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OfflineFluxburn
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Registered: 10/23/04
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spore prints can be contaminated?
    #3740480 - 02/06/05 01:32 PM (13 years, 10 months ago)

Hey I got some spore prints but was rather stupid and made a syringe using the oven tech, which didn't work at all and the syringe has other bacteria spores in it.

My question is now that I opened the spore print, I must assume that it is now fully contaminated? You have to open spore prints in a controlled environment, like a glove box but how come you can store them just in a bag?

Some light on this issue would be nice, thanks.


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OfflineKaptKid
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Re: spore prints can be contaminated? [Re: Fluxburn]
    #3740490 - 02/06/05 01:40 PM (13 years, 10 months ago)

Your right. Need Flow hood or glove box. Improves chances greatly.

GL


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OfflineFluxburn
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Re: spore prints can be contaminated? [Re: KaptKid]
    #3740511 - 02/06/05 01:49 PM (13 years, 10 months ago)

sucks a nut hardcore, I gotta laugh as my agar plates are not contaminated (at least no growth other than white) but my damn syringes get contaminated. I guess the game for not getting the hepa airhood is over. I will have to use a glove box in the mean time to hold me off, since I can't afford it!

someone should make a damn hepa airhood kit, everyone here would buy one if it was $200-300.


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Offlinedelphis
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Re: spore prints can be contaminated? [Re: Fluxburn]
    #3740626 - 02/06/05 02:46 PM (13 years, 10 months ago)

i got spores from my first contaminated growing experience, i'll try to inoculate them and then'll see what happens..


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Offlineprototypical_man
ass model

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Re: spore prints can be contaminated? [Re: delphis]
    #3741219 - 02/06/05 05:21 PM (13 years, 10 months ago)

try doing a karo tek with your contamed spores. add a good dose of h2o2 once you see some myc.


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OfflineAmygdala
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Re: spore prints can be contaminated? [Re: prototypical_man]
    #3741242 - 02/06/05 05:25 PM (13 years, 10 months ago)

Quote:

prototypical_man said:
try doing a karo tek with your contamed spores.




May I ask why anyone would want to do such a thing?

On a quick sidenote, how is it that you have a trade rating mere hours after your account was created? tsk tsk...


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"Only two things are infinite, the universe and human stupidity, and I'm not sure about the former." --Albert Einstein


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InvisibleLouiseLouise
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Re: spore prints can be contaminated? [Re: Amygdala]
    #3741336 - 02/06/05 05:51 PM (13 years, 10 months ago)

Good question.


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"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


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InvisiblePrisoner#1
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Re: spore prints can be contaminated? [Re: Fluxburn]
    #3741350 - 02/06/05 05:56 PM (13 years, 10 months ago)

Quote:

Fluxburn said:
someone should make a damn hepa airhood kit, everyone here would buy one if it was $200-300.





one sorta like this



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Offlineorichalc
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Re: spore prints can be contaminated? [Re: Prisoner#1]
    #3741369 - 02/06/05 06:00 PM (13 years, 10 months ago)

nice


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OfflineAmygdala
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Re: spore prints can be contaminated? [Re: orichalc]
    #3745592 - 02/07/05 03:32 PM (13 years, 10 months ago)

I second that...

NICE  :grin:

prototypicalman... no comment?  :smirk:


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"Only two things are infinite, the universe and human stupidity, and I'm not sure about the former." --Albert Einstein


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OfflineFluxburn
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Re: spore prints can be contaminated? [Re: Amygdala]
    #3777055 - 02/14/05 03:00 AM (13 years, 9 months ago)

how the hell did u make that?


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OfflineVertigo6911
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Re: spore prints can be contaminated? [Re: Fluxburn]
    #3777129 - 02/14/05 03:31 AM (13 years, 9 months ago)

scuse me but doesent liquid culture exist specificly so u can use
dirty sporeprints?

just make a bunch of plates, some will be contammed some will be clean... no reason to trow out the print yo.


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: spore prints can be contaminated? [Re: Vertigo6911]
    #3777136 - 02/14/05 03:35 AM (13 years, 9 months ago)

Prisoner did u make or buy that?


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OfflineCyber
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Re: spore prints can be contaminated? [Re: LengthyFellow]
    #3777525 - 02/14/05 08:16 AM (13 years, 9 months ago)

To clean up a contaminated print you will have to use agar and culture it out. Karo does nothing, the contamination will grow in karo just as good as the spores.

Why spend $200 on a flow hood when you can bild a glove box for $20





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OfflineVertigo6911
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Re: spore prints can be contaminated? [Re: Cyber]
    #3777531 - 02/14/05 08:20 AM (13 years, 9 months ago)

nice glovebox... the thing i wonder about those is how to make sure u dont introduce contams when u place ure equiptment in the chamber...


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OfflineCyber
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Re: spore prints can be contaminated? [Re: Vertigo6911]
    #3777542 - 02/14/05 08:29 AM (13 years, 9 months ago)

That would depend on how you use it.

I have had my Glove Box hooked up to a hepa filter, In those instances it is a forced air box.

If you are using it with out forced air you will load it, let it set for a while, then use your sterilizing agent (alcohol or lysol) inside the box. Then let it set for a while.


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OfflineVertigo6911
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Re: spore prints can be contaminated? [Re: Cyber]
    #3777559 - 02/14/05 08:45 AM (13 years, 9 months ago)

kewl thxz


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