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OfflineRaoul_Duke
Stranger
Registered: 05/31/04
Posts: 22
Loc: San Diego, CA
Last seen: 17 years, 5 months
Primordia: Light-induced or not?
    #3778236 - 02/14/05 12:39 PM (17 years, 5 months ago)

This morning, I checked on my cakes that were inoculated following the PF Tek almost to a "T." The only change I made from the Tek was to keep them at around 82 degrees with roughly 95 percent humidity. The colonization exploded initially and has been doing well ever since. I figured, however, that I'd be seeing the beginnings of primordia at this point.

Some of my jars aren't quite ready for birthing--I can see that the colonization isn't quite complete. The rest, however, look good, but again, I don't see any primordia. Hence my question: do the jars need exposed to light before the primordia will appear? I've been keeping the jars in near total darkness for their colonization.

Any help would be appreciated. I'm itching to birth (and dunk) these guys but don't want to move too soon.

Thanks,

RD


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InvisibleButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
Re: Primordia: Light-induced or not? [Re: Raoul_Duke]
    #3778265 - 02/14/05 12:43 PM (17 years, 5 months ago)

just something to remember,even though the jars apear fully colonized,they may nut be 100% colonized all the way thru.Also some strains just take longer than others.i usually wait untill they apear 100% colonized,then 3-5 days later just to be sure,then i dunk,and birth.Never a problem this way.


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Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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OfflineSpaceVox
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Registered: 01/22/05
Posts: 622
Loc: Netherlands
Last seen: 10 months, 28 days
Re: Primordia: Light-induced or not? [Re: ButterNut]
    #3778283 - 02/14/05 12:49 PM (17 years, 5 months ago)

Do you keep the humidity 95% at incubation??:S


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OfflineKingBahamut
visiting goon

Registered: 11/16/04
Posts: 56
Last seen: 17 years, 5 months
Re: Primordia: Light-induced or not? [Re: ButterNut]
    #3778288 - 02/14/05 12:49 PM (17 years, 5 months ago)

you don't want to see primordia until you get the things into your fruiting chamber. those mushrooms you see growing inside the jars on some people's teks are a bad thing and the result of letting em get too much light while colonizing. wait till they're 100% finished, dunk em, and drop temps. then you'll see primordia in a day or two


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OfflineRaoul_Duke
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Registered: 05/31/04
Posts: 22
Loc: San Diego, CA
Last seen: 17 years, 5 months
Re: Primordia: Light-induced or not? [Re: KingBahamut]
    #3778313 - 02/14/05 12:56 PM (17 years, 5 months ago)

Quote:

you don't want to see primordia until you get the things into your fruiting chamber. those mushrooms you see growing inside the jars on some people's teks are a bad thing and the result of letting em get too much light while colonizing. wait till they're 100% finished, dunk em, and drop temps. then you'll see primordia in a day or two




Right on. I have only checked on them three or four times in three weeks--in other words, I've been careful not to let too much light in during the colonization process. As mentioned, some of the jars look pretty good, so as Butternut suggested, I'll wait another few days and then birth them. I figure a 12-hour dunk and a temperature drop to around 72 degrees should produce a nice first flush.

Thanks for the help.

RD

P.S. Butternut, your squash rules. Perfect for a winter bisque.


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InvisibleMagash
Da Bud Guru
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Registered: 07/25/02
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Re: Primordia: Light-induced or not? [Re: Raoul_Duke]
    #3781021 - 02/14/05 11:46 PM (17 years, 5 months ago)

This may help

I know this belongs in the growlog forum but it seems more would see it here that can use it. That and I?m sure most have seen it already but maby somebody can use this to help them out.

Grow Log
Tek : PF
Strain : South American [SA]
Spores : Came from homemade prints. 36 total jars to be injected.
Substrate :Brown Rice Flour [BRF] and Vermiculite [Verm]
---------------------------------------------------------------------------------------------------
10:15 PM 3/4/03 : Project is started. Jar prep is just putting small holes in the lids for the spore syringe and then taping them closed until inoculation. Tape used is the standard black electrical tape. First batch of jars is mixed. The mix used is 2 parts verm, 1 part water, and 1 part brf. [ This mix is the max fruiting formula by PF.] Mix is put into 1/2 pint canning jars lightly, not packed in, and left it airy. Put substrate in until it reaches the threads of the jar, then add dry verm the rest of the way.
Jars are PCd at 10psi or 240f for a period of 45 minutes. Then jars were left to sit overnight to cool.

