Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, HEPA Filter, Laminar Flow Hood

Jump to first unread post. Pages: 1
OfflineFluxburn
.
 User Gallery

Registered: 10/23/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 6 years, 4 months
spore prints can be contaminated?
    #3740480 - 02/06/05 01:32 PM (12 years, 1 month ago)

Hey I got some spore prints but was rather stupid and made a syringe using the oven tech, which didn't work at all and the syringe has other bacteria spores in it.

My question is now that I opened the spore print, I must assume that it is now fully contaminated? You have to open spore prints in a controlled environment, like a glove box but how come you can store them just in a bag?

Some light on this issue would be nice, thanks.


--------------------
ABSTRACT ART (Mine) http://nathanbelomy.com


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKaptKid
Spaced Pirate
Male User Gallery

Registered: 12/11/03
Posts: 6,248
Loc: Bright Side of the Sun
Last seen: 9 days, 8 hours
Re: spore prints can be contaminated? [Re: Fluxburn]
    #3740490 - 02/06/05 01:40 PM (12 years, 1 month ago)

Your right. Need Flow hood or glove box. Improves chances greatly.

GL


--------------------
Child of the 60's, Tripping ever since.


:sun:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFluxburn
.
 User Gallery

Registered: 10/23/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 6 years, 4 months
Re: spore prints can be contaminated? [Re: KaptKid]
    #3740511 - 02/06/05 01:49 PM (12 years, 1 month ago)

sucks a nut hardcore, I gotta laugh as my agar plates are not contaminated (at least no growth other than white) but my damn syringes get contaminated. I guess the game for not getting the hepa airhood is over. I will have to use a glove box in the mean time to hold me off, since I can't afford it!

someone should make a damn hepa airhood kit, everyone here would buy one if it was $200-300.


--------------------
ABSTRACT ART (Mine) http://nathanbelomy.com


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedelphis
wanderer
Registered: 11/04/04
Posts: 14
Last seen: 11 years, 9 months
Re: spore prints can be contaminated? [Re: Fluxburn]
    #3740626 - 02/06/05 02:46 PM (12 years, 1 month ago)

i got spores from my first contaminated growing experience, i'll try to inoculate them and then'll see what happens..


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineprototypical_man
ass model

Registered: 02/02/05
Posts: 396
Loc: Doral Florida
Last seen: 1 year, 1 month
Re: spore prints can be contaminated? [Re: delphis]
    #3741219 - 02/06/05 05:21 PM (12 years, 1 month ago)

try doing a karo tek with your contamed spores. add a good dose of h2o2 once you see some myc.


--------------------
I have 29 Strains as of current.. I LOVE TO TRADE. See my bio for the strains I currently have.

I seek: Strains I don't have, exotic species, Edibles, Vacutainers, A accurate scale, chicken manure, worm castings, worms for making my own worm castings, plastic lids for mason jars, light malt extract, Kelp Meal, A Ph Tester, A microscope.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAmygdala
Learner

Registered: 06/30/04
Posts: 158
Last seen: 1 year, 7 months
Re: spore prints can be contaminated? [Re: prototypical_man]
    #3741242 - 02/06/05 05:25 PM (12 years, 1 month ago)

Quote:

prototypical_man said:
try doing a karo tek with your contamed spores.




May I ask why anyone would want to do such a thing?

On a quick sidenote, how is it that you have a trade rating mere hours after your account was created? tsk tsk...


--------------------
"Only two things are infinite, the universe and human stupidity, and I'm not sure about the former." --Albert Einstein


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleLouiseLouise
starstruck
Male User Gallery

Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
Re: spore prints can be contaminated? [Re: Amygdala]
    #3741336 - 02/06/05 05:51 PM (12 years, 1 month ago)

Good question.


--------------------
"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: spore prints can be contaminated? [Re: Fluxburn]
    #3741350 - 02/06/05 05:56 PM (12 years, 1 month ago)

Quote:

Fluxburn said:
someone should make a damn hepa airhood kit, everyone here would buy one if it was $200-300.





one sorta like this



Post Extras: Print Post  Remind Me! Notify Moderator
Offlineorichalc
Stranger

Registered: 01/30/05
Posts: 71
Last seen: 11 years, 1 month
Re: spore prints can be contaminated? [Re: Prisoner#1]
    #3741369 - 02/06/05 06:00 PM (12 years, 1 month ago)

nice


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAmygdala
Learner

Registered: 06/30/04
Posts: 158
Last seen: 1 year, 7 months
Re: spore prints can be contaminated? [Re: orichalc]
    #3745592 - 02/07/05 03:32 PM (12 years, 1 month ago)

I second that...

