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OfflineWorkmanV
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: tregar]
    #3857371 - 03/02/05 01:17 PM (19 years, 2 months ago)

I used special presealable bags with injection port. They are more expensive but I get them free for my efforts. They have a special filter that vents faster to prevent bursting.

I didn't use a recipe. It is just horse manure, mostly dung with some grass or straw mixed in (not added but present in the manure when gathered). It was pretty wet when I got it so I squeezed out as much water as I could and then broke it up into a fluffy texture. I then loaded the bags, impulsed sealed them closed and sterilized at 15psi for 2 hours.

Earlier I had made a dilute malt water broth (1tsp:2cups) with a bit of the manure water from the squeezing. Sterilized this for 20 minutes at 15psi and inoculated with a pan cyan agar wedge when cool. This liquid inoculate was colonized in just a few days and was ready to draw into syringes and inject into the bags. I injected a full 10cc into the manure bag and growth was noticable in 24 hours at 85F. 10 days later (with one shaking) the bag was ready to be cased and 7 days later the first mushrooms were harvested.


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Offlinetregar
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
    #3864176 - 03/03/05 06:20 PM (19 years, 2 months ago)

thanks for explaining, great job.

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Offlinepsiloz
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Registered: 06/03/03
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Loc: Australia
Last seen: 19 years, 17 days
Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: tregar]
    #4049972 - 04/13/05 11:21 AM (19 years, 1 month ago)

Those robust Pans look just like the big var of pans in the fields of nthn NSW
identical in size and stature

could be the same strain/species

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InvisibleGOATOAD
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
    #18329743 - 05/27/13 07:01 PM (10 years, 11 months ago)

:thumbup:  NICE looking pan grow!

I've been a lil hesitant to jump into a pan grow bc I heard they can be finicky for some. Yet I've heard others say pans exhibit rigorous growth even though the mycelium tends to be whiskier. Seeing grows and information like this gives me encouragement to give it a try.

Quick question: How do pans fair when working with LC's (H2O & honey MIX)? Would you foresee any problem directly injecting a spore syringe directly into the LC?

Thanks again! There's some good information here!

:rockon:

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OfflineWorkmanV
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: GOATOAD]
    #18332689 - 05/28/13 10:21 AM (10 years, 11 months ago)

Holy crap this thread is old. 

Only a very clean spore syringe will work in an LC, so it isn't recommended.  There can be low levels of bacteria in a spore syringe that ruins an LC, but occasionally it works.


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OfflineHazeyRoms
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Registered: 07/19/08
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Loc: Wisconsin
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
    #18342453 - 05/30/13 09:07 AM (10 years, 11 months ago)

Good job Workman, always a fan. Ain't been to the forum in a minute.


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OfflineTruth.Proof.Power
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Registered: 04/02/13
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
    #18394235 - 06/09/13 08:34 PM (10 years, 11 months ago)

do you fruit it with no fruiting chamber?

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