Not to bump an old thread, but I thought there might be something worth noting here. This may sound like a headache, so if you're not in the mood for research, my advice would be to NOT read this next post until you ARE in the right mood. Reading this post may take multiple hours depending on the level of familiarity you have with these topics.
First, allow me to very briefly go over some of the chemicals detected in psilocybin mushrooms, along with some potentially mind-altering notes.
You've all probably read tons of literature on the following compounds, which I will capitalize below, rather than leaving them un-capitalized:
1. Psilocybin
2. Psilocin
3. Baeocystin (Baeocystine)* = misnomer
4. Nor-Baeocystin* = misnomer
5. Aeruginascin
6. Nor-Psilocin
* Note: According to author Paul Stamets, the chemical currently known as baeocystin (baeocystine) was incorrectly named. A misnomer occurred as a result of a mis-identified collection of what was actually Psilocybe cyanescens (and not Psilocybe baeocystis, as was originally published). He proposed cyanescin as the new chemical name, and hinted it could be studied for human therapeutic potential. This would also cause nor-baeocystin to be called nor-cyanescin. See this and fast forward to about 58 minutes through.
See also the important paper titled, Injury-Triggered Blueing Reactions of Psilocybe Magic Mushrooms. Substrates designed to increase psilocybin may also play a significant role in potency and chemical profiles. The third harvested flush of some strains may also play a significant role. As mentioned by others, genetics appear to be the ultimate pathway for altering potency. This requires effort, stamina, and lab gear.
Psilocybin mushrooms also all appear to develop significant quantities of various MAOIs (MonoAmine Oxidase Inhibitors). See this.
See the book called Psychedelics Reimagined by Thomas Lyttle. It includes a section/chapter titled, Psychedelic Monographs and Essays, Volume Six (1993). In it is a page or more about adding dextrose to the substrate (rye in this case) to achieve higher psilocybin content.
However, one mycologist wrote on this site many years ago that this was not the right way to go about it. This particular mycologist said,
Quote:
"I don't see why dextrose would increase psilocybin content. If you really want to increase psilocybin content, add Diammonium Phosphate and non-fat powdered milk to the substrate. Diammonium Phosphate has the phosphorus needed to make psilocybin, and non-fat powdered milk has the tryptamines."
However, it seems adding tryptamine may also increase psilocybin and psilocin levels.
Jochen Gartz's recipe, as mentioned in previous comments, reciprocally calls for 1/2 pint PF Tek jars: 1/2 - 2/3 cup of vermiculite + 1/8 cup of brown rice powder and (45 milliliters of water with .1 grams of Tryptamine HCL added). Any more than .1 g will cause poorly formed, dwarf-like specimens. However, as experiments in this thread have mentioned, this recipe is now strongly under scrutiny.
As a final stimulating note here, all of the notes provided here require lab gear to confirm these notes or disprove these notes. That lab gear will almost certainly require Liquid Chromatography/Mass Spectrometry. This requires more effort than all members of The Shroomery appear to be willing to put in on this interesting topic. I see a lot of under study, and a massive amount of laziness regarding this topic. If there are literally about 200 species of fungi confirmed for the presence of one or more chemicals relevant to this post, that means someone - or a team - needs to test each species of each relevant genus. Perhaps the person on this resource could lead such an effort.
More For Those Willing To Push The Envelope Of Science:
https://www.agilent.com/cs/library/support/documents/a05296.pdf
https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/617755bc913a74cab06a8a2d/original/rapid-quantification-of-psilocybin-with-reversed-phase-hplc-and-single-wavelength-detection.pdf
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