NICE  :grin:

prototypicalman... no comment?  :smirk:


--------------------
"Only two things are infinite, the universe and human stupidity, and I'm not sure about the former." --Albert Einstein


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFluxburn
.
 User Gallery

Registered: 10/23/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 6 years, 4 months
Re: spore prints can be contaminated? [Re: Amygdala]
    #3777055 - 02/14/05 03:00 AM (12 years, 1 month ago)

how the hell did u make that?


--------------------
ABSTRACT ART (Mine) http://nathanbelomy.com


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVertigo6911
Entheobotanist
Male User Gallery

Folding@home Statistics
Registered: 12/04/04
Posts: 1,834
Loc: Netherlands
Last seen: 10 years, 4 months
Re: spore prints can be contaminated? [Re: Fluxburn]
    #3777129 - 02/14/05 03:31 AM (12 years, 1 month ago)

scuse me but doesent liquid culture exist specificly so u can use
dirty sporeprints?

just make a bunch of plates, some will be contammed some will be clean... no reason to trow out the print yo.


--------------------
-Know ye not that ye are gods?-
My homepage


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 7 years, 11 months
Re: spore prints can be contaminated? [Re: Vertigo6911]
    #3777136 - 02/14/05 03:35 AM (12 years, 1 month ago)

Prisoner did u make or buy that?


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCyber
Ash
Male User Gallery Arcade Champion: Yeti Sports

Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 10 months, 16 days
Re: spore prints can be contaminated? [Re: LengthyFellow]
    #3777525 - 02/14/05 08:16 AM (12 years, 1 month ago)

To clean up a contaminated print you will have to use agar and culture it out. Karo does nothing, the contamination will grow in karo just as good as the spores.

Why spend $200 on a flow hood when you can bild a glove box for $20





Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVertigo6911
Entheobotanist
Male User Gallery

Folding@home Statistics
Registered: 12/04/04
Posts: 1,834
Loc: Netherlands
Last seen: 10 years, 4 months
Re: spore prints can be contaminated? [Re: Cyber]
    #3777531 - 02/14/05 08:20 AM (12 years, 1 month ago)

nice glovebox... the thing i wonder about those is how to make sure u dont introduce contams when u place ure equiptment in the chamber...


--------------------
-Know ye not that ye are gods?-
My homepage


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCyber
Ash
Male User Gallery Arcade Champion: Yeti Sports

Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 10 months, 16 days
Re: spore prints can be contaminated? [Re: Vertigo6911]
    #3777542 - 02/14/05 08:29 AM (12 years, 1 month ago)

That would depend on how you use it.

I have had my Glove Box hooked up to a hepa filter, In those instances it is a forced air box.

If you are using it with out forced air you will load it, let it set for a while, then use your sterilizing agent (alcohol or lysol) inside the box. Then let it set for a while.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVertigo6911
Entheobotanist
Male User Gallery

Folding@home Statistics
Registered: 12/04/04
Posts: 1,834
Loc: Netherlands
Last seen: 10 years, 4 months
Re: spore prints can be contaminated? [Re: Cyber]
    #3777559 - 02/14/05 08:45 AM (12 years, 1 month ago)

kewl thxz


--------------------
-Know ye not that ye are gods?-
My homepage


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, HEPA Filter, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Spore print contamination? Stevo21 582 1 05/31/04 05:02 PM
by muse_sick
* Spore Print Contamination? YellowSubmarine 1,030 4 11/21/03 11:49 PM
by YellowSubmarine
* Utilizing unsterile spore prints Acaterpillar 1,346 8 05/11/09 04:38 PM
by caphillkid
* How do you use spore prints efficently? dnkyrhubarb 6,191 10 08/21/16 01:09 PM
by Kenetic
* First Spore Print!
( 1 2 all )
atrilis 2,502 23 10/14/08 10:45 PM
by dothedew69
* Another Way To Take a Sterile Spore Print?
( 1 2 3 all )
AdoreChampignons 13,345 45 10/01/15 05:46 PM
by BurkesGarden
* Spore printing: fresh or dry caps? livewire 2,315 4 12/06/10 07:06 PM
by kevbo
* going straight from spore printing to syringe..(in the mmgg) ismokeweed 1,438 4 09/24/10 03:59 PM
by NineInchNails

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
1,874 topic views. 9 members, 74 guests and 41 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.057 seconds spending 0.006 seconds on 14 queries.