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Invisiblep4kSouL
Animals Are Cool
Registered: 01/13/05
Posts: 3,666
sterilizing my syringe
    #3819435 - 02/22/05 11:22 PM (12 years, 6 days ago)

Ok so I am trying to sterilize my used plastic syringe-

I boiled some water and sucked it in and squirted it into the sync 3 times.

Then I sucked in some rubbing alcohol and shook it and squirted that shit out.

Then I sucked in some more of the boiling water 3 more times and squirted it out.

Think its sterile? Also I have a question about how good rubbing alcohol can kill contams. Does it immediately kill contams on contact? After I sucked in the spore fluid I soaked the syringe needle with alcohol then put the alcohol soaked lid on the needle. I hope its sterile.


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Invisibledobinky
infoseeker
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Registered: 01/30/03
Posts: 2,436
Loc: Wiki, Kentucky
Re: sterilizing my syringe [Re: p4kSouL]
    #3819462 - 02/22/05 11:25 PM (12 years, 6 days ago)

you could skip the alky step as the boiling water will kill everything.


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InvisibleHolydiver
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Registered: 03/20/01
Posts: 5,156
Loc: The midnight sea
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Re: sterilizing my syringe [Re: p4kSouL]
    #3819477 - 02/22/05 11:27 PM (12 years, 6 days ago)

Your process will work, but you didn't need that middle extra alcohol step. 1-3 times sucking in boiling water and let sit for a few is all you need.

There is nothing to it, you got it down. Most people way overdo it with a million steps, and finishing it off with an alcohol flame. Want to know something? I never use flame sterilization--alcohol is all you need. As far as I know, yes, it kills on contact.


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To find a place to live between the negatives and positives.


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OfflineKada
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Registered: 02/15/05
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Re: sterilizing my syringe [Re: p4kSouL]
    #3819510 - 02/22/05 11:30 PM (12 years, 6 days ago)

Well, i would say thats over board, i personnaly never use alcohol. I rinse off anything on it, suck up some water, wrap the sucker in alluminum foil and boil it for 20 min, take it out let it cool. BAM* contaim free water for my spores and a steril needle and i store. When using, heat needle with my burner and push some water thu it. then push the rest into a sterilized shot glass with me spores in it, suck up and burn my needle again before i inject cakes. just my way, not only way.


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~The Cultivators Motherload~

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them.
I am free because I know that I alone am morally responsible for everything I do." -Robert A. Heinlein

"There is no need for temples, no need for complicated philosophies.
My brain and my heart are my temples; my philosophy is kindness."-Dalai Lama

Live long and prosper.



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Offlinepsilocyben
bad guy

Registered: 12/03/04
Posts: 1,026
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x [Re: Kada]
    #3819767 - 02/23/05 12:04 AM (12 years, 6 days ago)

me=advocate of alcohol sterilization

but...
someone made a good point one time that you should initially flame sterilize the needle before beginning for the sake of anything inside the needle.
i simply put the cap on with h202 in it, so i shouldn't need it.
but of course do it as a precautionary method anyway.
there is a thin line between caution and paranoia- it doesn't hurt to cross that line every once in a while.


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
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Re: x [Re: psilocyben]
    #3819849 - 02/23/05 12:17 AM (12 years, 6 days ago)

After you sterilize, should you draw in some superheated air or something? it just seems like after you squirt out the last of the liquid u use to sterilize, this would leave room for air contamination. If the syringe is in its fully depressed state, then air can get to the inner walls through the small gap between the plunger and the shell of the syringe. Does this matter since it seals when you draw up liquid?


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: x [Re: LengthyFellow]
    #3834967 - 02/25/05 11:41 PM (12 years, 3 days ago)

bump.


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Re: x [Re: LengthyFellow]
    #3835137 - 02/26/05 12:30 AM (12 years, 3 days ago)

if you're reusing the suringe, sterilize it with boiling water, suck and squirt water thats been boiling for a few minutes about 3-4 times


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OfflineKaptKid
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Re: x [Re: Prisoner#1]
    #3835261 - 02/26/05 12:58 AM (12 years, 3 days ago)



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Offlinerocknliam
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Registered: 12/12/03
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Re: x [Re: KaptKid]
    #3835514 - 02/26/05 02:31 AM (12 years, 2 days ago)

I sucked boiled water in and out a few times on my second grow, and got one jar with a tiny contam. The first time i pulled in about 1-2cc of water and nuked it for 5 mins, and got zero contams. they are just as good as each other, but the boiling water was easier.


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OfflineSilverr
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Registered: 06/10/05
Posts: 95
Last seen: 7 years, 6 months
Re: x [Re: rocknliam]
    #4455197 - 07/26/05 01:20 PM (11 years, 7 months ago)

Quote:

rocknliam said:
I sucked boiled water in and out a few times on my second grow, and got one jar with a tiny contam. The first time i pulled in about 1-2cc of water and nuked it for 5 mins, and got zero contams. they are just as good as each other, but the boiling water was easier.




If anyone ever reads this post again please note that it has been tested that any H2O heated 3min+ (beyond boiling point [depending on your microwave]) will EXPLODE on contact with any metal substance such as silverware (fork, spoon, etc.), so be careful when nuking.

PEACE, TC :grin:


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"The only easy day was yesterday"


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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