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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
casing the Panaeolus substrate (pics)
    #380832 - 08/28/01 05:55 PM (15 years, 9 months ago)

After 5 days of incubation the substrate was fully colonised and ready to be cased:

4 bags of substrate:



Close up:



A plastic bin is cleaned with alcohol. (note the holes plugged with polyfill)



The bags are cut open with scissors:



The substrate is put in the bin:





This is levelled:



And cased with strerilised casing soil:





The bin is covered with polyethylene wrap:



Side view:



The bins are now in a warm place for the casing layer to colonise.





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Offlinedioze1
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Re: casing the Panaeolus substrate (pics) [Re: Una]
    #380946 - 08/28/01 08:04 PM (15 years, 9 months ago)

Una,
I love your posts!! I wish that all posts here could be as rich in good, well illustrated info.
My hats off to you and your efforts.
Dioze1

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Invisibleslither
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Registered: 02/03/01
Posts: 365
Re: casing the Panaeolus substrate (pics) [Re: dioze1]
    #381027 - 08/28/01 10:02 PM (15 years, 9 months ago)

Fantastic, wow that's quick colonization, what was your spawn rate per bag, and did you shake the bag any during colonization or just leave it alone?



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: casing the Panaeolus substrate (pics) [Re: slither]
    #381237 - 08/29/01 04:25 AM (15 years, 9 months ago)

Bags were spawned with 300 ml of colonised rye grain. They were shaken every day, because the substrate and the spawn don't mix very easily (grain kernels fall through the substrate and collect at the bottom of the bag). This species really is amazingly fast!

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OfflineCACA
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Re: casing the Panaeolus substrate (pics) [Re: Una]
    #381241 - 08/29/01 04:30 AM (15 years, 9 months ago)

Good work. Might be because I am a nub to shrooms in general, but i am impressed. Nice post work, too.

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OfflineMAIA
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Re: casing the Panaeolus substrate (pics) [Re: Una]
    #381472 - 08/29/01 03:36 PM (15 years, 9 months ago)

300 ml each bag or 300 ml to all bags ?


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: casing the Panaeolus substrate (pics) [Re: MAIA]
    #381510 - 08/29/01 04:46 PM (15 years, 9 months ago)

300 ml to each of the bags

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Offlineuniboner
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Re: casing the Panaeolus substrate (pics) [Re: MAIA]
    #381511 - 08/29/01 04:46 PM (15 years, 9 months ago)

how big are those air holes, and would they provide sufficient air for no fanning to be required? Would the same airhole setup work for cubensis?

Thanks and peace,
uniboner

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OfflineMAIA
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Re: casing the Panaeolus substrate (pics) [Re: uniboner]
    #381549 - 08/29/01 05:56 PM (15 years, 9 months ago)

Why it shouldn't work with P.Cubensis? They also need air exchange.


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Offlineuniboner
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Re: casing the Panaeolus substrate (pics) [Re: MAIA]
    #381557 - 08/29/01 06:04 PM (15 years, 9 months ago)

I was thinking since the pans generally have less surface area, that they may produce less CO2 and therefore require less air exchage.

I was simply wondering if those holes would provide sufficient air exchange for cubes as they seem to have more surface area and may produce more CO2.
But that was just a guess, and that's why I asked.

peace,
-uniboner

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Offlineuniboner
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Re: casing the Panaeolus substrate (pics) [Re: uniboner]
    #382131 - 08/30/01 03:18 PM (15 years, 9 months ago)

Well, I cased another batch last night and I made similar holes and filled with polyfill as pictured above.

I guess Ill see if there's enough air exchange from the holes.
Anyone had any experience with this in the past/present?
please let me know if I should still be fanning and such.

peace,
-uniboner

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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: casing the Panaeolus substrate (pics) [Re: uniboner]
    #382840 - 08/31/01 06:01 PM (15 years, 9 months ago)

One of the bins was put under fruiting conditions today.
A watering, light and increased aereation (the top bin has four mesh covered holes). The two bins are taped with polythylene wrap to prevent the entry from fruitflies (a big problem at the moment).







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OfflineLarrythescaryrexS
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Re: casing the Panaeolus substrate (pics) [Re: Una]
    #392339 - 09/12/01 02:33 PM (15 years, 9 months ago)

what was used for the humidification?

larry

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OfflineParafreak
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Re: casing the Panaeolus substrate (pics) [Re: Una]
    #394397 - 09/14/01 04:57 PM (15 years, 9 months ago)

Wicked set-up and wicked post Una,

Just curious about your casing recipe, you seem to know what you're doing.

What do you normally use and yhow do you prepare it?

Thanks



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: casing the Panaeolus substrate (pics) [Re: Parafreak]
    #394460 - 09/14/01 06:02 PM (15 years, 9 months ago)

from our website:

Recipe and Preparation of the casing soil

We use the following recipe:

10 parts of peat
5 parts of vermiculite
2 parts of limestone (Marl)


The ingredients are mixed in dry form and while stirring water is added. The amount of water of course depends on the moisture content of the peat. The object is to get as much water in the casing soil as possible without turning it into mud. If the casing gets too wet just add a little more dry ingredients. This casing soil is then filled into oven bags (made of nylon), autoclave bags (PP) or jars and these are sterilised for one hour in the pressure cooker. When the soil has cooled down to room temperature it's ready to use.



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OfflineCableKiller
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Re: casing the Panaeolus substrate (pics) [Re: Una]
    #396319 - 09/17/01 12:54 AM (15 years, 9 months ago)

Una,

How was proper humidification achieved?

CK





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