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Offlineazurescens
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Registered: 02/17/04
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Growth after freezing!!!!
    #3741933 - 02/06/05 08:24 PM (13 years, 3 months ago)

I posted a few weeks ago asking if myc would survive freezing temps. After receiveing mixed responses at best, I decided to do my own experiment. 4 agar plate with good growth were placed into a freezer for 1,2,3,4 days respectively. Upon taking out and placing back into environment of temps 0f 82-86....ALL 4 PLATES resumed growth and are still growing healthily. Hope this answers some questions concerning this matter. Strains were

plate 1------Equador
2------------KSS
3------------KSS
4------------KSS


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OfflinekronnyQ
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Re: Growth after freezing!!!! [Re: azurescens]
    #3741994 - 02/06/05 08:35 PM (13 years, 3 months ago)

Interesting....any plans to test myc frozen for long periods of time e.g. weeks, months?


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OfflineMushroomFriend
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Re: Growth after freezing!!!! [Re: azurescens]
    #3741998 - 02/06/05 08:36 PM (13 years, 3 months ago)

Thanks for testing! :laugh: How cold did they get?


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Offlineazurescens
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Re: Growth after freezing!!!! [Re: MushroomFriend]
    #3742087 - 02/06/05 09:00 PM (13 years, 3 months ago)

Just as cold as my freezer which is below 32 whereas the water freezes but I idnt/dont have a thermometer. And yes, I do have plans for future exp regarding longer periods of exposed time. Although I must admit, it makes me sad to think of wasting good cultures of such sweet myc.


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Offlineazurescens
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Re: Growth after freezing!!!! [Re: azurescens]
    #3742941 - 02/06/05 11:58 PM (13 years, 3 months ago)

:blush: :blush: :blush:


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OfflineAnnoA
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Re: Growth after freezing!!!! [Re: azurescens]
    #3861492 - 03/03/05 10:05 AM (13 years, 2 months ago)

Interesting....


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Invisibletripndicular
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Re: Growth after freezing!!!! [Re: azurescens]
    #3861528 - 03/03/05 10:20 AM (13 years, 2 months ago)

I remeber that post , ANNO was also doing some basic research on it. Oh no that was with syringes.
Ya know this draws another question .
I wonder if during the growth of a culture...if one developed some sort of contam growth on the petri ...if freezing it for a few days might kill off the invader leaving behind the myc. Many if not most bacterial invaders can be killed off by freezing them. HUM..... wonder what freezing a casing that has seen some nasties might work hmmmmmmmmm much interesting angles to approach possible ways to kill off contams.
Keep us posted to your findings plz!
GL


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OfflinekronnyQ
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Re: Growth after freezing!!!! [Re: tripndicular]
    #3861581 - 03/03/05 10:41 AM (13 years, 2 months ago)

You know, thats a good idea.

I have some casings that I know are infected with Bacilus and cobweb....putting one in the freezer right now.


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