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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
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Re: Different Types of Syringes [Re: cuatiklad]
    #3776850 - 02/13/05 11:46 PM (19 years, 1 month ago)

this may be a dumb idea, but i wonder if you could create a liquid culture directly in a 60 ml syringe? you could suck up sterilized culture directly after pc'ing without having to strain, and you would be able to have a couple syringes of mycellium culture, which would prevent cross contamination if there were to be any... i'm thinking just wrapping the needle in surgical tape or something... thoughts???


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Invisibleagar
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Registered: 11/21/04
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Re: Different Types of Syringes [Re: NeedMoreSleep]
    #3776982 - 02/14/05 12:38 AM (19 years, 1 month ago)

You could. Suck up some spores in solution, then suck up some liquid culture nutrient solution until the syringe was (say about) 75% full. Cap off & incubate.

But...... hell. You could also use Classic EvenFlo plastic Baby bottles :eek: They hold a bunch of LC & work well.

All the parts PC, you get 3 for about $3 @ WalMart. You can stick a syringe right through the tiny hole in the nipple. You can also squeeze the nipple shut & shake the holy living shit out of one.


2nd use is..... :wink: cut in 3 parts, they also make good bag necks.


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OfflineFluxburn
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Re: Different Types of Syringes [Re: agar]
    #3777115 - 02/14/05 01:26 AM (19 years, 1 month ago)

man I was going http://www.culturekits.com/

I guess it is more medical based with slightly higher prices, but they have b&d syringes (very nice) and other crap like vacuseal containers.


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OfflineVertigo6911
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Re: Different Types of Syringes [Re: cuatiklad]
    #3777121 - 02/14/05 01:28 AM (19 years, 1 month ago)

i noticed that the same needles fit on both types of syringes....


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Offlinepenlight438094
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Registered: 10/01/04
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Re: Different Types of Syringes [Re: Vertigo6911]
    #3777127 - 02/14/05 01:30 AM (19 years, 1 month ago)

watch me go

you reuse needles but not syringes because you can find syringes but not needles

the large syringe came in contact with with some type of alcohol, probally iso

let me know if i'm right plz


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OfflineVertigo6911
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Re: Different Types of Syringes [Re: penlight438094]
    #3777528 - 02/14/05 06:18 AM (19 years, 1 month ago)

uuhhh the pic was just to illustrate the luer lock/slip and how the needle fits both.
the big one was cleaned with alcohol, boiled and filled with water and the little one is empty and dirty...
as u can see its a crappy connection compared to the large one where it screws in...
for you needle shoppers: get a fat needle.
the thin ones take ages to fill or squirt...


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OfflineCyber
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Re: Different Types of Syringes [Re: penlight438094]
    #3777553 - 02/14/05 06:40 AM (19 years, 1 month ago)

Quote:

penlight438094 said:
you don't need 60 ml if you want a big syringe go for a 20, unless you will be doing several dozen jats at a time




I like to use the 60cc syringe to sterilize water. Then when I make syringes I can may 5 12cc spore syringes at a time.


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