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 CLuB99
lost somewhere in time andspace
 
Registered: 10/27/99
Posts: 1,314
Loc: my mind
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 couple questions about agar 
#379094 - 08/26/01 11:26 AM (16 years, 1 month ago) |
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1. after pouring the agar in the plates, can i use immediately or i should wait a couple days to let is dry a bit??
2. your agar shrinks?? mine yes, after cooling almost 2-3 millimeters.
IL_FUNGO_SACRO la coltivazione, gli enteogeni, in italiano
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dimitri211
Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
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Re: couple questions about agar [Re: CLuB99]
#379102 - 08/26/01 11:44 AM (16 years, 1 month ago) |
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1. after pouring the agar in the plates, can i use immediately or i should wait a couple days to let is dry a bit??
After the agar solidifies yes you can go right into using it.
If you are fairly new to agar I recommend you pour it and let it sit for 3 days to make sure you did not contaminate it.
2. your agar shrinks?? mine yes, after cooling almost 2-3 millimeters
When agar starts to solidify it will tighten up and shrink a bit. If it starts to pull away from the sides of petri plate then you are not using enough h2o
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Want great service,clean spores,fast delivery,lilshop is the place 2 beEdited by dimitri211 on 08/26/01 10:48 AM.
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 Azure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 15 years, 4 months
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Re: couple questions about agar [Re: CLuB99]
#379122 - 08/26/01 12:26 PM (16 years, 1 month ago) |
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If you're storing petri dishes, make sure to seal them with parafilm or whatever type of tape you can find. Keep them up-side-down so that the water that condenses won't cause problems.
When making your mixtures, you want to use about 3% agar at the most. I use 11 grams per 400 ml water.
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Anno
Experimenter
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 26 days
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Re: couple questions about agar [Re: Azure]
#379127 - 08/26/01 12:34 PM (16 years, 1 month ago) |
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The amount Stamets uses is 20 g per liter, I use from 15-20 g per liter, depending on agar grade and the recipe.
Normaly it shouldn?t shrink after pouring, but it shrinks when it dries out much.
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CLuB99
lost somewhere in time andspace
Registered: 10/27/99
Posts: 1,314
Loc: my mind
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Re: couple questions about agar [Re: dimitri211]
#379152 - 08/26/01 01:26 PM (16 years, 1 month ago) |
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i'm using peroxidated agar, 8 grams each 500ml.
also i noted that right after it solidifies some water is "squeezed" from the agar.
another question: when liquified, you agar have a "fish" smell?
i think i got very low grade agar, maybe i should try another source.
IL_FUNGO_SACRO la coltivazione, gli enteogeni, in italiano
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Anno
Experimenter
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 26 days
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Re: couple questions about agar [Re: CLuB99]
#379195 - 08/26/01 02:02 PM (16 years, 1 month ago) |
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Fish smell, ha? Well it deffinitely has a smell, but if this can be described as fish, ... I don?t know.
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