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OfflineNextKind
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Registered: 08/03/04
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Would honey-water multiply your spore supply?
    #3789871 - 02/16/05 05:34 PM (11 years, 11 months ago)

If you only inject 1-2cc into each honey-water jar, and it grows myc.. how much myc do you have put into each jar of substrate? Only a little from each honey water jar?


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InvisibleHolydiver
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Re: Would honey-water multiply your spore supply? [Re: NextKind]
    #3789894 - 02/16/05 05:38 PM (11 years, 11 months ago)

Just follow your typical inoculation guidelines as you would with spore water. Ex: .25, .50 cc's each injection hole, per tek of course.


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To find a place to live between the negatives and positives.


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Invisibletahoe
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Re: Would honey-water multiply your spore supply? [Re: Holydiver]
    #3790065 - 02/16/05 06:16 PM (11 years, 11 months ago)

1/4cc in karo and then go to town on the jars


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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OfflineMontyRev
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Re: Would honey-water multiply your spore supply? [Re: tahoe]
    #3790356 - 02/16/05 07:08 PM (11 years, 11 months ago)

bump*
i wanna know this answer also


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Offlinescatmanrav
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Re: Would honey-water multiply your spore supply? [Re: MontyRev]
    #3790419 - 02/16/05 07:20 PM (11 years, 11 months ago)

Quote:

MontyRev said:
bump*
i wanna know this answer also




The answer was given, you use the same amount of liquid culture as you would spore solution. Like 1cc per jar.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleMrMaddHatter
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Re: Would honey-water multiply your spore supply? [Re: scatmanrav]
    #3791760 - 02/17/05 05:25 AM (11 years, 11 months ago)

Mmmmmmmmm.... honey water and spores. Mmmmmmmmmm..


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Invisibleagar
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Re: Would honey-water multiply your spore supply? [Re: NextKind]
    #3791859 - 02/17/05 06:13 AM (11 years, 11 months ago)

Quote:

NextKind said:
If you only inject 1-2cc into each honey-water jar, and it grows myc.. how much myc do you have put into each jar of substrate? Only a little from each honey water jar?




To start a liquid culture (LC) from spores, you just want to MAKE SURE you inject a few, or more spores into the liquid in each container. It is not a matter of how many cc/ml of fluid you inject, it is a matter of just being sure some spores get into the solution.

Depending on the type jar you use, it's size & if you rig it with a filter, to it can breath a bit, plus something like a few cracked in half marbles to help agitate & CUT UP THE RESULTING MYC MASS into tiny bits.

you will find the mycelia mass is stringy. So much so, it can sometimes plug a syringe needle when you try to extract it. So before you extract any, you want to agitate the jar content - VERY WELL.

If not & you do get a syringe full. It is sort of like a syringe full of FINE SHORT THREADS. If you inject a syringe like that into a verm/brf type jar, ot a grain / wbs jar. You will generaly find most of the myc you inject, will stay on top, or near the top of the jar content. Simply because short thread/like myc will not travel in solution, like spores WILL.

SO..... (IMHO) you want to inject MORE MYC SOLUTION, than you would spore solution. How much more is on you to decide. For quarts of rye/wbs my foaf injects min. 2.5 cc/ml per jar. Then, shakes it gently a few times (right then) to insure myc is spread throught the jar. Instead of just near the top of the jar content.

This will result in colonization a bit more difficult to SEE on the jar sides, but will result in FASTER over/all colonization with liquid culture injection.

Having a stip plate / stir bar setup really helps out. Because you get THICK MYC & can stir it at very hugh RPM, which breaks the myc into tiny tiny threads, instead of long ones.

Hope this helps.



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Offline1uvu
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Registered: 06/11/03
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Re: Would honey-water multiply your spore supply? [Re: agar]
    #3793488 - 02/17/05 03:24 PM (11 years, 11 months ago)

where would i find the tek for honeywater?


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Offlinemidway
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Registered: 02/20/04
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Re: Would honey-water multiply your spore supply? [Re: 1uvu]
    #3793648 - 02/17/05 04:05 PM (11 years, 11 months ago)

Really its also worth saying that liquid cultures are somewhat prone to contaminations, so be extra careful if you are a beginner.
The way i stockpiled spores is i carefully grew out my first syringes in pf jars and printed every cap that i could...now i have way more than i need to play around with. :smile: :smile: :smile: :smile:


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OfflineNextKind
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Registered: 08/03/04
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Last seen: 11 years, 9 months
Re: Would honey-water multiply your spore supply? [Re: midway]
    #3793969 - 02/17/05 05:29 PM (11 years, 11 months ago)

Quote:

midway said:
Really its also worth saying that liquid cultures are somewhat prone to contaminations, so be extra careful if you are a beginner.
The way i stockpiled spores is i carefully grew out my first syringes in pf jars and printed every cap that i could...now i have way more than i need to play around with. :smile: :smile: :smile: :smile:



Was it a chain store than you bought your syringes from? like walgreens? I've found some at a feed store but the needles are very short


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Offlinemidway
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Re: Would honey-water multiply your spore supply? [Re: NextKind]
    #3798935 - 02/18/05 04:41 PM (11 years, 10 months ago)

I got them from a feed store...it was the only way to get large gauge...had to settle for the short needles though.


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