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Offlinebrewwhaha1
Stranger thanmost
Registered: 02/13/05
Posts: 253
Last seen: 11 years, 11 months
syringe inoculation
    #3783105 - 02/15/05 01:15 PM (12 years, 9 months ago)

Is it necessary to heat the syringe needle or is an alcohol bath sufficient for sterilization? Or is it best to do both?


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Offlinethenewguy05
The Mushroom Man
 User Gallery

Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 4 years, 4 months
Re: syringe inoculation [Re: brewwhaha1]
    #3783116 - 02/15/05 01:20 PM (12 years, 9 months ago)

i have used a cotton swab or paper towel with some alchohol on it and i have no problem


Edited by thenewguy05 (02/15/05 01:22 PM)


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InvisibleDamn_Skippy
Fell off theWagon

Registered: 01/25/05
Posts: 403
Re: syringe inoculation [Re: thenewguy05]
    #3783294 - 02/15/05 02:19 PM (12 years, 9 months ago)

heat it... all the lab work I have done in my entire life I used a bunson burner and sometimes alcohol on top of that. Why skip a step that takes you 2 minutes to do, and could save you a lot od headache in the end


--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."


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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: syringe inoculation [Re: Damn_Skippy]
    #3783512 - 02/15/05 03:07 PM (12 years, 9 months ago)

yes you need to heat it. reason being that swabbing it with alcohol will only kill contams on the outside of the needle... remember its hollow, so the flame will kill anything sitting inside the needle too..


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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OfflineShroomGuy86
Shroooooooooooooooom

Registered: 02/06/05
Posts: 1,048
Loc: MycVille
Last seen: 12 years, 6 months
- [Re: NeedMoreSleep]
    #3783553 - 02/15/05 03:14 PM (12 years, 9 months ago)

-


--------------------
"I put Lamborghini doors on that Es-co-lade
Low pro so low look like I'm riding on blades" - 50 Cent




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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: syringe inoculation [Re: ShroomGuy86]
    #3783592 - 02/15/05 03:22 PM (12 years, 9 months ago)

thats the way to do it :thumbup: :thumbup:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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