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Amazon Shop for: ½ Pint Jars, Microscope

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Offlinejman9912
ShroomerZ
Registered: 01/09/05
Posts: 127
Last seen: 11 years, 9 months
PCing the jars.
    #3736733 - 02/05/05 08:25 PM (11 years, 9 months ago)

How long should I wait after I PC the jars to inoculate them? And when I sterilize the end of the syringe with a lighter or something. How long should I wait?


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: PCing the jars. [Re: jman9912]
    #3736767 - 02/05/05 08:31 PM (11 years, 9 months ago)

8-12 hrs at least , but I would wait till next day if was me , just to be safe. Why risk harming the spores ?
Till it glows red , then let cool 15 mins or so , squirt just a drop out to cool tip some more , then stick in jar to do inoc .
GL


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OfflineLethalX5
Male

Registered: 01/07/05
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Re: PCing the jars. [Re: jman9912]
    #3736769 - 02/05/05 08:31 PM (11 years, 9 months ago)

just let them sit overnight to be safe. even if they feel cool to the touch they could still be hot in the center. and after you flame the tip of the needle i like to press a paper towel soaked in H202 for a few sec then inoculate.


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"To get what you've never had, You must do what you've never done."


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Offlinejman9912
ShroomerZ
Registered: 01/09/05
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Re: PCing the jars. [Re: tripndicular]
    #3736771 - 02/05/05 08:31 PM (11 years, 9 months ago)

Thanks.


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OfflineSunshinePounder
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Registered: 11/19/04
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Re: PCing the jars. [Re: jman9912]
    #3736774 - 02/05/05 08:32 PM (11 years, 9 months ago)

Wait til the jars are completely cool. When you heat the needle and let it cool for a second or two, then shoot just a little solution out before inoculating.
GL


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Offlinedmc_
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Registered: 12/24/03
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Re: PCing the jars. [Re: tripndicular]
    #3737321 - 02/05/05 10:28 PM (11 years, 9 months ago)

Quote:

tripndicular said:
8-12 hrs at least , but I would wait till next day if was me , just to be safe. Why risk harming the spores ?
Till it glows red , then let cool 15 mins or so , squirt just a drop out to cool tip some more , then stick in jar to do inoc .
GL




This is alittle over cautious compared to my procedures, none the less, great advice.


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OfflineJTHM
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Registered: 01/05/05
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Re: PCing the jars. [Re: dmc_]
    #3737454 - 02/05/05 10:56 PM (11 years, 9 months ago)

wow 15 min for the needle to cool.....i wait like 5-10 seconds and my cakes all colonized


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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 11 years, 5 months
Re: PCing the jars. [Re: JTHM]
    #3738693 - 02/06/05 02:30 AM (11 years, 9 months ago)

Yeah that "15 min needle cool down" is not necessary at all. Heat it till its red, then wipe off lighter residue with a alc. soaked cotton ball. When the needle gets wet with the rubbing alc. it will instantly cool. I would think if you waited 15 mins after you flame sterilized, you got a good 14.5 mins of time for spores and what not to land on the needle.


--------------------
Don't grow mushrooms if it is illegal where you live.


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Offlinejman9912
ShroomerZ
Registered: 01/09/05
Posts: 127
Last seen: 11 years, 9 months
Re: PCing the jars. [Re: AnonymousNewbie]
    #3738782 - 02/06/05 02:36 AM (11 years, 9 months ago)

oh. i like the alcohol cotton tricker


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Offlinejonas
member

Registered: 11/19/02
Posts: 499
Last seen: 10 years, 9 months
Re: PCing the jars. [Re: dmc_]
    #3738804 - 02/06/05 02:37 AM (11 years, 9 months ago)

Quote:

dmc_ said:
Quote:

tripndicular said:
8-12 hrs at least , but I would wait till next day if was me , just to be safe. Why risk harming the spores ?
Till it glows red , then let cool 15 mins or so , squirt just a drop out to cool tip some more , then stick in jar to do inoc .
GL




This is alittle over cautious compared to my procedures, none the less, great advice.




agreed


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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 11 years, 5 months
Re: PCing the jars. [Re: jonas]
    #3738945 - 02/06/05 02:53 AM (11 years, 9 months ago)

Once again I think that the 15 min cool down is totally futile if your trying to sterilize the needle. I'm going to see how many spores will collect if any on the needle during that 15 min cool down after heating. I just got a new oil immersion microscope which should be up to the challenge. PM me if you want to know how it goes... (Note, i will heat and let the needle cool down in my bedroom - its not a dirty room at all - but its far from a perfectly sterile environment) After the 15 mins, ill put the needle under the scope and see if I can find anything interesting.


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Don't grow mushrooms if it is illegal where you live.


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Offlinejonas
member

Registered: 11/19/02
Posts: 499
Last seen: 10 years, 9 months
Re: PCing the jars. [Re: AnonymousNewbie]
    #3738954 - 02/06/05 02:54 AM (11 years, 9 months ago)

Nobody is arguing with you on that.


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OfflineAnonymousNewbie
Mad Scientist
Registered: 06/20/04
Posts: 211
Loc: England
Last seen: 11 years, 5 months
Re: PCing the jars. [Re: jonas]
    #3739003 - 02/06/05 03:08 AM (11 years, 9 months ago)

Sorry if you think I'm being rude jonas, i just saw that you agreed with dmc when he said "none the less, great advice". I think it is bad advice, and I hope to prove it with my new scope.


--------------------
Don't grow mushrooms if it is illegal where you live.


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Offlinejonas
member

Registered: 11/19/02
Posts: 499
Last seen: 10 years, 9 months
Re: PCing the jars. [Re: AnonymousNewbie]
    #3739028 - 02/06/05 03:16 AM (11 years, 9 months ago)

I was agreeing with the over-cautious part. To some degree its good advice too, I do have to disagree on the 15 minute waiting time tho. I think it definately opens up the door to contams but even still it's not making the chances of getting contams that much higher. I suppose it's better than killing the spores. But yeah, definately over-cautious, and potentially harmful.


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Amazon Shop for: ½ Pint Jars, Microscope

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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