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Invisibleagar
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Didn't pasturize, didn't sterilize & we will see.
    #3797303 - 02/18/05 08:21 AM (11 years, 9 months ago)

I'm posting these here, because they should all turn into a contam farm.

Which, I think they will, but maybe - just maybe ......not.

We will just have to wait & see, day by day & I will post update pic's.

If not, I discovered two (2) differing things. An Innovative cake & bulk substrate with optimal growth nutrients & a inventive method of preparing it.

They were knocked up the other day & have began to colonize cleanly.



UPDATE: (still going strong & no contam's)



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Edited by agar (02/24/05 07:15 AM)


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OfflineSunshinePounder
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3807736 - 02/20/05 07:15 PM (11 years, 9 months ago)

Very cool. :thumbup:


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: SunshinePounder]
    #3809359 - 02/21/05 02:46 AM (11 years, 9 months ago)

2/20 UPDATE...going stronger & no contams :thumbup:


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3809633 - 02/21/05 04:19 AM (11 years, 9 months ago)



This is looking so good, I decided to whip up a bigger batch to test run out. The white you see is condensation, not myc. These have not been knocked up - yet.


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Offlineshroommachine
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3812306 - 02/21/05 06:49 PM (11 years, 9 months ago)

whats in the jars?

Did you do multi-spore, karo, or grain?


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: shroommachine]
    #3812807 - 02/21/05 08:15 PM (11 years, 9 months ago)

I have used all three (edit to add in individual containers), colonized WBS, Liquid Culture & MultiSpore in this test series.


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Edited by agar (02/21/05 08:29 PM)


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3814916 - 02/22/05 02:29 AM (11 years, 9 months ago)

UPDATE 2/21
80% :thumbup: 80%:thumbup: 80%:thumbup:
Well, we are on the downhill side.


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Invisiblewoodzmun
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3815974 - 02/22/05 10:11 AM (11 years, 9 months ago)

All down hill now. :thumbup:


Edited by woodzmun (02/23/05 06:21 PM)


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3820882 - 02/23/05 04:29 AM (11 years, 9 months ago)


About 90% colonized. Seems to be about 10% a day colonization rate.


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OfflineIGnosticAbhorI
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3824142 - 02/23/05 09:26 PM (11 years, 9 months ago)

Looking sweet, I was jus curious, what's multi-spore? I probably know what it is but not familar with the term. O and those jars look strange, i've noticed that Pf cakes and grain cakes look different. Or maybe it's jus my cakes, my pf's always looked like that whereas my wbs cakes were much of a brighter white and had rhizomorphs. Hmmm, just an observation, Gl, looking nice :thumbup:


-Gnostic


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InvisibleHolydiver
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3824158 - 02/23/05 09:29 PM (11 years, 9 months ago)

Quote:

IGnosticAbhorI said:
I was jus curious, what's multi-spore?
-Gnostic




A spore syringe would be multispore.


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OfflineIGnosticAbhorI
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: Holydiver]
    #3824715 - 02/23/05 11:19 PM (11 years, 9 months ago)

Hm, why is that considered multi-spore? Because it has millions and millions of spores? :smirk:

Well what exactly is an isolated strain? I thought that it had something to do with cloning and using agar...

-Gnostic


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OfflineIGnosticAbhorI
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3824724 - 02/23/05 11:20 PM (11 years, 9 months ago)

Sorry agar, don't mean to jack, just curious, wanna make sure that i'm able to follow what's going on :thumbup:

-Gnostic


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3824955 - 02/24/05 12:06 AM (11 years, 9 months ago)

Isolate is healthy myc from agar plate (into spawn jar, or LC), or shroom tissue (clone).

MultiSpore is syringe load of spores, which have to germinate, mate & grow.

Isolate, or clone has done that already & is from prime growth or shroom.

Multispore is like gang-bang. Never know what the babies might be like, other than general strain charicteristics


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: IGnosticAbhorI]
    #3825189 - 02/24/05 12:41 AM (11 years, 9 months ago)



Well.... we are about there & it took a week. Others are a few % behind. Going to give them a day or 3 more, to make sure all are at 110% colonization, then go from there.

Think I will shred one or 2 in a sanitized food processor & spawn into bulk of same substrate.

Others, I suppose I will fruit out as cakes.


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Offlinepenlight438094
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3825207 - 02/24/05 12:44 AM (11 years, 9 months ago)

so whats the sub, or is a mystery untill the flush


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Invisibleagar
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: penlight438094]
    #3826105 - 02/24/05 04:59 AM (11 years, 9 months ago)

Hint - Cellulose, hemicellulose, lignin, fiber, carbohydrates, proteins, oils, lipids, polymers, vitamins, nitrogen, soluble macro mineral trace elements, and cultured (dead) cells of certain microbes, mixed into a holding material that has a high moisture & nutriant retention capacity, as well as good permeability to gas/air.

In other words, Sub will remain a mystery for awhile, as well as how it was pre-treated - prior to being innoc'ed.


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Edited by agar (02/24/05 07:26 AM)


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Offlinecb9fl
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: agar]
    #3826725 - 02/24/05 11:49 AM (11 years, 9 months ago)

Sounds like some kind of protein bar from a health food store mixed with cotton.


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OfflineMAIA
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: cb9fl]
    #3827328 - 02/24/05 02:32 PM (11 years, 9 months ago)

Looking fine.
Good luck !

MAIA


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InvisibleNeedMoreSleep
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Re: Didn't pasturize, didn't sterilize & we will see. [Re: MAIA]
    #3849464 - 03/01/05 12:08 AM (11 years, 9 months ago)

5 days since last update... did you birth them suckahs yet? make ur casings?  update please... lol :grin:

edit: sorry MAIA, was directed at agar :stoned:


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Edited by NeedMoreSleep (03/01/05 12:09 AM)


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Amazon Shop for: ½ Pint Jars, Agar, Wild Bird Seed

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