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OfflineAlegalize
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Registered: 02/23/05
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Gentamicin use in Agar.
    #3831106 - 02/25/05 01:48 AM (12 years, 5 months ago)

I?d like to know what kind of Gentamicin should I use mixede with agar to prevent bacterial contamination, and also if there?s any con using it. I guess the one to use is technically called Gentamicin Sulfate is this right? And it seems I can only get it dissolved in liquid for injection in humans. Like 40mg in 1ml. Thanks in advance!


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OfflinePsilygirl
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Re: Gentamicin use in Agar. [Re: Alegalize]
    #3831446 - 02/25/05 03:33 AM (12 years, 5 months ago)



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OfflineRogerRabbitM
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Re: Gentamicin use in Agar. [Re: Psilygirl]
    #3838022 - 02/26/05 06:48 PM (12 years, 5 months ago)

Another trick to use that helps even without antibiotic agar is to simply pour hot agar right on top of a contaminated plate. The heat seems to slow down the molds long enough for the mushroom mycelium to recover and climb up through the layer to break out on top. This usually only takes a day or two. As soon as the myc starts to appear on the top layer, 'shave' a small amount off and transfer to a new dish. Don't take any of the agar, but only scrape a bit of the myc off the top. This seems to leave the contamination behind 90+ percent of the time. Repeat the process if necessary. I've cleaned up lots of edible cultures from the wild this way.


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Invisibleastrozombi3
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Re: Gentamicin use in Agar. [Re: RogerRabbit]
    #3840052 - 02/27/05 04:22 AM (12 years, 5 months ago)

only sterility prevent bacterial contamination. I don?t recommend antibiotics use. If you want can add 10 mg in your agar recipe before sterilization.


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OfflineFluxburn
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Re: Gentamicin use in Agar. [Re: astrozombi3]
    #3874515 - 03/05/05 09:55 PM (12 years, 5 months ago)

Good knowledge... someone kill the green mold arg I hate that shit.


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OfflineRogerRabbitM
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Re: Gentamicin use in Agar. [Re: Fluxburn]
    #3875810 - 03/06/05 04:03 AM (12 years, 5 months ago)

Antibiotics aren't for green mold which is an imperfect fungi, they're anti-bacterial. True, sterile technique is foremost, but some of us are using prints that were left under the cap too long and have bacterial rods all over them. When the mushies germinate, so does the bacteria. Antibiotic agar is a godsend, imo.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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