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 Damn_Skippy
Fell off theWagon
Registered: 01/25/05
Posts: 403
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 G2G question 
#3810990 - 02/21/05 02:53 PM (13 years, 2 months ago) |
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Hypothetical scenario...
Let's say I have 4 rye jars. 2 Lipa Yai and 2 Hawaiian. After 2 and half weeks I have one Lipa Yai and one hawaiian jar 1/3rd of the way colonized and moving fast. The uncolonized jars have been noc'd but no growth. I'm guessing conditions aren't favorable for colonization in the uncolonized jars. However, if the 2 colonizing jars colonize completely before the other jars have started to colonize, is it possible to take some of the Myc from these Rye jars that have colonized and do a G2G into the uncolonized jar even though conditions might not be favorable for growth in those particular jars? has anyone tried it? will it give it the kick start it needs?
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~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
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 brewwhaha1
Stranger thanmost
Registered: 02/13/05
Posts: 253
Last seen: 12 years, 4 months
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give it a try does the grain from both jars (growing not growing) appear to be the same moisture content?
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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yes it's possible but you may want to try with new jars, those unfavorable conditions could be indicative of a bacterial contam or something similar.
you'll also need a glove box or a flow hood
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DiMiTriSouljah
No left turn unstoned
Registered: 07/02/03
Posts: 1,120
Loc: ked in a Skin Pinata
Last seen: 3 years, 4 months
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I wouldn't risk the contamination rate of opening partially colonized rye jars. Unless you're pretty confident that you can pull it off, it isn't worth contaminating 4 jars instead of losing 2. But, hypothetically, yes, it's definitely possible.
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In the end, my friend, we will all be together again.
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Damn_Skippy
Fell off theWagon
Registered: 01/25/05
Posts: 403
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Hypothetically experimenting...
the uncolonized jars are slightly drier, but probably would be injected with sterile water upon G2G. They have all been injected with sterile water once already
--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
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Damn_Skippy
Fell off theWagon
Registered: 01/25/05
Posts: 403
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Quote:
Prisoner#1 said:
yes it's possible but you may want to try with new jars, those unfavorable conditions could be indicative of a bacterial contam or something similar.
you'll also need a glove box or a flow hood
Never had a contam, but I am not denying that these jars don't have one now. It is possible. I have neither a glovebox nor flow hood. So I guess I will be casing with one jar
Thanks Priz
--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
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brewwhaha1
Stranger thanmost
Registered: 02/13/05
Posts: 253
Last seen: 12 years, 4 months
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why does everyone seem so concerned about contamination???? I realize its important to be clean. The only time I've had problems with contamination is when i wasn't PCing long enough (only 45 min @15psi) stupid me it resulted in 100% contamination for 2 batches. I've doubled the time and now no problems at all.
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Damn_Skippy
Fell off theWagon
Registered: 01/25/05
Posts: 403
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Quote:
DiMiTriSouljah said:
I wouldn't risk the contamination rate of opening partially colonized rye jars. Unless you're pretty confident that you can pull it off, it isn't worth contaminating 4 jars instead of losing 2. But, hypothetically, yes, it's definitely possible.
my contam rate is 0, so far. cross my fingers. But you are right. A bird in the hand is worth 2 in the bush
--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
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DiMiTriSouljah
No left turn unstoned
Registered: 07/02/03
Posts: 1,120
Loc: ked in a Skin Pinata
Last seen: 3 years, 4 months
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Because some people live in dirty alleys.
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In the end, my friend, we will all be together again.
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