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OfflineFluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 13 years, 4 months
spore prints can be contaminated?
    #3740480 - 02/06/05 11:32 AM (19 years, 1 month ago)

Hey I got some spore prints but was rather stupid and made a syringe using the oven tech, which didn't work at all and the syringe has other bacteria spores in it.

My question is now that I opened the spore print, I must assume that it is now fully contaminated? You have to open spore prints in a controlled environment, like a glove box but how come you can store them just in a bag?

Some light on this issue would be nice, thanks.


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ABSTRACT ART (Mine) http://nathanbelomy.com

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OfflineKaptKid
Spaced Pirate
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Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 1 month
Re: spore prints can be contaminated? [Re: Fluxburn]
    #3740490 - 02/06/05 11:40 AM (19 years, 1 month ago)

Your right. Need Flow hood or glove box. Improves chances greatly.

GL


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Child of the 60's, Tripping ever since.


:sun:

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OfflineFluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 13 years, 4 months
Re: spore prints can be contaminated? [Re: KaptKid]
    #3740511 - 02/06/05 11:49 AM (19 years, 1 month ago)

sucks a nut hardcore, I gotta laugh as my agar plates are not contaminated (at least no growth other than white) but my damn syringes get contaminated. I guess the game for not getting the hepa airhood is over. I will have to use a glove box in the mean time to hold me off, since I can't afford it!

someone should make a damn hepa airhood kit, everyone here would buy one if it was $200-300.


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ABSTRACT ART (Mine) http://nathanbelomy.com

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Offlinedelphis
wanderer
Registered: 11/04/04
Posts: 14
Last seen: 18 years, 9 months
Re: spore prints can be contaminated? [Re: Fluxburn]
    #3740626 - 02/06/05 12:46 PM (19 years, 1 month ago)

i got spores from my first contaminated growing experience, i'll try to inoculate them and then'll see what happens..

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Offlineprototypical_man
ass model

Registered: 02/01/05
Posts: 396
Loc: Doral Florida
Last seen: 8 years, 1 month
Re: spore prints can be contaminated? [Re: delphis]
    #3741219 - 02/06/05 03:21 PM (19 years, 1 month ago)

try doing a karo tek with your contamed spores. add a good dose of h2o2 once you see some myc.


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I have 29 Strains as of current.. I LOVE TO TRADE. See my bio for the strains I currently have.

I seek: Strains I don't have, exotic species, Edibles, Vacutainers, A accurate scale, chicken manure, worm castings, worms for making my own worm castings, plastic lids for mason jars, light malt extract, Kelp Meal, A Ph Tester, A microscope.

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OfflineAmygdala
Learner

Registered: 06/30/04
Posts: 158
Last seen: 8 years, 7 months
Re: spore prints can be contaminated? [Re: prototypical_man]
    #3741242 - 02/06/05 03:25 PM (19 years, 1 month ago)

Quote:

prototypical_man said:
try doing a karo tek with your contamed spores.




May I ask why anyone would want to do such a thing?

On a quick sidenote, how is it that you have a trade rating mere hours after your account was created? tsk tsk...


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"Only two things are infinite, the universe and human stupidity, and I'm not sure about the former." --Albert Einstein

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InvisibleLouiseLouise
starstruck
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Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
Re: spore prints can be contaminated? [Re: Amygdala]
    #3741336 - 02/06/05 03:51 PM (19 years, 1 month ago)

Good question.


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"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC

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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
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Re: spore prints can be contaminated? [Re: Fluxburn]
    #3741350 - 02/06/05 03:56 PM (19 years, 1 month ago)

Quote:

Fluxburn said:
someone should make a damn hepa airhood kit, everyone here would buy one if it was $200-300.





one sorta like this


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Offlineorichalc
Stranger

Registered: 01/30/05
Posts: 71
Last seen: 18 years, 1 month
Re: spore prints can be contaminated? [Re: Prisoner#1]
    #3741369 - 02/06/05 04:00 PM (19 years, 1 month ago)

nice

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OfflineAmygdala
Learner

Registered: 06/30/04
Posts: 158
Last seen: 8 years, 7 months
Re: spore prints can be contaminated? [Re: orichalc]
    #3745592 - 02/07/05 01:32 PM (19 years, 1 month ago)

I second that...

NICE  :grin:

prototypicalman... no comment?  :smirk:


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"Only two things are infinite, the universe and human stupidity, and I'm not sure about the former." --Albert Einstein

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OfflineFluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 13 years, 4 months
Re: spore prints can be contaminated? [Re: Amygdala]
    #3777055 - 02/14/05 01:00 AM (19 years, 1 month ago)

how the hell did u make that?


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ABSTRACT ART (Mine) http://nathanbelomy.com

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OfflineVertigo6911
Entheobotanist
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Posts: 1,834
Loc: Netherlands
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Re: spore prints can be contaminated? [Re: Fluxburn]
    #3777129 - 02/14/05 01:31 AM (19 years, 1 month ago)

scuse me but doesent liquid culture exist specificly so u can use
dirty sporeprints?

just make a bunch of plates, some will be contammed some will be clean... no reason to trow out the print yo.


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-Know ye not that ye are gods?-
My homepage

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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 14 years, 11 months
Re: spore prints can be contaminated? [Re: Vertigo6911]
    #3777136 - 02/14/05 01:35 AM (19 years, 1 month ago)

Prisoner did u make or buy that?


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OfflineCyber
Ash
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Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 10 months, 16 days
Re: spore prints can be contaminated? [Re: LengthyFellow]
    #3777525 - 02/14/05 06:16 AM (19 years, 1 month ago)

To clean up a contaminated print you will have to use agar and culture it out. Karo does nothing, the contamination will grow in karo just as good as the spores.

Why spend $200 on a flow hood when you can bild a glove box for $20




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OfflineVertigo6911
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Re: spore prints can be contaminated? [Re: Cyber]
    #3777531 - 02/14/05 06:20 AM (19 years, 1 month ago)

nice glovebox... the thing i wonder about those is how to make sure u dont introduce contams when u place ure equiptment in the chamber...


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-Know ye not that ye are gods?-
My homepage

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OfflineCyber
Ash
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Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 10 months, 16 days
Re: spore prints can be contaminated? [Re: Vertigo6911]
    #3777542 - 02/14/05 06:29 AM (19 years, 1 month ago)

That would depend on how you use it.

I have had my Glove Box hooked up to a hepa filter, In those instances it is a forced air box.

If you are using it with out forced air you will load it, let it set for a while, then use your sterilizing agent (alcohol or lysol) inside the box. Then let it set for a while.

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OfflineVertigo6911
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Re: spore prints can be contaminated? [Re: Cyber]
    #3777559 - 02/14/05 06:45 AM (19 years, 1 month ago)

kewl thxz


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-Know ye not that ye are gods?-
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