Home | Community | Message Board


RVF Garden Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar

Jump to first unread post. Pages: 1
OfflineAlegalize
Stranger
Registered: 02/23/05
Posts: 16
Last seen: 11 years, 11 months
Gentamicin use in Agar.
    #3831106 - 02/25/05 01:48 AM (12 years, 1 month ago)

I?d like to know what kind of Gentamicin should I use mixede with agar to prevent bacterial contamination, and also if there?s any con using it. I guess the one to use is technically called Gentamicin Sulfate is this right? And it seems I can only get it dissolved in liquid for injection in humans. Like 40mg in 1ml. Thanks in advance!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePsilygirl
cyan goddess
 User Gallery

Registered: 08/28/03
Posts: 4,418
Loc: PNW
Last seen: 4 months, 2 days
Re: Gentamicin use in Agar. [Re: Alegalize]
    #3831446 - 02/25/05 03:33 AM (12 years, 1 month ago)



--------------------
"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."


Puget Sound Mycological Society


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
 User Gallery

Registered: 03/26/03
Posts: 41,989
Loc: USA Mountain Northwest
Last seen: 4 days, 2 hours
Trusted Cultivator
Re: Gentamicin use in Agar. [Re: Psilygirl]
    #3838022 - 02/26/05 06:48 PM (12 years, 30 days ago)

Another trick to use that helps even without antibiotic agar is to simply pour hot agar right on top of a contaminated plate. The heat seems to slow down the molds long enough for the mushroom mycelium to recover and climb up through the layer to break out on top. This usually only takes a day or two. As soon as the myc starts to appear on the top layer, 'shave' a small amount off and transfer to a new dish. Don't take any of the agar, but only scrape a bit of the myc off the top. This seems to leave the contamination behind 90+ percent of the time. Repeat the process if necessary. I've cleaned up lots of edible cultures from the wild this way.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleastrozombi3
atm
 User Gallery

Registered: 11/24/04
Posts: 114
Re: Gentamicin use in Agar. [Re: RogerRabbit]
    #3840052 - 02/27/05 04:22 AM (12 years, 30 days ago)

only sterility prevent bacterial contamination. I don?t recommend antibiotics use. If you want can add 10 mg in your agar recipe before sterilization.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFluxburn
.
 User Gallery

Registered: 10/23/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 6 years, 4 months
Re: Gentamicin use in Agar. [Re: astrozombi3]
    #3874515 - 03/05/05 09:55 PM (12 years, 23 days ago)

Good knowledge... someone kill the green mold arg I hate that shit.


--------------------
ABSTRACT ART (Mine) http://nathanbelomy.com


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
 User Gallery

Registered: 03/26/03
Posts: 41,989
Loc: USA Mountain Northwest
Last seen: 4 days, 2 hours
Trusted Cultivator
Re: Gentamicin use in Agar. [Re: Fluxburn]
    #3875810 - 03/06/05 04:03 AM (12 years, 23 days ago)

Antibiotics aren't for green mold which is an imperfect fungi, they're anti-bacterial. True, sterile technique is foremost, but some of us are using prints that were left under the cap too long and have bacterial rods all over them. When the mushies germinate, so does the bacteria. Antibiotic agar is a godsend, imo.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Does Isolation On Gentamicin Agar Injure Isolates? AdoreChampignons 2,255 7 08/17/08 12:13 PM
by x7x_x7x
* Best Antibiotic for Spore Syringes?
( 1 2 all )
Joshua 2,904 23 06/18/03 04:25 AM
by Joshua
* Plug use and various antibiotics? ucsdmolpath 735 3 01/12/04 06:03 AM
by ragadinks
* Gentamicin in spore syringes? *DELETED* OOKLA 1,505 14 06/21/03 11:51 PM
by RogerRabbit
* Antibiotic concentrations in Agar cheesenoonions 2,088 9 12/30/02 01:40 AM
by Zen Peddler
* Gentamicin Dem_Bones 1,819 13 09/12/07 06:32 PM
by fastfred
* antibiotic agar
( 1 2 all )
THEDANGLER 4,217 28 12/25/08 12:20 AM
by shroomophile
* Using ampicillin instead of gentamicin sulfate LanaM 2,421 12 01/21/04 04:28 PM
by Basidiocarp

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,848 topic views. 0 members, 1 guests and 4 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Avalon Magic Plants
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.036 seconds spending 0.004 seconds on 14 queries.