Home | Community | Message Board


SoulSpeciosa Kratom
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 11 years, 8 months
How to spot liquid culture contamination?
    #3742782 - 02/06/05 11:30 PM (11 years, 9 months ago)

I have 2 liquid cultures that have plenty of growth in them but I am recently suspecting them of contamination. There are 2 spots in which the growth seems especially white. It is especially tough for me to determine anything because they are honey cultures. On one it is a thick, bright white growth floating on the surface like some nasty spit. Neither of them have a foul odor coming from them. Is there any way to determine whether these are contaminated or not?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: How to spot liquid culture contamination? [Re: spliffmasta]
    #3742844 - 02/06/05 11:41 PM (11 years, 9 months ago)

Sometimes, a bit of myc (looks bright white) will start to grow on the surface & spread. Sounds like that's what you have.

Anything blue, green, funky cinimon gray, slimy like vegatable oil, curtled milk or tiny black dots, it has a bad case of the clap.


Healthy dogs


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,418
Loc: Nowhere
Re: How to spot liquid culture contamination? [Re: agar]
    #3742933 - 02/06/05 11:57 PM (11 years, 9 months ago)

Do those have injection plugs on the bottom, or am I seeing things?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 11 years, 8 months
Re: How to spot liquid culture contamination? [Re: MrMaddHatter]
    #3743877 - 02/07/05 03:10 AM (11 years, 9 months ago)

Do liquid culture contaminations typically smell?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineZeroArmy27
I didn't go to work for a month.
 Arcade Champion: Asteroids 2000

Registered: 09/01/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
Re: How to spot liquid culture contamination? [Re: spliffmasta]
    #3743884 - 02/07/05 03:12 AM (11 years, 9 months ago)

mrmadhatter, i believe you are seeing things.


--------------------
"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: How to spot liquid culture contamination? [Re: spliffmasta]
    #3744427 - 02/07/05 09:21 AM (11 years, 9 months ago)



Here is an example of myc creating an island on the surface of a LC.

No contam, at all. (this was one of my experment recipe jars)


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescooterpop
Foolish Mortal

Registered: 01/08/05
Posts: 56
Loc: 3rd Stone from the Sun
Last seen: 10 years, 8 months
Re: How to spot liquid culture contamination? [Re: agar]
    #3746175 - 02/07/05 05:42 PM (11 years, 9 months ago)

agar - what's the medium in those quarts in your 1st pic (healthy dogs)? if karo, what's the ratio?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShortyMcAffy
n00blar

Registered: 01/02/05
Posts: 71
Loc: Pennsylvania USA
Last seen: 10 years, 3 months
Re: How to spot liquid culture contamination? [Re: scooterpop]
    #3746238 - 02/07/05 05:54 PM (11 years, 9 months ago)

Could someone direct me to a guide for making/using liquid culture?


--------------------
Do you like GLASS PIPES?

If you answered yes to the question above then check out this website.
http://community.webshots.com/user/shortymcaffy


If you want to know how to get some PM me.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: How to spot liquid culture contamination? [Re: scooterpop]
    #3746255 - 02/07/05 05:57 PM (11 years, 9 months ago)

Healthy dogs is 2 each heaping teaspoons light dry malt / dry dextrose (think home brew & wine making supply store) to quart distilled water.

Throw it all in stove top pot, stir well, then boil, cool some, strain through coffee filter to remove any carmalized tid bits, jar up & PC 20 / 25 minutes @ 15 psi.



Lid that allows filtered air exchange HELPS GREATLY.

Simple to build with drill / bit out of rubber tubing, some high temp RTV silicone & polyfil.

Another advantage to that type lid is asperation of liquid culture be removing polyfil, inserting syringe into tubing, tilt jar & sucking LC out. Minimal exposure to unfiltered air....... is a good thing with LC.

Also, if you have stir plate..... it is great advantage.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: How to spot liquid culture contamination? [Re: ShortyMcAffy]
    #3746313 - 02/07/05 06:08 PM (11 years, 9 months ago)

Quote:

ShortyMcAffy said:
Could someone direct me to a guide for making/using liquid culture?




Here is some


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVertigo6911
Entheobotanist
Male User Gallery

Folding@home Statistics
Registered: 12/04/04
Posts: 1,834
Loc: Netherlands
Last seen: 10 years, 28 days
Re: How to spot liquid culture contamination? [Re: agar]
    #3793661 - 02/17/05 04:09 PM (11 years, 9 months ago)



its white... but it looks like ... spunk...
is it myc?


--------------------
-Know ye not that ye are gods?-
My homepage


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedjsage420
aka DJQBNSIS
 User Gallery

Registered: 02/15/05
Posts: 869
Loc: Panhandle
Last seen: 10 years, 8 months
Re: How to spot liquid culture contamination? [Re: Vertigo6911]
    #3793672 - 02/17/05 04:11 PM (11 years, 9 months ago)

looks like it but a little early to tell


--------------------
If you put your head to the grass, you can hear it growing


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVertigo6911
Entheobotanist
Male User Gallery

Folding@home Statistics
Registered: 12/04/04
Posts: 1,834
Loc: Netherlands
Last seen: 10 years, 28 days
Re: How to spot liquid culture contamination? [Re: djsage420]
    #3793750 - 02/17/05 04:37 PM (11 years, 9 months ago)

kewl thxz


--------------------
-Know ye not that ye are gods?-
My homepage


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Liquid Culture Contamination Attempt and Failure.. in a good way? Quiescence 3,459 14 06/24/05 02:23 PM
by scatmanrav
* Liquid culture from Liquid culture. matits 5,334 19 07/13/13 01:34 AM
by MattyBear
* Liquid Culture Contam? (White fuzzy balls) kwikness 3,242 1 03/28/06 09:50 PM
by Rahz
* liquid culture contaminated?/w pics added UPDATE
( 1 2 all )
califarmer619 3,410 20 11/17/08 07:11 PM
by HybridprX
* is my liquid culture contaminated?
( 1 2 all )
billycorgan55 3,097 23 02/09/09 06:35 PM
by Boris
* liquid culture contamination? merryprankster91 636 0 02/02/07 03:46 PM
by merryprankster91
* Buckaroo Bulk Nugget Tek - grain to bulk in one sealed container
( 1 2 all )
buckaroobanzai 13,604 23 09/12/06 06:27 AM
by fingers
* Agar vs. Liquid Culture Question KauaiOrca 2,370 16 08/15/08 12:29 AM
by just me

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
6,113 topic views. 9 members, 45 guests and 33 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.004 seconds on 14 queries.