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Invisiblephrozendata
Carpal Tunnel

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Registered: 04/24/00
Posts: 5,015
Two good questions
    #379279 - 08/26/01 03:29 PM (15 years, 10 months ago)

Shroom Wizards tek says that that two peice lids (ring and disc) can be tightned up before sterlization. Is this true? Would this not stom steam from getting to the substrate?

Also, my friend made a believe to be contaminated syringe from a print from the FSR and he is wonder if he can still save his strain. Any idea?



--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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Offlinetort
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Registered: 06/04/01
Posts: 101
Loc: AMERICA
Last seen: 15 years, 9 months
Re: Two good questions [Re: phrozendata]
    #379309 - 08/26/01 04:19 PM (15 years, 10 months ago)

NO

give me head till i'm dead !


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[red]GOD[/red] [white]BLESS[/white] [blue]AMERICA[/blue]


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Offline777D777
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Registered: 08/19/01
Posts: 182
Loc: The Universe
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Re: Two good questions [Re: phrozendata]
    #379317 - 08/26/01 04:37 PM (15 years, 10 months ago)

Yes you tighten the jars firmely before sterilization. They are self sealing lids becaue the plastic under the rings expands when heated. No ideas on saving the strain though. sorry 8)

>Would this not stom steam from getting to the substrate?
i don't get the question

_______

Eat shrooms and trip life away . . .
In my dream I wish to stay . . .
Smoke bud and live life high . . .
These, my friends, are words to live by!

- Dan the Mushroom Man (?nima)


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: Two good questions [Re: phrozendata]
    #379342 - 08/26/01 05:16 PM (15 years, 10 months ago)

It might stop the steam but it won't stop the heat. Heat is what sterilizes, not steam.
Agar work can save a dirty syringe, print, etceteras.



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Offline777D777
member
Registered: 08/19/01
Posts: 182
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Re: Two good questions [Re: puscle]
    #379348 - 08/26/01 05:31 PM (15 years, 10 months ago)

A good site to learn how to make your own agar for petri dishes is:

http://www.kitchenculturekit.com/Mushroom/agarplates.htm


_______

Eat shrooms and trip life away . . .
In my dream I wish to stay . . .
Smoke bud and live life high . . .
These, my friends, are words to live by!

- Dan the Mushroom Man (?nima)

Edited by 777D777 on 08/26/01 04:39 PM.



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