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Offlinewatertrade2003
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Registered: 07/11/04
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Loc: Canberra Australia
Last seen: 12 years, 14 days
dried mushrooms - spores active??
    #3752262 - 02/08/05 08:24 PM (12 years, 7 months ago)

Hi,
i reciently bought some sliced reishi to make tea and found that a piece of mushroom that was not sliced has a thin layer of what I think could be spores sitting on the surface - has anyone had success with dried mushrooms?? would the processing and storage (possibly for years) render the spores dead/inactive??

it would be great if I could get them started on some agar. !

thanks Jim


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Invisibletahoe
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Registered: 11/27/03
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Re: dried mushrooms - spores active?? [Re: watertrade2003]
    #3752457 - 02/08/05 09:09 PM (12 years, 7 months ago)

they should be viable. I took a gill off of a dried shittake and started it on agar


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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OfflineYummymush
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Registered: 11/17/04
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Re: dried mushrooms - spores active?? [Re: tahoe]
    #3757075 - 02/09/05 06:36 PM (12 years, 7 months ago)

Check recent Booknik's thread. He successfully grows some maitake from spores collected from dried mushrooms! I'm trying spores from maitake right now as well.

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3573750/an/0/page/2


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Offlinewatertrade2003
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Registered: 07/11/04
Posts: 17
Loc: Canberra Australia
Last seen: 12 years, 14 days
Re: dried mushrooms - spores active?? [Re: watertrade2003]
    #3765538 - 02/11/05 07:03 AM (12 years, 7 months ago)

The story so far...
I transfered what i Thought to be spores and small sections of the tube parts of the reishi on to 10 plain malt agar plates (no h2o2 - thinking that spores would be killed by the h2o2-- ??? right?)

so far I have ( after 24 hours)@ about 27C

2 plates lost to the usual white ring bacteria that loves my house.

2 plates that have very thin thread like growth from the excised mushroom ( I think its cobweb mold- ??? - it doesn't fit the reishi mycelium charastics GGMM p357 ) - very thin aerial growth over the plate

1 plate with a small but thick ring of growth which I'm hopeing is mycelium.

all transfers were done in a glove box using alcohol to clean all tools.

I'm just wondering...  can all types of mushrooms synthesise the enzymes to break down hydrogen peroxide?? -  If I try and transfer the thick healthy looking plate onto h2o2 malt agar,  would this work?  help me sleep at night? :wink:

cheers
Jim  :smile:


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InvisibleATWAR
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Registered: 01/26/03
Posts: 1,640
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Re: dried mushrooms - spores active?? [Re: watertrade2003]
    #3766049 - 02/11/05 11:57 AM (12 years, 7 months ago)

Quote:

watertrade2003 said:
can all types of mushrooms synthesise the enzymes to break down hydrogen peroxide??




I don't think so. While most species will grow on peroxidated agar, some species are severely hindered by it, and some refuse to grow (concentration has a lot to do with it as well).


Quote:

If I try and transfer the thick healthy looking plate onto h2o2 malt agar, would this work? help me sleep at night?




Most likely. I don't know about helping you sleep though...


--------------------
To give is to live...



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Offlinewatertrade2003
loving it...
Registered: 07/11/04
Posts: 17
Loc: Canberra Australia
Last seen: 12 years, 14 days
Re: dried mushrooms - spores active?? [Re: ATWAR]
    #3767449 - 02/11/05 06:10 PM (12 years, 7 months ago)

...  after a great nights sleep I woke to find all the plates contaminated. I will have to get some reishi the old fashioned way and buy a wedge. 
thanks for the help anyway!

cheers
Jim
:laugh:


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