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Invisiblemobyd
mad agar worker
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Registered: 03/27/02
Posts: 346
Test the potency??
    #3802967 - 02/19/05 10:45 AM (19 years, 1 month ago)

Wanted to ask if somebody knows how to check the potency.
Would like to try different substrates and will find out how the potency varied.

Do you really need a Gas chromatography?

Only for researches....


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Offlinetriptamine
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Registered: 02/06/02
Posts: 73
Loc: SW
Last seen: 23 hours, 32 minutes
Re: Test the potency?? [Re: mobyd]
    #3803981 - 02/19/05 05:02 PM (19 years, 1 month ago)

Do a search for C.B. Gold - he published a fairly simple way to test potency using DMAB as an indicator for psilocin/cybin.

Triptamine

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Invisiblemobyd
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Registered: 03/27/02
Posts: 346
Re: Test the potency?? [Re: triptamine]
    #3805904 - 02/20/05 04:38 AM (19 years, 1 month ago)

Thanks

I found it if anyone is interested
leda The Measure of the Mushroom
EROWID The Measure of the shrooms


The only thing you need is:
p-dimethylaminobenzaIdehyde,
aqueous ferric chloride solution,
Sulfuric Acid,
glacial acetic acid,
Acetic Acid and a
spectrometer.

Oh so easy  :confused:. Not sure if i can afford this?!?


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Edited by mobyd (02/20/05 05:03 AM)

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Offlineshirley knott
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Registered: 11/11/02
Posts: 9,105
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Re: Test the potency?? [Re: mobyd]
    #3806941 - 02/20/05 02:15 PM (19 years, 1 month ago)

i can send you some acetic acid to get you started  :strokebeard: :yesnod:


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buh

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Invisiblemobyd
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Registered: 03/27/02
Posts: 346
Re: Test the potency?? [Re: shirley knott]
    #3806959 - 02/20/05 02:21 PM (19 years, 1 month ago)

Cool, anyone send me a spectrometer??


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Offlinetriptamine
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Re: Test the potency?? [Re: mobyd]
    #3809921 - 02/21/05 07:14 AM (19 years, 1 month ago)

Check ebay...often old ones can be had for <$50.

Triptamine

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OfflineChuangTzu
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Re: Test the potency?? [Re: shirley knott]
    #3835885 - 02/26/05 05:31 AM (19 years, 1 month ago)

You really have access to glacial acetic acid? And you're willing to send it in the mail?

eek

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OfflineChuangTzu
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Re: Test the potency?? [Re: mobyd]
    #3835919 - 02/26/05 06:00 AM (19 years, 1 month ago)

The article calls for a spectroPHOTOmeter or a colorimeter. A decent, working, calibrated, spectrophotometer with bulbs will probably be fairly costly. (The lab I work in just bought a new one for $3000). There are some old pieces of junk on eBay being sold by people who know nothing about them but if you're not experienced with them then getting them working and calibrated might turn out to be more costly than you imagined -- these are sensitive instruments. I use an old one nearly every day and getting it connected to and communicating with a computer is a bitch which easily takes more time than the actual measurement.

Colorimeters can be had for cheap but you need one that can operate at 570nm. It might end up being easier, although still a hassle, to use a published method to extract the alkaloids and then weigh them. The procedure should give a yield, which you can use to correct for the amount lost during extraction.

Good luck.

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Offlineshirley knott
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Re: Test the potency?? [Re: ChuangTzu]
    #3836041 - 02/26/05 07:48 AM (19 years, 1 month ago)

i was just gonna send some vinegar in a McDonalds sachet


--------------------
buh

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OfflineJoops
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Registered: 09/26/04
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Re: Test the potency?? [Re: shirley knott]
    #3843208 - 02/27/05 07:25 PM (19 years, 1 month ago)

We have a mass spectrometer at the university in my department, It costs $700,000.

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