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OfflineMuddyMinds
newbie

Registered: 07/22/01
Posts: 39
Last seen: 21 years, 8 months
Innoculation question
    #382645 - 08/31/01 08:51 AM (23 years, 1 month ago)

Using a flow hood...and jars that have 1/2" vermucilite at the top...can one innoculate the jars by opening the top and just squirting the fluid in.

Or using the same setup, can one poke a sterilized item through the top for innoculation, instead of opening the lid.

I sterilized some jars of rye berries and didn't pre poke the innoculation holes...so I'm wondering the best way to avoid contamination. Thanks.



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when the light turns green, you go. when the light turns red, you stop. but what do you do, when the light turns blue with orange and lavander spots?-Shell Silverstein


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*************************************************************
when the light turns green, you go. when the light turns red, you stop. but what do you do, when the light turns blue with orange and lavander spots?-Shell Silverstein

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Anonymous

Re: Innoculation question [Re: MuddyMinds]
    #382654 - 08/31/01 09:08 AM (23 years, 1 month ago)

Unless you are living in some filthy pig sty, you should be able to jsut take the lid off, quickly innoculate, then put lid back on immediately.

I've done it this way many times with no problems. No holes, no tape, no flame sterilization....no flow hood! And not a problem.

Take a trip to the Spore Lab @:
http://www.SporeLab.com
email: getspores@sporelab.com

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OfflineTheReverend
journeyman
Registered: 08/17/01
Posts: 69
Last seen: 22 years, 10 months
Re: Innoculation question [Re: MuddyMinds]
    #382660 - 08/31/01 09:20 AM (23 years, 1 month ago)

The "oven tek" works well. Just heat your oven to about 150?, open the door and use it as your work area, then you can do the transfers w/out too much fear of contams.

.
.
Fuck art, let's kill!

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Offlinekingkc
enthusiast
 User Gallery
Registered: 04/24/00
Posts: 1,064
Last seen: 6 years, 5 months
Re: Innoculation question [Re: TheReverend]
    #382746 - 08/31/01 12:38 PM (23 years, 1 month ago)

Captain is right . Just be cautious and careful and you should be ok. Pay attention to detail. Good luck. If you've got a hood then you're not going many probs. Peace.


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