Alternative to autoclaving for agar? - Mushroom Cultivation

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iudexk
member

Registered: 08/18/01
Posts: 107
Loc: UK
Last seen: 21 years, 8 months

Alternative to autoclaving for agar?

#376425 - 08/22/01 12:57 PM (22 years, 1 month ago)

On the directions for my antibacterial MEA, it sez to mix with water, then autoclave for 45mins @15psi. I do not have an autoclave... can I just add double the amount of water and boiling half of it away to sterilise?

Many thanks,
IudexK

http://www.magickherbs.co.uk/

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puscle
genius of love

Registered: 01/06/01
Posts: 4,539
Loc: NY

Re: Alternative to autoclaving for agar?

[Re: iudexk]
#376429 - 08/22/01 01:06 PM (22 years, 1 month ago)

Nope. Get yourself a pressure cooker for "real" sterilization.

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iudexk
member

Registered: 08/18/01
Posts: 107
Loc: UK
Last seen: 21 years, 8 months

Re: Alternative to autoclaving for agar?

[Re: puscle]
#376432 - 08/22/01 01:12 PM (22 years, 1 month ago)

Wont it make do till then though? It works for making cakes with!

http://www.magickherbs.co.uk/

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puscle
genius of love

Registered: 01/06/01
Posts: 4,539
Loc: NY

Re: Alternative to autoclaving for agar?

[Re: iudexk]
#376434 - 08/22/01 01:14 PM (22 years, 1 month ago)

You may be able to "make do" with doubling the steaming time.

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puscle
genius of love

Registered: 01/06/01
Posts: 4,539
Loc: NY

Re: Alternative to autoclaving for agar?

[Re: puscle]
#376436 - 08/22/01 01:17 PM (22 years, 1 month ago)

Doubling the water will just make it more "soupy".

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iudexk
member

Registered: 08/18/01
Posts: 107
Loc: UK
Last seen: 21 years, 8 months

Re: Alternative to autoclaving for agar?

[Re: puscle]
#376445 - 08/22/01 01:28 PM (22 years, 1 month ago)

No I meant use twice the normal amount of water, and boil half of it off, so u end up wiv the normal amount of water but it would have been thoroughly steamed.

http://www.magickherbs.co.uk/

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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