couple questions about agar - Advanced Mycology

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CLuB99
lost somewhere in time and space

Registered: 10/26/99
Posts: 1,316
Loc: my mind

couple questions about agar

#379094 - 08/26/01 09:26 AM (23 years, 1 month ago)

1. after pouring the agar in the plates, can i use immediately or i should wait a couple days to let is dry a bit??

2. your agar shrinks?? mine yes, after cooling almost 2-3 millimeters.

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dimitri211
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Registered: 04/27/01
Posts: 2,248

Re: couple questions about agar

[Re: CLuB99]
#379102 - 08/26/01 09:44 AM (23 years, 1 month ago)

1. after pouring the agar in the plates, can i use immediately or i should wait a couple days to let is dry a bit??

After the agar solidifies yes you can go right into using it.
If you are fairly new to agar I recommend you pour it and let it sit for 3 days to make sure you did not contaminate it.

2. your agar shrinks?? mine yes, after cooling almost 2-3 millimeters

When agar starts to solidify it will tighten up and shrink a bit. If it starts to pull away from the sides of petri plate then you are not using enough h2o

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Edited by dimitri211 on 08/26/01 10:48 AM.

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Azure
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Registered: 12/31/98
Posts: 469
Loc: California, USA
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Re: couple questions about agar

[Re: CLuB99]
#379122 - 08/26/01 10:26 AM (23 years, 1 month ago)

If you're storing petri dishes, make sure to seal them with parafilm or whatever type of tape you can find. Keep them up-side-down so that the water that condenses won't cause problems.
When making your mixtures, you want to use about 3% agar at the most. I use 11 grams per 400 ml water.

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Anno
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Registered: 06/17/99
Posts: 24,167
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Re: couple questions about agar

[Re: Azure]
#379127 - 08/26/01 10:34 AM (23 years, 1 month ago)

The amount Stamets uses is 20 g per liter, I use from 15-20 g per liter, depending on agar grade and the recipe.
Normaly it shouldn?t shrink after pouring, but it shrinks when it dries out much.

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CLuB99
lost somewhere in time and space

Registered: 10/26/99
Posts: 1,316
Loc: my mind

Re: couple questions about agar

[Re: dimitri211]
#379152 - 08/26/01 11:26 AM (23 years, 1 month ago)

i'm using peroxidated agar, 8 grams each 500ml.
also i noted that right after it solidifies some water is "squeezed" from the agar.
another question: when liquified, you agar have a "fish" smell?
i think i got very low grade agar, maybe i should try another source.

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Anno
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Registered: 06/17/99
Posts: 24,167
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Re: couple questions about agar

[Re: CLuB99]
#379195 - 08/26/01 12:02 PM (23 years, 1 month ago)

Fish smell, ha? Well it deffinitely has a smell, but if this can be described as fish, ... I don?t know.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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