Temperature drop relates to moisture content and relative humidity and this is the coincidence. Morels are cultivated on the concept I am arguing. Mycelium is completley deprived of nutrient base, to form sclerotia, in the abscense of nutrient, based on the nutrient they have already consumed. These sclerotia are fruited.
Cubensis is subtropical, and my arguments where based on
WARM WEATHER MUSHROOMS. Stametes as you know stated also that warm weather species do not conform to
Temperature induction. I stated that temperature plays an increased role in cold weather species. They will not fruit above or below a certain temperature range.
Cubensis on the other hand will fruit in a high CO2 environment, at hot temperatures. The only variable that can be left is NUTRIENT DEPRIVATION. Now strains within the species have higher and lower sensitivities to CO2 and Temperature, but as a whole, they will initiate pins invitro regardless of a temperature drop or high CO2, but only after they have used up the available nutrients, and have completed their colonization of the substrate. This my friends can only be linked to NUTIENT DEPRIVATION, because all other variables have remained constant.
You can call it coincidence, but it is reproducible, and would hold up in any Scientific Journal.
All of your quotes from stametes are related to COLD WEATHER SPECIES. And each and every quote relates Temperature in combination with OTHER signals. Not on it's own. My statement has isolated all triggers, the only thing that has changed is Nutrient Availability. My scientific study is sound and reproducible, it is not based on combinations of temperature and .......... Only on the lack of nutrient availability without other stimuli. I am not disagreeing with stametes, re read his books.
" Strains isolated from subtropical or tropical climates generally do not require a cold shock." Paul Stametes, GGMM.
If you want a concentration, well I am gonna need a Lab with some equipment.
Temperature is related to other environmental factors. When temperatures fall, it usually rained, and is followed by drier air, hence the evaporation rate is increased. All of these factors are crucial for maturation, but not neccesary for initiation in CUBENSIS.
Once the colony has been established, you can remove all nutrients, and the shrooms are still gonna grow up, they are not consuming nutrients, they are absorbing water. They are not like plants, and the mycelium is the roots. Once a substantial mass has been achieved, the size of the mushrooms is in relation to that quantity of mycelial mass. Because they are not growing more mycelium, the mushrooms are formed from the mycelium already present. The mycelium is woven by the hyphal knot into a pin, this pin swells with water. Water in the wild is associated with a decrease in temperature, and the following decrease in relative humidity. These factors mature the pins. If you are a mycelium growing in colder climates, you would want to fruit at colder temperatures, so that your mycelial mass could grow as large as possible before fruiting. The more mycelial mass the more fruits.
Subtropical shrooms have shorter life spans, they grow quickly, need very little if any rest, and they fruit when moisture is available, followed by a lower relative humidity. Even the latter is probably very insignificant, the relative humidity in the swelling mushroom is probably close to a 100 percent, so anything less in the air, will cause a gradient sufficient to expand the mushrooms. Cubensis is not Azurescens. There life cycle looks the same in a diagramn, but it is very different.
Pins form invitro on Nutrient agar after a sufficient amount of time has passed, to obtain enough mass, and to consume the majority of available nutrients. Nutrients are inside the agar, but the mycelium is not, it gets harder and harder to obtain the remaining nutrients, hence the deprivation, and subsequent pin set, regardless of lowered temperatures or CO2.
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