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OfflineWorkmanV
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Isolation of mycelium from dried mushrooms - Fruiting update
    #3762775 - 02/10/05 06:10 PM (11 years, 9 months ago)



Too bad there is so much condensation on these plates, but you can still get the idea.

Update 2/18/05

Well, that nice white mycelium isn't mushroom mycelium after all. A quick look under the scope revealed these odd looking structures.



They are the conidia of a species of Pestalotia, a genus of fungi that often causes disease in plants.

Pestalotiopsis link for more information.

It is a bummer and a bit embarrassing but such is the nature of science. I will redo the experiment with agar dosed with benlate fungicide to inhibit sporulating molds and allow the mushroom spores to germinate. Back to the drawing board.


Edited by Workman (05/02/05 05:33 PM)


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OfflineKevin
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3762907 - 02/10/05 06:36 PM (11 years, 9 months ago)

Nice man.  :thumbup:


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InvisibleJoshua
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3763421 - 02/10/05 08:06 PM (11 years, 9 months ago)

Since a gill fragment was taken, couldn't this be the germination of spores from a dried specimen versus the isolation of viable mycelium from a dried specimen?

Regardless though, this is good for people to know.

Joshua


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OfflineWorkmanV
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Joshua]
    #3763907 - 02/10/05 09:23 PM (11 years, 9 months ago)

Hmmm, yeah I can see how that is a bit confusing. I guess I should have said, "isolation of viable mycelium from spores attached to the gills of a dried mushroom".

Thanks for pointing that out.


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Offlinedmc_
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3764060 - 02/10/05 09:51 PM (11 years, 9 months ago)

Awesome picture workman.


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InvisibleYidakiMan
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: dmc_]
    #3764370 - 02/10/05 11:56 PM (11 years, 9 months ago)

Very cool. I am going to use this to get a culture of V. volvacea.


Did you use any antibiotic or hydrogen peroxide?


Edited by YidakiMan (02/10/05 11:56 PM)


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OfflineFluxburn
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: YidakiMan]
    #3764820 - 02/11/05 02:01 AM (11 years, 9 months ago)

wow workman I wish I knew similar to you so I could be your assistant, to bad for me.


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OfflineWorkmanV
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: YidakiMan]
    #3766614 - 02/11/05 02:56 PM (11 years, 9 months ago)

Oh yes, I used Gentamycin sulfate antibiotic in the agar. Sorry, I should have mentioned that before. I posted that image pretty late in the evening and it seems I left a lot of information out.


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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3768116 - 02/11/05 08:54 PM (11 years, 9 months ago)

I am always glad to see your work!

Joshua


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OfflineWorkmanV
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Re: Isolation of mycelium from dried mushrooms - Contamination update [Re: Workman]
    #3806580 - 02/20/05 02:15 PM (11 years, 9 months ago)

Oops, edited the top post to reveal a tactical error.


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Offlineshirley knott
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Re: Isolation of mycelium from dried mushrooms - Contamination update [Re: Workman]
    #3807219 - 02/20/05 05:21 PM (11 years, 9 months ago)



those are funky-looking thingies, workman, if unwanted. the antennae-thingies make them look like they're insects  :rolleyes:


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Offlinedebianlinux
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Joshua]
    #3827423 - 02/24/05 02:59 PM (11 years, 9 months ago)

Quote:

Joshua said:
I am always glad to see your work!

Joshua




agreed, i always ogle in amazement


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OfflineRogerRabbitM
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: debianlinux]
    #3837982 - 02/26/05 06:40 PM (11 years, 9 months ago)

Any updates?
When I first did this, I only used dry tissue from the stipe, to avoid getting spores. I wanted to make sure it was the mycelium recovering, not spores germinating.

Those are some cool looking cells, even if a contaminant. May I ask what type camera you're using?

The antenna looking things are known as cilia. They are used to create movement of the fluids around the cell.


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OfflineWorkmanV
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: RogerRabbit]
    #3842777 - 02/27/05 08:04 PM (11 years, 9 months ago)

2 days ago I put the gill tissue from several specimens on malt agar with benlate and gentamycin. Nothing to report yet. But I will update this post when something happens.

The camera is an old Sony Mavica FD87.


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OfflineMycelium5150
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3843362 - 02/27/05 09:48 PM (11 years, 9 months ago)

you still have that mavica! Isnt that the one that copies pics to floppies


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OfflineWorkmanV
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Mycelium5150]
    #3846830 - 02/28/05 03:12 PM (11 years, 9 months ago)

Yeah, the FD stands for Floppy disk. I have a Kodak DX4330 as well but the Kodak is just very handy to knock out web quality pics.


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OfflineWorkmanV
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3892742 - 03/09/05 02:58 PM (11 years, 8 months ago)

The benlate (10mg/liter) gentamycin sulfate (100mg/liter) attempt worked great. No signs of molds or bacteria at all. A vast difference compared to the earlier attempt with only gentamycin.



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InvisibleYidakiMan
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Workman]
    #3892792 - 03/09/05 03:11 PM (11 years, 8 months ago)

Are there any domestic stores that sell benlate? Feed stores?


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OfflineCyber
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: YidakiMan]
    #3892816 - 03/09/05 03:18 PM (11 years, 8 months ago)

I looked up benlate and found all kinds of stuff, Pulled from the market in 2001 because of birth defects! Kids born with out eyes, missing limbs, etc. Where did you get it?


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OfflineAnnoA
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Re: Isolation of viable mycelium from dried mushrooms - Pictorial [Re: Cyber]
    #3892821 - 03/09/05 03:19 PM (11 years, 8 months ago)

Before 2001 in the store I guess.


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