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OfflineChrisHippyLoser
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Registered: 12/22/04
Posts: 120
Loc: Big Tex
Last seen: 11 years, 4 months
idea for easy syringe making method
    #3751059 - 02/08/05 03:14 PM (12 years, 18 days ago)

ok...here's an idea i just had for making spore syringes...i don't think its extremely original, but i can't remember where i saw a similar tek...

supplies:
1- plastic solo cup
2- quality water (maybe tap, but i'd buy bottled)
3- tin foil
4- rubbing alcohol
5- pressure cooker
6- ripe and ready caps
7- sterile syringe

boil some of the water and suck up 10 to 12cc's into the syringe (now the inside of the syringe should be sterile if it wasn't already). take the plastic disposable cup and cover the top with tin foil. stick the syringe needle into the cup near the top and squirt the water into the cup. cover the needle hole with electrical tape. set the syringe aside and get out the pressure cooker. put a towel on the bottom of the pc and fill with some water. place the cup with the tin foil top in the pc. pc it for like 30 minutes. then let cool. then take out the cup (hopefully i hasn't melted, i'm not so sure about that part yet).

now go find your specimen cap and get some more tinfoil. clean up the tinfoil with rubbing alcohol. pick the cap with the tinfoil (like you would pick up dog shit with a paper towel). fashion the tin foil so that it cradles the cap, but allows plenty of open space for the spores to drop, just kinda curl up the tinfoil around the edges of the cap.

get your pc'd cup ready and very quickly replace the tinfoil top with the new tinfoil peice that has the cap in it. then set it aside for about 24 hours.

i'm thinking that the spores should drop into the water then you can just kinda swirl it up some, poke your needle in and suck of the water.

no need for a spore print, spores drop directly into the water. plus i'm thinking this cuts down on oportunities for contams.

good idea, bad idea? i have to be overlooking something, whether its the fact that the plastic cup will melt or maybe spores dropping directly into water would be bad...i dunno...

lookin for opinions here. my friend is gonna want to produce some spore syringes here in the next couple of days and i wanna get him good info on how to do it.

thanks all! he never could have gotten to this point without this board and its many generous and knowledgable members.


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InvisiblePrisoner#1M
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Re: idea for easy syringe making method [Re: ChrisHippyLoser]
    #3751063 - 02/08/05 03:16 PM (12 years, 18 days ago)

how do you sterilize the cup


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Invisiblemetasin
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Re: idea for easy syringe making method [Re: ChrisHippyLoser]
    #3751065 - 02/08/05 03:17 PM (12 years, 18 days ago)

The solo cup has a 6 on the bottom of it. AFAIK the only plastic that can withstand a PC has a 5. I'm still working the keg from the superbowl thing and I got one of the cups in front of me now.


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Invisiblemetasin
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Re: idea for easy syringe making method [Re: Prisoner#1]
    #3751067 - 02/08/05 03:17 PM (12 years, 18 days ago)

Said put it in the PC


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InvisiblePrisoner#1M
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Re: idea for easy syringe making method [Re: metasin]
    #3751079 - 02/08/05 03:20 PM (12 years, 18 days ago)

Quote:

recondite said:
AFAIK the only plastic that can withstand a PC has a 5.





some polypropylenes and all polymethylpentenes will withstand PC temps


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OfflineChrisHippyLoser
Dope Smoker

Registered: 12/22/04
Posts: 120
Loc: Big Tex
Last seen: 11 years, 4 months
Re: idea for easy syringe making method [Re: metasin]
    #3751080 - 02/08/05 03:20 PM (12 years, 18 days ago)

right on then...i guess we'll just have to go with the more traditional methods...

good job on the keg brotha! good men always see things through...you're not done drinkin till that sucker is floating and spittin foam...hell yeah!

edit - so from the last post it seems maybe the cup would hold out...i think we'll have to experiment some.... so the spores going directly into the water is ok? i figured it would be, but wasn't positive. and the cup is sterilized in the pc with the water that was shot in from the syringe. i know that the water is now double sterilized, so the begining part with boiling the water and sucking it into the syringe and all could be skipped if you've got a sterile packaged syringe to suck spore solution into and an old syringe you can use just to measure water with, or hell whatever you want to do to measure the initial 12cc's that go into the cup is fine.

and also, use yer head...flame sterilize needles and wear a mask and everything... i guess this idea is more for people who don't have a flowhood or glovebox and are paranoid about sterility...and for someone who has never made a spore print or syringe before and isn't quite sure how easily a print will pick up unwanted contams


Edited by ChrisHippyLoser (02/08/05 03:30 PM)


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InvisiblePrisoner#1M
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Re: idea for easy syringe making method [Re: ChrisHippyLoser]
    #3751135 - 02/08/05 03:35 PM (12 years, 18 days ago)

Quote:

ChrisHippyLoser said:
edit - so from the last post it seems maybe the cup would hold out...




solo cups are made from polystyrene, they have the lowest melting point of all plastics, the 2 commonly available plastics that will hold up the best are
marked on the bottom with a #2 and a #5, the #2 plastics, HDPE is milk cartons
the recycle them to make Tyvek, tyvek certainly hold together in a PC


here's magash's list a little clearer




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Invisiblemetasin
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Re: idea for easy syringe making method [Re: Prisoner#1]
    #3751140 - 02/08/05 03:37 PM (12 years, 18 days ago)

nice chart, very helpful!!


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InvisiblePrisoner#1M
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Re: idea for easy syringe making method [Re: metasin]
    #3751187 - 02/08/05 03:46 PM (12 years, 18 days ago)

blame that one on Magash, I just provided one thats a little easier to read,
HDPE has a melting point between 110c and 160c (230F-320F) it's dependant on the manufacturing method, polypropylene (#5) is about 160c (327F).


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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: idea for easy syringe making method [Re: Prisoner#1]
    #3751311 - 02/08/05 04:28 PM (12 years, 18 days ago)

quick question, if he's gonna be pc'ing, then why not just pc the syringes? it'd save a couple steps... just a thought...


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Invisibletahoe
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Re: idea for easy syringe making method [Re: NeedMoreSleep]
    #3751716 - 02/08/05 06:28 PM (12 years, 17 days ago)

how about this
Half pint jar, large or small mouth. Stamets prefers small
fill 1/2-3/4 with water. tyvek it and sterlize, 15-20 mins at 15 psi.
get sterlile syringe and suck up cool water from pint jar.
take print make sure its folded in half and use needle of the same syringe to scrape the print loose so that it is in the crease of the paper.
scrape print into the needle sleeve so that its abouthalf full
put needle back into sleeve and squirt a drop of water into it and suck it up.
do this a few times tell most of the print is in the syringe.
squirt syringe into the pint jar and seal with tape.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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