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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Hydrapod test in progress [Re: Jammer]
    #389325 -

First of all, no one here is suggesting that the thing does not work, LET"S GET THAT CLEAR!!!
We are discussing various aspects of the grow cycle and how that relates to the POD. I have never said it does not work, nor am I suggesting that. A user of the pod is having trouble getting mature shrooms, and MCMANS post clearly explained many reasons why it might not be working. We are certainly not doubting MCMAN.
My personal interest had very little to do with why they are not maturing, that is obvious!!! EVAPORATION RATE!! Which temperature, humidity, and air exchange all play roles in. My concern or more of an interest was in how the mycelium does not grow into the geo-lite, and I am really interested in how it does not when Extra nutrients are added into the reservoir. MCMAN said he had tried that before, and the mycelium did not grow into the geo-lite. I was curious what the invisible barrier was, O2, the geo-lite itself, ect... but the only answer I have gotten was temperature. This answer does not explain it for me!!!! Because mycelium will continue to grow at very low temperatures, and very high temperatures. This is completley seperate from the temperature requirements needed to maintain the pod itself!!! The pod is an enclosed system and needs to be used at the recommended parameters, and if I were anyone using it, I would certainly follow the directions!!!!
TEMPERATURES at 68 F outside the system, are not sufficient to halt the mycelium from growing. Reduced nutrients, and exposure to high levels of Oxygen, will on the other hand form a sufficient barrier to continual mycelial growth. I guess that is the thing I was really wondering. I am absolutely positive Temperature is not the reason for the mycelium dying back in the straw cake observed in MCMANS post. What is the barrier? To wet, to much O2, the geo-lite, these are realistic possibilities, not Temperature.


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    #389396 -


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Re: Hydrapod test in progress [Re: McMan]
    #389455 -

Sorry guys. Im just another hippie that has surfed other type related sites. I too think that my last responce on this thread was directed to another user too... It's easy to get confused with so many handles. Anyway, if anyone cares, Im a regular "joe" that works a white collor 9-5 and spends most of my spare time playing music and surfing sites.
I had the misfortune in the past of beliveing that perscription pain killers were a good buzz... So after nearly killing myself with the pills, nearly 10 years ago, I feel more comfortable around other fellow "heads" and 'shroomers. I hope thats cool....

Later
Jammer



--------------------
>>Jammer>>

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Re: Hydrapod test in progress [Re: Jammer]
    #389457 -

I just wanted to point out that if any of my recent responces dont seem to quite fit into the thread flow that it's because I seem to have misread the threads.... man we have a lot of users on this site! I mean it's like... I GET SO CONFUSED SOMETIMES!! ... heheh - so please disregaurd if any of my recent replies dont seem to apply. Thanks.

Jammer



--------------------
>>Jammer>>

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Re: Hydrapod test in progress [Re: Jammer]
    #389804 -

WHoa jammer,,, your like,,,, ummm baked.

D D Island

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Deleted [Re: ]
    #389845 -

Content Removed.

Edited by Jared (04/01/04 01:11 AM)

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Post deleted by users_request [Re: Jared]
    #389849 -


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Re: Hydrapod test in progress [Re: Workman]
    #389867 -

Day 11: After reducing the flood drain rate and lowering humidity a pin shows up on the Ecuador cake. The picture detail is poor but it is there, on the pure white cake in front, that small dot near the top edge, yeah you see it, that one.

I don't require or want a refund. The pod is well made and appears to be theoretically sound in design. I am sure I just need to work out the adjustments for my home environment.



The Spore Works
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My Instagram
Reinvesting Sales Towards Basic Research and Species Identification :amanitajar:

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Re: Hydrapod test in progress *DELETED* [Re: Workman]
    #389872 -

Post deleted by jheck

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Post deleted by users_request [Re: jheck]
    #389902 -


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Re: Hydrapod test in progress [Re: McMan]
    #389935 -

MCMAN
I germinate some cubensis spores on a plate. Transfer a couple of times, wind up with a pure strain. I forget about a couple of them in a box. The temperature throuout the process never goes up or down, but remains at a constant 85 F. Well some time goes by and I remember the plates. I remove them from the box, about 25 days have passed, and the mycelium has started to fill in all the available space between the now drasticaly depleted agar media, and the lid of the sealed petri. The funny thing is there are pins all over the place.
Everything has remained constant, the high humidity, high CO2 levels, and the temperature has remained the same. Why do I have pins? What has changed?
Answer: the nutritional content of the media. The mycelium has begun to completely deplete the petri dish of food. Only alternative, Reproduce!!!!
If I leave a spawn jar at 85 degrees for five days and lower the temperature to 75 or 70, it will take longer to colonize, but does it stop growing and automatically fruit. Maybe if I remove it from the jar, lay it out in a tray, therefore reducing CO2, and maintain adequate moisture. But invitro without the other environmental factors to help initiate.
So yes I guess we disagree. I think Temperature plays a role in the rate of growth, and can have stimulatory effects on fruiting, but it is not the primary factor in initiating pins of at least P. cubensis. I am sure many other species would also have this ability to pin at constant temperatures above any "shock" temperature, invitro, under high CO2 and humidity.
NOW mushroom developement is an entirely different story. That's where the evaporation rate becomes so important. And that is certainly tied in with Gas exchange, and moisture, far more importantly than nutrition, which was the basis for are last discussion. Mushrooms being a product of expansion not growth, relying most importantly on the availability of moisture, not nutrients.
Peace Teonan


