|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Anno]
#3892827 - 03/09/05 01:20 PM (19 years, 2 months ago) |
|
|
lol. i'm sure it is still manufactured, or a related chemical is, if it proved useful (as clearly it does)
-------------------- buh
|
RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: shirley knott]
#3894872 - 03/09/05 08:20 PM (19 years, 2 months ago) |
|
|
From looking at that last pic, It looks like you rolled the dry tissue into the agar, thus several points of growth emerging. They appear to be growing together seamlessly, as opposed to forming sectors which is what one would expect if it were spores germinating. It looks to me like the dry tissue has begun to grow again. Is that your take as well?
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
Workman
1999 Spore War Veteran


Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: RogerRabbit]
#3897957 - 03/10/05 01:38 PM (19 years, 2 months ago) |
|
|
I did streak dried gill tissue over the surface using sterile forceps to spread out the spores and then leave the tissue on the agar at the end of the streak. Typically the tissue mass contaminates but spores left by the streaking usually germinate cleanly. I doubt there is anything but spores producing mycelium but there is no way of knowing. There are several mycelium types growing.
I did get a 10 pound bag of benlate as a lawn fungicide just before it was pulled from the market. It should last awhile using only 10mg amounts at a time.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
|
Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 8 days
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3903241 - 03/11/05 01:50 PM (19 years, 2 months ago) |
|
|
If you think there might be a contamination issue. Simply pour another layer of hot agar on top, completely covering the growth you have going. A whole new layer. The mycelium will crawl through to the top of the new layer of agar and will leave behind the contaminates. Then lightly scrape some of the new growth and transfer to a whole new dish. That should clean up your culture.
Edited by hotnutz (03/11/05 01:52 PM)
|
red914
Minion
Registered: 11/05/04
Posts: 134
Loc: Utah
Last seen: 18 years, 8 months
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Hotnuts]
#3926536 - 03/16/05 04:24 PM (19 years, 2 months ago) |
|
|
Hotnutz.. Just how hot would the agar be? sounds like a great plan.
|
MrMaddHatter
Dementia praecox


Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3928811 - 03/17/05 12:40 AM (19 years, 2 months ago) |
|
|
Quote:
Workman said: I did streak dried gill tissue over the surface using sterile forceps to spread out the spores and then leave the tissue on the agar at the end of the streak. Typically the tissue mass contaminates but spores left by the streaking usually germinate cleanly. I doubt there is anything but spores producing mycelium but there is no way of knowing. There are several mycelium types growing.
But a clean culture COULD be cultured out of this...........right???. (You would know!!)
I think its time for me to get back on the agar and get some experiments running.
There HAS to be a way to go from dried specimen to clean culture, no matter the contaminates. 
Pure cultures have been cultured from wild prints which have been exposed to any and everything outside, yet are still cultured out. I've done it myself.
|
Workman
1999 Spore War Veteran


Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: MrMaddHatter]
#3930088 - 03/17/05 11:06 AM (19 years, 2 months ago) |
|
|
Right, any obvious mycelium can be isolated away from contaminates. Sometimes it takes more than one attempt.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
|
red914
Minion
Registered: 11/05/04
Posts: 134
Loc: Utah
Last seen: 18 years, 8 months
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#3935090 - 03/18/05 10:25 AM (19 years, 2 months ago) |
|
|
I tried putting questionable spores in agar, and covering it with two sheets of boiled newspaper after cooling. The mycelium grew out to the outside layer. Peal the outer layer off to a fresh dish of agar. It appears clean. It would appear that contams do not like to be covered. I have also moved to the wide mouth jam jars to get away from the agar quickly drying out as the newspaper wicks off water fast. I tried spores alone in newspaper, but it works for oysters but not cubes, I guess they need more nutes.
|
Workman
1999 Spore War Veteran


Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#4123798 - 05/02/05 02:55 PM (19 years, 21 days ago) |
|
|
Results of attempted fruiting of above plate on grass seed cased with peat/calcium carbonate.
Day 11 after casing, incubated at 78F

-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
|
tahoe
Noob Slayer


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#4123890 - 05/02/05 03:21 PM (19 years, 21 days ago) |
|
|
Nice!!!
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
MrMaddHatter
Dementia praecox


Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#4128899 - 05/03/05 05:35 PM (19 years, 20 days ago) |
|
|
Very nice work!
|
Workman
1999 Spore War Veteran


Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#4133174 - 05/04/05 02:45 PM (19 years, 19 days ago) |
|
|
Its coming along.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
|
tahoe
Noob Slayer


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
#4133438 - 05/04/05 03:41 PM (19 years, 19 days ago) |
|
|
its nice to know that you are working to bring new strains that no one else has to the market
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
xburn
V card
Registered: 03/10/05
Posts: 707
Last seen: 11 years, 10 months
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: tahoe]
#4163283 - 05/11/05 06:48 PM (19 years, 12 days ago) |
|
|
why do you use grass seeed?
-------------------- Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.
|
Workman
1999 Spore War Veteran


Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 2 hours, 33 minutes
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: xburn]
#4163630 - 05/11/05 08:01 PM (19 years, 12 days ago) |
|
|
Ah, thats a good question. I use it because it seems to work well with a wide range of species. It is my first choice for specimens with unknown growth parameters.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
|
xburn
V card
Registered: 03/10/05
Posts: 707
Last seen: 11 years, 10 months
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: xburn]
#4165753 - 05/12/05 09:07 AM (19 years, 11 days ago) |
|
|
oh i see, workman i do wonder how would i get into doing the work that you do. I am facsinated nore by the mycology than the drugs and stuff.
-------------------- Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.
|
Psilovibe
reader ofthreads

Registered: 11/14/04
Posts: 207
Loc: Treasure Coast
|
Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: xburn]
#4172955 - 05/13/05 10:14 PM (19 years, 9 days ago) |
|
|
This is awesome workman. Great work, you obviously know your stuff I'm very impressed and interested in this. I look forward to seeing what they turn out like.
|
peakpurity
Stranger

Registered: 12/21/20
Posts: 4
Last seen: 10 days, 22 hours
|
Re: Isolation of mycelium from dried mushrooms - Fruiting update [Re: Workman]
#27530262 - 11/04/21 02:55 PM (2 years, 6 months ago) |
|
|
amazing, this has me hopeful
|
|