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OfflineWorkmanV
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Results of multispore inoculation, Psilocybe mexicana, Jalisco * 2
    #3774672 - 02/13/05 05:30 PM (11 years, 9 months ago)



Small multispore test tray of Psilocybe mexicana, Jalisco strain. Grass seed cased with peat/calcium carbonate.



Selective harvest of various phenotypes of the above tray. The one on the far right was deemed the best and has been cloned for future work.



Clone isolated and fruited - 1st flush. Note the quality of the mushroom shape, color and spore production.


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Edited by Workman (05/09/05 03:33 PM)


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InvisibleHangnail
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3774695 - 02/13/05 05:36 PM (11 years, 9 months ago)

now that's how it's done. those actually look like mexicana!

good to see you posting so much lately

Biff


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OfflinePsilygirl
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3774782 - 02/13/05 06:03 PM (11 years, 9 months ago)

once again I am very impressed.  keep up the good work, they look beautiful :thumbup:


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InvisibleSemilanceata
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3777182 - 02/14/05 04:05 AM (11 years, 9 months ago)

Very nice!!!  :thumbup: :thumbup:

Hey man, I have some questions  :confused:(if you wan?t to answer them)
What is your grain/water ratio?
How long did you incubate the grain?
Did you cold shock it for a long time?
At what temps are you fruiting them?
Do you think it?s better to start from multispore instead of agar wedges?

Thanks and GL.


--------------------
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OfflineWorkmanV
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Semilanceata]
    #3779173 - 02/14/05 05:33 PM (11 years, 9 months ago)

Grain/water ratio 2:1
Incubated 2 weeks at 75-80F
No cold shock
Fruiting at 75-78F
These were inoculated with agar wedges, but no isolation of strains on the agar was attempted. I personally never use spore syringes.


--------------------
Research funded by the patrons of
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Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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InvisibleWorld Spirit
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3780317 - 02/14/05 09:31 PM (11 years, 9 months ago)

Beautiful!


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OfflineShroomeroUno
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: World Spirit]
    #3780539 - 02/14/05 10:36 PM (11 years, 9 months ago)

Awsome.Great Work.
ShroomeroUno


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OfflineBlue Helix
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: ShroomeroUno]
    #3786398 - 02/16/05 12:01 AM (11 years, 9 months ago)

Great work, Workman.


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InvisibleMrMaddHatter
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3787689 - 02/16/05 03:26 AM (11 years, 9 months ago)

Two thumbs up!  :thumbup: :thumbup:


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Invisibleralphster44
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3789349 - 02/16/05 03:17 PM (11 years, 9 months ago)

WOW.....That is the BEST I have ever seen!!!

My god....that is some great work there!!!

Absoutely wonderful!!!!
Perfect job Workman!!!
Excellent!!!

Holy shit!!! :thumbup: :heart: :mushroom2:


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OfflineCyber
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3789393 - 02/16/05 03:27 PM (11 years, 9 months ago)

I thought you were supposed to wait 3 months before casing Ps. Mexicana.

Only 2 weeks?

Mexicana is a neat mushroom!


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Invisibletahoe
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: ralphster44]
    #3789747 - 02/16/05 05:02 PM (11 years, 9 months ago)

Quote:

ralphster44 said:
WOW.....That is the BEST I have ever seen!!!

My god....that is some great work there!!!

Absoutely wonderful!!!!
Perfect job Workman!!!
Excellent!!!

Holy shit!!! :thumbup: :heart: :mushroom2:



he must not have used a bag


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InvisibleSemilanceata
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3789812 - 02/16/05 05:20 PM (11 years, 9 months ago)

Quote:

Workman said:
These were inoculated with agar wedges, but no isolation of strains on the agar was attempted. I personally never use spore syringes.




So, with psilo mex and maybe other exotic shrooms it?s better to inoc. grain with lots of strains instead of "trying" to isolate a single fruiting strain on agar, right?
You know, I started not long ago with ps.mex and pan cyans cultivation via isolation of strains on agar and the failure is 100%. Everything is healthy but not a single fruit.

Thanks.


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OfflineWorkmanV
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Semilanceata]
    #3798652 - 02/18/05 03:37 PM (11 years, 9 months ago)

This is just what prefer to do when I only have spores, maybe its not ideal but it works for me. If a fresh mushroom is available I prefer a clone for the first attempt.


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Offlinepelon
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3942246 - 03/20/05 01:20 AM (11 years, 8 months ago)

Would you share your agar recipe for use with mexicana? Also how long after a multispore inoculation of the agar do you see growth? Any help would be greatly appreciated.


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OfflineRogerRabbitV
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: pelon]
    #3942336 - 03/20/05 01:54 AM (11 years, 8 months ago)

Very impressive as usual Workman.


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OfflineWorkmanV
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: pelon]
    #3943785 - 03/20/05 01:39 PM (11 years, 8 months ago)

I just use commercial malt agar. I think its 2% malt.

I generally see growth in 4-10 days at 85F. Depends on how good your eyes are.


--------------------
Research funded by the patrons of
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Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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InvisibleOlgualion
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
    #3944000 - 03/20/05 02:39 PM (11 years, 8 months ago)

Impressive indeed!!

Always nice to see your work!


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Mexicana and Tampanensis

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