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    #389954 -


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Re: Hydrapod test in progress [Re: McMan]
    #390367 -

No, I do not believe that cubensis will fruit invitro by lowering temperatures only. If CO2, is high, and there are available nutrients, i.e the jar is not completely colonized, they will not fruit. Temperature is a stimulus in fruiting, not a dependent factor, with cubensis. Temperature regulates the growth RATE not life cycle. So I think we still disagree. I will agree that mycelium will grow quickest at 86F for cubensis, but it will continue to grow vegetatively at 70 F if the CO2 remains high, and there is still food to eat. This is what I am trying to clearify. Temperature relates primarily to growth rate, because if all other variables remain constant, except temperature, fruiting will not automatically start. But if the same experiment is done, but all variables remain constant, and only nutrient deprivation occurs, they will automaticaly fruit. This leads me to believe that with cubensis, Temperature by itself is only a growth rate Factor. Where as nutrient deprivation is a determining factor in Pin initiation.
When you lower the temperatures you are not neccessarily telling the colony it is time to fruit, you are slowing it's metabolism. Hence the advantage with overlay. But you are not stopping Veg. growth, only slowing it. When temperatures are high, the Growth rate of the mycelium speeds up, but you are not signaling it to stop fruiting. It will continue to fruit, if nutrients are depleted. You will however force the mycelium back into Veg. growth if more nutrients are added, and Co2 levels rise again. All in disregard to the temperature. It will happen slower or faster in relation to temperature. Again we are discussing Cubensis.
Now I do believe Temperature has a strong effect on the quality of fruits, but it is not a determining factor of Pin initiation. The experiment suggested in the last post, Invitro at a constant temperature, proves that. Don't look at it like it was a fruit or die situation, look at it for what it represents. A controlled experiment, tying in GAS EXCHANGE,TEMPERATURE, MOISTURE CONTENT, HUMIDITY, AND NUTRIENTS; and the effect these things have on the initiation of Pins in Cubensis. The only factor present was the depletion of nutrients. Temperature was basicaly irrelevant, High Co2 levels and humidity also irrelevant. Only Nutrient deprivation and/or depletion played a role. Turn the experiment inside out, you will find similar results. Put a pure strain on a petri and lower temperatures but keep everything else constant. The mycelium will slow down it's veg growth, nutrients will still be available, other factors are constant, but no Pins. The mycelium continues to grow slowly across the plate, until it's nutrients are depleted, then it fruits.
Peace Teonan


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Re: Hydrapod test in progress [Re: ]
    #390380 -

Now cold weatherspecies who knows? I think if it was studied it would be determined that temperature has an indirect stimulus factor here also. Possibly just relating to a slow down in the metabolism of the mycelium to the point of nutrient unavailability? Alot of studies need to be done? And as I said before Research is limited into edible mushrooms, certainly at the University level. As you stated Privately there is research being done, but I think at the private level it is cost ineffective to study such things. They want to know how much Yield can be Had in the least amount of time from the least amount of space!!!!!! Correct? They are not researching for the sake of research!!!
Temperature definetly plays a larger role in the Life Cycle of cold weather species, but I do believe it is indirect, like the above situation.
Peace Teonan


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Re: Hydrapod test in progress [Re: ]
    #390437 -

no offence, but i never saw a mushroom grown on the mcman hydo pod yet



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Re: Hydrapod test in progress [Re: humplok9]
    #390470 -

There is a picture of one in this thread!!!!!! And if I were MCMAN I wouldn't post any more either. That was the point of giving one a way for testing. So someone could post results. He is now a vendor, and it is not in his best interest to post pictures of illegal activity with a product he is selling being used!!!! Pay attention to what is being said in the exchange between us, we are not arguing the functionality of his POD. I'm certain it works!!!!! Why wouldn't it?
OOPS pictures are in the BETA TESTER THREAD at the forest floor.

Edited by Teonan on 09/10/01 06:27 PM.


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    #390540 -


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Re: Hydrapod test in progress [Re: McMan]
    #390711 -

No problem, MCMAN, I too enjoy these conversations.
Yes Temperature controls growth rate . Yes Mycelium grows FASTEST at HIGHER TEMPERATURES, but so will shrooms.
The trigger from Veg. to Fruit is primarily one of Nutrient Depletion and /or Deprivation of substrate.
Optimum Pin set will be determined by making available fresh Air, proper moisture content, and lowered temperatures ( which will occur inside the substrate itself upon depletion of nutrients) at the right timing, which will be determined by the depletion of nutrients from the substrate. If you can time all this right, you get optimum pin set.
Lowering temps on it's own without proper air exchange will not triggerPins.

P.S. those P. ricans will still veg at low temps. It is just very aggressive. I still think the no pinning was more related to Air exchange than temperature. But if you have to lower the temperature to get the proper exchange, then DO IT!!!! I never fruit cubies below 75 degrees. But I live in The DEEP SOUTH, sunny old MIAMI. It would cost a small fortune to run the AC that much!!!
Peace Teonan



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Deleted [Re: McMan]
    #390726 -

Content Removed.

Edited by Jared (04/01/04 01:11 AM)

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    #390860 -


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