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OfflineKaptKid
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What are my Chances???
    #3768066 - 02/11/05 08:44 PM (12 years, 19 days ago)

I have a pf cake of Malabar.It only has 4 small shrooms(1 1/4 in caps).The veils broke last night.Just been waiting to make prints.
While I was doing this found a spot of the mean green.Will my prints be contam'ed?


:sun:


--------------------
Child of the 60's, Tripping ever since.


:sun:


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Offlinejc_illenium
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Re: What are my Chances??? [Re: KaptKid]
    #3768110 - 02/11/05 08:53 PM (12 years, 19 days ago)

no


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on my knees and burnin
my piss and moans the fuel that
set my head on fire
smell my soul its burnin


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InvisibleRoadkill
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Re: What are my Chances??? [Re: KaptKid]
    #3768154 - 02/11/05 09:00 PM (12 years, 19 days ago)

Not sure...but you'll find out when you use the prints.

I'd hurry to make those prints.

try cutting out the green...see if the green is running through the inside of the cake...or just the spot on the outside.

good luck!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinejc_illenium
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Re: What are my Chances??? [Re: Roadkill]
    #3768184 - 02/11/05 09:05 PM (12 years, 19 days ago)

1 in a million.


--------------------
on my knees and burnin
my piss and moans the fuel that
set my head on fire
smell my soul its burnin


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OfflineLaughingJim
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Re: What are my Chances??? [Re: KaptKid]
    #3768196 - 02/11/05 09:07 PM (12 years, 19 days ago)

If the contamination is just now being seen, then it may not have had enough time to shoot its spores.

Put syran wrap over the cake, around the mushrooms. When you get done, you should only see the stalks and caps sticking out of the diaper wrapped cake.

The spores should be fine, just don't eat the shrooms.

After you get the spore prints, get a flesh culture going also.

Make a honey-water solution, 80% water and 20% honey. PC that mixture after you mix it well. Throw some jars of water in there also, you can always use sterile water. At the same time you PC, drop the whole cap that has no more spores left to print, into 100% peroxide (Common 3% Peroxide). Throw that cap, that is sitting in the peroxide solution, into the fridge. Let the honey-water cool over night while the cap chills. (You can keep the cap at room temp, if you don't want to put it into your fridge.)

When your honey-water is cool, you can get your cap ready. Bring the cap to your "Clean/Sterile" area. Use a sterile knife to pull the skin back off the cap, so you expose the flesh underneath. Cut a section ot from near the center of the cap, try not to cut so deep that you get to the gills. A thin piece about as thick as a sheet of paper is all that is needed. Transfer that quickly into your honey-water jar and seal it up.

Wipe down the entire jar with an alcohol-dampened paper-towel before you throw it into the incubation chamber. (Seal any holes in the cover if any exist.)

In about two weeks you should have the beginnings of a thick ball of mycelium clone-juice brewing in the bottom of the jar. Shake it every few days to spread it around.

You can suck that spawn up with a super-sterile needle, and inoculate your jars with that, just as you would with spores. (They will also grow twice as fast... In two weeks your jars will be, or will almost be 100% colonized!)

If you see anything other than a thick ball of white, you can "Try" to suck it out with a sterile needle, but that may not work well. You should be safe though. (Most contamination has a harder time trying to grow under water. If you do get contamination, it may all be on the surface, and should be easy to remove.)


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OfflineMachmood
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Re: What are my Chances??? [Re: jc_illenium]
    #3768600 - 02/11/05 10:24 PM (12 years, 19 days ago)

Quote:

jc_illenium said:
1 in a million.




so your saying he has a chance


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OfflineKaptKid
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Re: What are my Chances??? [Re: LaughingJim]
    #3770199 - 02/12/05 07:55 AM (12 years, 19 days ago)

LaughingJim,after relooking the mean green was all over that cake but still white.Hopefuly it had not released its spores,only one small spot green.Cut the base of shrooms,no sign of contam in stem.Don't worry no one will be eating these.Working on a flow hood.Will start working on cultures after it is done.Thanks for your input.Always helpful.

Peace
KaptKid :sun:


--------------------
Child of the 60's, Tripping ever since.


:sun:


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