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InvisibleCLuB99
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couple questions about agar
    #379094 - 08/26/01 11:26 AM (15 years, 3 months ago)

1. after pouring the agar in the plates, can i use immediately or i should wait a couple days to let is dry a bit??

2. your agar shrinks?? mine yes, after cooling almost 2-3 millimeters.

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Invisibledimitri211
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Re: couple questions about agar [Re: CLuB99]
    #379102 - 08/26/01 11:44 AM (15 years, 3 months ago)

1. after pouring the agar in the plates, can i use immediately or i should wait a couple days to let is dry a bit??

After the agar solidifies yes you can go right into using it.
If you are fairly new to agar I recommend you pour it and let it sit for 3 days to make sure you did not contaminate it.

2. your agar shrinks?? mine yes, after cooling almost 2-3 millimeters

When agar starts to solidify it will tighten up and shrink a bit. If it starts to pull away from the sides of petri plate then you are not using enough h2o



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Edited by dimitri211 on 08/26/01 10:48 AM.



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OfflineAzure
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Re: couple questions about agar [Re: CLuB99]
    #379122 - 08/26/01 12:26 PM (15 years, 3 months ago)

If you're storing petri dishes, make sure to seal them with parafilm or whatever type of tape you can find. Keep them up-side-down so that the water that condenses won't cause problems.
When making your mixtures, you want to use about 3% agar at the most. I use 11 grams per 400 ml water.



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OfflineAnnoA
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Re: couple questions about agar [Re: Azure]
    #379127 - 08/26/01 12:34 PM (15 years, 3 months ago)

The amount Stamets uses is 20 g per liter, I use from 15-20 g per liter, depending on agar grade and the recipe.
Normaly it shouldn?t shrink after pouring, but it shrinks when it dries out much.

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InvisibleCLuB99
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Re: couple questions about agar [Re: dimitri211]
    #379152 - 08/26/01 01:26 PM (15 years, 3 months ago)

i'm using peroxidated agar, 8 grams each 500ml.
also i noted that right after it solidifies some water is "squeezed" from the agar.
another question: when liquified, you agar have a "fish" smell?
i think i got very low grade agar, maybe i should try another source.

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OfflineAnnoA
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Re: couple questions about agar [Re: CLuB99]
    #379195 - 08/26/01 02:02 PM (15 years, 3 months ago)

Fish smell, ha? Well it deffinitely has a smell, but if this can be described as fish, ... I don?t know.

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