<a href="https://files.shroomery.org/files/040103-23/23611-pic_1.jpg" target="_blank"></a>

8:39 PM 3/5/2003 : First batch of jars injected. (24 jars)

10:04 AM 3/6/2003 : Incubator is running to low 77F. Going to turn it up today.

<a href="https://files.shroomery.org/files/040103-23/69842-Pic_2_.jpg" target="_blank"></a>

5:45 PM 3/6/2003 : Second batch of jars is in the PC will be injected tomorrow. Turned up incubator runs at 83F. 86F is the best and if the incubator is 83F and myc growing in the jars is suppose to create a little heat I figure this should work out well.

1:11 PM 3/7/2003 : Second batch of jars is done. (12 jars)

12:10 PM 3/9/2003 : Jars are doing good, no signs of contams

<a href="https://files.shroomery.org/files/040103-23/74102-Pic_3_.jpg" target="_blank"></a>

2:54 AM 3/19/2003 : Tape removed from the holes today and coffee filters put over the top of the jars for added protection. Holes were opened so the CO2 in the jars could escape. I gave 12 jars to a friend who kept fucking up. 24 jar?s about 75%done.

<a href="https://files.shroomery.org/files/040103-23/89166-Pic_4.jpg" target="_blank"></a>

12:13 PM 3/22/2003 : Well it?s Saturday and the jars will probably be done by the 25th. So it was 36 jars. 12 were given to a friend, 24 done by Tuesday.

10:26 PM 3/23/2003 : 15 jars were done enough to put into dunk. I dunk and cold shock before birthing and after every flush. The other 9 jars have a spot the size of a pea that has to be covered so I?ll do those on the 25th.

<a href="https://files.shroomery.org/files/040103-23/81008-pic_5.jpg" target="_blank"></a>

7:17 PM 3/24/2003 : 15 jars put into the pod. Put in and put a layer of moist verm on top. With geolite I just put the cakes on it. With some other chambers I put the cake on a lid filled with verm. This is done to give the shrooms on the bottom and the top of the cake some added moisture.
The other 9 jars just finished so I?m going to give them a couple more days to make sure the middle of the cake is done.

<a href="https://files.shroomery.org/files/040103-23/68648-Pic_6.jpg" target="_blank"></a>

4:57 PM 3/27/2003 : 5 More cakes birthed today, 4 left going to do them tomorrow or 3/29/2003

3:58 PM 3/29/2003 : Well I lost another cake. My wife?s little 1 cake pod made from a 2 liter and a small fish pump and a little geolite used up her last cake so I gave her a SA to grow. The last 3 are in the cold shock dunk.
So the total is 36 jars done all colonized with no contams. 12 given to a friend, 1 to the wife, 23 mine

12:08 PM 3/30/2003 : Last of jars put in

12:02 PM 3/31/2003 : The first 15 cakes are pinning nicely and some have shrooms coming from the bottom.

<a href="https://files.shroomery.org/files/040103-23/38858-Pic_7.jpg" target="_blank"></a>

11:01 AM 4/3/2003 : Things look to be going well.

<a href="https://files.shroomery.org/files/040103-23/55988-Pic_8_.jpg" target="_blank"></a>

1:02 AM 4/4/2003 : Took the first flush from four of the cakes. More will be done later today.

<a href="https://files.shroomery.org/files/040103-23/36336-Pic_9.jpg" target="_blank"></a>

5/8/2003 : Total 23 cakes 164 grams dried.

From what I?ve seen this needs to be up for a little bit.


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
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Re: Primordia: Light-induced or not? [Re: Magash]
    #3783185 - 02/15/05 01:41 PM (17 years, 5 months ago)

"Bump in the night!"


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineRaoul_Duke
Stranger
Registered: 05/31/04
Posts: 22
Loc: San Diego, CA
Last seen: 17 years, 5 months
Re: Primordia: Light-induced or not? [Re: Magash]
    #3783629 - 02/15/05 03:31 PM (17 years, 5 months ago)

Quote:

From what I?ve seen this needs to be up for a little bit.




I agree. I could recite the PK Tek front and back while lit up, but it's these subjective judgments that always get me so wishy-washy. My jars have been incubating for 23 days now and I still find it hard to dunk them, even though everything tells me they should be ready to go. Your growlog helped considerably, however, as I'm using almost the exact same setup and the exact same temperatures, and my jars have been "under" for longer than is listed in your March 2003 log. I think I'll dunk them tonight and birth tomorrow.

Thanks again,

RD


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