syringe question - Mushroom Cultivation

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heathadelic
member

Registered: 07/24/01
Posts: 159
Last seen: 23 years, 9 months

syringe question

#375843 - 08/21/01 07:59 PM (24 years, 3 months ago)

hey y'all. i'm a lucky gal who has access to an abundance of fresh syringes and a laminar flow hood. does anyone out there think i need to sterilize my syringes by autoclaving or boiling or something even if i can open them fresh inside of a flow hood?

Swing the groove 'round here, so I can reach it -a.d.

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"...time is not money, time is an opportunity to live before you die."

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dimitri211
Carpal Tunnel

Registered: 04/27/01
Posts: 2,248

Re: syringe question

[Re: heathadelic]
#375845 - 08/21/01 08:01 PM (24 years, 3 months ago)

As long as they are in the sterile packing when you open them no If they have been opened or used then yes autoclave or pc them

Dr Bluethumbs Old School Toadstools
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Shiznitz
addict

Registered: 04/19/01
Posts: 623
Loc: Canada
Last seen: 24 years, 22 days

Re: syringe question

[Re: heathadelic]
#375846 - 08/21/01 08:02 PM (24 years, 3 months ago)

If you bought them "guaranteed sterile", then there shouldn't be a problem. Sterilize them as a precaution if you want...

LEGALIZE...

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LEGALIZE...

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bakdorman
newbie

Registered: 07/27/01
Posts: 36
Last seen: 23 years, 10 months

Re: syringe question

[Re: Shiznitz]
#375937 - 08/21/01 09:57 PM (24 years, 3 months ago)

how long do you have to pc a syringe and needle to sterilize it??

The Federal Government Prohibits..........

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Shiznitz
addict

Registered: 04/19/01
Posts: 623
Loc: Canada
Last seen: 24 years, 22 days

Re: syringe question

[Re: bakdorman]
#376004 - 08/21/01 11:42 PM (24 years, 3 months ago)

You only PC them if they are empty, meaning they have no spores in them. If you get a syringe from a vendor that has spores in them, don't PC them, as this will kill the spores... (Common Sense)

PC syringes for 45 min at 15 psi

LEGALIZE...

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Anno
Experimenter

Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 1 month, 29 days

Re: syringe question

[Re: Shiznitz]
#376015 - 08/22/01 12:08 AM (24 years, 3 months ago)

> PC syringes for 45 min at 15 psi
Syringes heat up much faster then the inside of a jar.
20 minutes in a pc is sufficient, but you can allways do more....

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Zen Peddler

Registered: 06/18/01
Posts: 6,379
Loc: orbit

Re: syringe question

[Re: bakdorman]
#376152 - 08/22/01 03:51 AM (24 years, 3 months ago)

New fangled backdoorman - make sure you have some water in those when you PC them, say half full...and reassemble them while still in the pressure cooker and hot steam...

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bakdorman
newbie

Registered: 07/27/01
Posts: 36
Last seen: 23 years, 10 months

Re: syringe question

[Re: Zen Peddler]
#376353 - 08/22/01 11:05 AM (24 years, 3 months ago)

thanks

The Federal Government Prohibits..........

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