Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Anno]
    #3892827 - 03/09/05 01:20 PM (19 years, 2 months ago)

lol. i'm sure it is still manufactured, or a related chemical is, if it proved useful (as clearly it does)


--------------------
buh

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: shirley knott]
    #3894872 - 03/09/05 08:20 PM (19 years, 2 months ago)

From looking at that last pic, It looks like you rolled the dry tissue into the agar, thus several points of growth emerging. They appear to be growing together seamlessly, as opposed to forming sectors which is what one would expect if it were spores germinating. It looks to me like the dry tissue has begun to grow again. Is that your take as well?


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: RogerRabbit]
    #3897957 - 03/10/05 01:38 PM (19 years, 2 months ago)

I did streak dried gill tissue over the surface using sterile forceps to spread out the spores and then leave the tissue on the agar at the end of the streak. Typically the tissue mass contaminates but spores left by the streaking usually germinate cleanly. I doubt there is anything but spores producing mycelium but there is no way of knowing. There are several mycelium types growing.

I did get a 10 pound bag of benlate as a lawn fungicide just before it was pulled from the market. It should last awhile using only 10mg amounts at a time.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 8 days
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3903241 - 03/11/05 01:50 PM (19 years, 2 months ago)

If you think there might be a contamination issue. Simply pour another layer of hot agar on top, completely covering the growth you have going. A whole new layer. The mycelium will crawl through to the top of the new layer of agar and will leave behind the contaminates. Then lightly scrape some of the new growth and transfer to a whole new dish. That should clean up your culture.

Edited by hotnutz (03/11/05 01:52 PM)

Extras: Filter Print Post Top
Offlinered914
Minion
Registered: 11/05/04
Posts: 134
Loc: Utah
Last seen: 18 years, 8 months
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Hotnuts]
    #3926536 - 03/16/05 04:24 PM (19 years, 2 months ago)

Hotnutz..
Just how hot would the agar be? sounds like a great plan.

Extras: Filter Print Post Top
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3928811 - 03/17/05 12:40 AM (19 years, 2 months ago)

Quote:

Workman said:
I did streak dried gill tissue over the surface using sterile forceps to spread out the spores and then leave the tissue on the agar at the end of the streak.  Typically the tissue mass contaminates but spores left by the streaking usually germinate cleanly.  I doubt there is anything but spores producing mycelium but there is no way of knowing.  There are several mycelium types growing.




But a clean culture COULD be cultured out of this...........right???. (You would know!!)

I think its time for me to get back on the agar and get some experiments running.

There HAS to be a way to go from dried specimen to clean culture, no matter the contaminates.  :thumbup:

Pure cultures have been cultured from wild prints which have been exposed to any and everything outside, yet are still cultured out. I've done it myself.

Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: MrMaddHatter]
    #3930088 - 03/17/05 11:06 AM (19 years, 2 months ago)

Right, any obvious mycelium can be isolated away from contaminates. Sometimes it takes more than one attempt.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
Offlinered914
Minion
Registered: 11/05/04
Posts: 134
Loc: Utah
Last seen: 18 years, 8 months
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3935090 - 03/18/05 10:25 AM (19 years, 2 months ago)

I tried putting questionable spores in agar, and covering it with two sheets of boiled newspaper after cooling. The mycelium grew out to the outside layer. Peal the outer layer off to a fresh dish of agar. It appears clean. It would appear that contams do not like to be covered. I have also moved to the wide mouth jam jars to get away from the agar quickly drying out as the newspaper wicks off water fast. I tried spores alone in newspaper, but it works for oysters but not cubes, I guess they need more nutes.

Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #4123798 - 05/02/05 02:55 PM (19 years, 21 days ago)

Results of attempted fruiting of above plate on grass seed cased with peat/calcium carbonate.

Day 11 after casing, incubated at 78F




--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery

Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #4123890 - 05/02/05 03:21 PM (19 years, 21 days ago)

Nice!!! :bow:


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #4128899 - 05/03/05 05:35 PM (19 years, 20 days ago)

:thumbup: Very nice work!

Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #4133174 - 05/04/05 02:45 PM (19 years, 19 days ago)

Its coming along.



--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery

Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #4133438 - 05/04/05 03:41 PM (19 years, 19 days ago)

its nice to know that you are working to bring new strains that no one else has to the market


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Offlinexburn
V card
Registered: 03/10/05
Posts: 707
Last seen: 11 years, 10 months
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: tahoe]
    #4163283 - 05/11/05 06:48 PM (19 years, 12 days ago)

why do you use grass seeed?


--------------------
Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.

Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
Trusted Cultivator
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: xburn]
    #4163630 - 05/11/05 08:01 PM (19 years, 12 days ago)

Ah, thats a good question. I use it because it seems to work well with a wide range of species. It is my first choice for specimens with unknown growth parameters.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
Offlinexburn
V card
Registered: 03/10/05
Posts: 707
Last seen: 11 years, 10 months
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: xburn]
    #4165753 - 05/12/05 09:07 AM (19 years, 11 days ago)

oh i see, workman i do wonder how would i get into doing the work that you do. I am facsinated nore by the mycology than the drugs and stuff.


--------------------
Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.

Extras: Filter Print Post Top
InvisiblePsilovibe
reader ofthreads

Registered: 11/14/04
Posts: 207
Loc: Treasure Coast
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: xburn]
    #4172955 - 05/13/05 10:14 PM (19 years, 9 days ago)

This is awesome workman. Great work, you obviously know your stuff :smile: I'm very impressed and interested in this. I look forward to seeing what they turn out like.

Extras: Filter Print Post Top
Offlinepeakpurity
Stranger

Registered: 12/21/20
Posts: 4
Last seen: 10 days, 22 hours
Re: Isolation of mycelium from dried mushrooms - Fruiting update [Re: Workman]
    #27530262 - 11/04/21 02:55 PM (2 years, 6 months ago)

amazing, this has me hopeful

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Does anyone know how to freeze dry mushrooms? *DELETED* Anonymous 7,064 12 01/23/06 07:09 AM
by Baby_Hitler
* mushroom fruit from sock like structure gray1 1,448 8 05/15/01 06:00 PM
by BrownPastures
* Dried fruit cloning? JDPhish 2,843 4 11/08/01 05:48 PM
by anima
* On the subject of drying Alkaloids 2,396 10 12/12/02 05:47 AM
by mycofile
* re-animate dried philosipher stones ???
( 1 2 all )
semilance 4,842 26 08/09/01 10:33 AM
by Anonymous
* Mushrooms as substrate? Anonymous 1,594 13 01/23/03 02:37 AM
by MAIA
* Mycelium & Genetic Exchange Gawain 3,241 13 10/23/02 09:42 PM
by Anonymous
* spores from dried caps kingkc 2,967 11 01/16/03 09:22 PM
by Zen Peddler

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
14,717 topic views. 0 members, 3 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.009 seconds on 14 queries.