|
|
why not?
-------------------- ~Skipp "I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
|
Alegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Damn_Skippy]
#3832221 - 02/25/05 08:56 AM (19 years, 1 month ago) |
|
|
Don?t know, maybe different kinds of nutrients...
|
Damn_Skippy
Fell off theWagon
Registered: 01/25/05
Posts: 403
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
#3832225 - 02/25/05 08:57 AM (19 years, 1 month ago) |
|
|
nope... either will work great
-------------------- ~Skipp "I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
#3832282 - 02/25/05 09:21 AM (19 years, 1 month ago) |
|
|
PDA is just fine for growing mush myc. Some strains however tend to get cottony or very arieal when grown on this media. But once you move them to grain they improove.
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
#3832305 - 02/25/05 09:38 AM (19 years, 1 month ago) |
|
|
PDA will. Add a 3 grams of light dry malt & it will go better, IMHO.
--------------------
|
Alegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: agar]
#3832355 - 02/25/05 10:01 AM (19 years, 1 month ago) |
|
|
Well then... Potato is good, but not as good! That?s what I thought...
|
Alegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 18 years, 11 months
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
#3832369 - 02/25/05 10:08 AM (19 years, 1 month ago) |
|
|
3 grams dry malt to what quantity of Agar?
|
agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
#3832548 - 02/25/05 11:03 AM (19 years, 1 month ago) |
|
|
To this
? pint Canning Jars & (Fool Proof) Potato Agar ? Slice 1/8 inch thick or grate 1 potato into 2 cups distilled water. ? Boil for 30 minutes, strain, add distilled water to make 1 Liter. ? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast. ? Simmer while stirring slowly for 10 minutes. ? Pour into 1/2 pints (about ? to 5/8 in. deep) ? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars). ? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one) ? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.
--------------------
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 6,238 topic views. 11 members, 68 guests and 127 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|