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Offlinepatzee
enthusiast

Registered: 05/26/01
Posts: 233
Last seen: 11 years, 8 months
50/50+casing/green tricho again!
    #378011 - 08/24/01 08:34 PM (15 years, 3 months ago)

This is my 4th casing try. I've read casing for dummies and lots of other casing advice here.
I autoclave 50/50+ casing in a filter patch bag. I sterilize the foil containers
I put the substrate in a bag and crumble.. so my hands don't touch it.
I wear gloves and mask, use a sterile spoon. I cover it with foil, poke a few small holes. .
I keep it in the dark.I stopped opening and
cking.(used to) I don't spray during colonization and overwater.( did that before)
I keep a hepa filter running 24/7. I finally get mycelial
growth into the casing.. put in chamber/grow light-12hrs. Humidity 85-92%kept with
small pan of perlite with H202in water. And hand spraying same.
Temp 76-80.
I spray lysol-everytime- before lifting lid. I wear a mask.
I fan-5-6xday. And I see green again. I sprayed it down with H202 straight, not diluted.
Now there's no mycelium to be seen in the casing layer at all. The h202 prolly killed it or
atleast set it back. I thought the lime was so supposed to be so great in keeping
the contams down. The 50/50+ also seems to look like a rock. I don't want to spray too much
because of fear of making it crust over.( did that before too and then I had to deep scratch,
which didn't do a thing and I lost the casing) When it seemed dry- I sprayed with h20
then I got green..it's yellowish green.. from what I've read..this is
the green trichoderma mold.This time, I didn't spray even when it looked dry and I still
got the green shit.
I'm so sick of this. I see all these great yields and everyone
saying casing casing..is the way to go. Disgusted newbie. Help please.




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OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 7 months
Re: 50/50+casing/green tricho again! [Re: patzee]
    #378478 - 08/25/01 01:27 PM (15 years, 3 months ago)

Patzee-you're not alone. Even us very experienced cultivators are baffled from time to time with contamination. My suggestion is to work backwards-just use pure vermiculite. With less available nutrients in the casing layer, you don't have much of a chance of contamination. Also, remember that stagnant air and high CO2 levels gives these contaminants a head start over the desired mycelium.
Furthermore, you don't want to sterilize your casing layer because you're killing some beneficial bacteria that helps the mycelium fight off contaminants. If you're not using just pure vermiculite, pasteurize the casing material it in your oven at 180-200 degrees F. for about 1.5 hrs.
If using vermiculite, just sterilize it.



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OfflineMordor
cyborg savage

Registered: 05/15/01
Posts: 118
Loc: North
Last seen: 13 years, 3 months
Re: 50/50+casing/green tricho again! [Re: Azure]
    #378510 - 08/25/01 02:23 PM (15 years, 3 months ago)

You are definitely not alone. We tried 50/50 four times - each one lost to contams. We finally said F*** this shit. Went to Coco Fiber. Works like a dream, no contams since. Don't have to pasturize either. I believe it is the best casing material out there. If you can find it, give it a try, you won't be dissappointed.

It's life. The dark that binds you. - InFlames.


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It's life. The dark that binds you. - InFlames.


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Offlinepatzee
enthusiast

Registered: 05/26/01
Posts: 233
Last seen: 11 years, 8 months
Re: 50/50+casing/green tricho again! [Re: Mordor]
    #378584 - 08/25/01 04:04 PM (15 years, 3 months ago)

Thanks Modor and Azure. ( I had to spray the green shit away again today)
I've read many a post about coco fiber. I don't remember if I show a vendor.
If you have a place to buy from, could you post? Those coconuts can travel
a thousand miles in the ocean and not decay. Makes sense. Try again.



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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: 50/50+casing/green tricho again! [Re: patzee]
    #378900 - 08/26/01 02:12 AM (15 years, 3 months ago)

Doesnt extra lime help fight trich off casing?



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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 17 days
Re: 50/50+casing/green tricho again! [Re: Azure]
    #379061 - 08/26/01 09:33 AM (15 years, 3 months ago)

> My suggestion is to work backwards-just use pure vermiculite.
Yes, diffeinitely buy a different brand of your casing materials.
I use pasteurized coco coir with great success.

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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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