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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Vermiculite

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Offlinedoomsdayrobert
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MULTIPLE FAILURES
    #3736359 - 02/05/05 07:11 PM (12 years, 5 months ago)

I'm new to growing and I've had some success, but the past couple times I've tried my jars won't colonize. They don't get contaminated, but nothing is happening. I'm using the spores from some that I grew earlier, so I know the spores are good... Any advise?


Edited by doomsdayrobert (02/05/05 07:16 PM)


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Invisibleagar
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Re: Multiple failures [Re: doomsdayrobert]
    #3736388 - 02/05/05 07:16 PM (12 years, 5 months ago)

May be sterile spores? Other than that, double check procedures, as somewhere in the line..... something is WRONG.


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Offlinedoomsdayrobert
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Re: Multiple failures [Re: agar]
    #3736408 - 02/05/05 07:21 PM (12 years, 5 months ago)

my spores should be contaminate-free... I'm using a heating pad, but it's not drying out the cakes. I was wondering if the brand of brown rice makes a difference, or the kind of vermiculite. The spore solution I made was pretty concentrated. I thought it was a sure bet.


Edited by doomsdayrobert (02/05/05 07:53 PM)


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Invisibletripndicular
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Re: Multiple failures [Re: doomsdayrobert]
    #3736505 - 02/05/05 07:37 PM (12 years, 5 months ago)

Quote:

doomsdayrobert said:
my spores should be sterile... I'm using a heating pad, but it's not drying out the cakes. I was wondering if the brand of brown rice makes a difference, or the kind of vermiculite. The spore solution I made was pretty concentrated. I thought it was a sure bet.


]

NO...NO he meant sterile as in not able to produce offspring(I think is what he meant) :confused:. That is kinda slim, but a possibility. Like agar says go over procedures again you missed something down the line somewheres.
GL


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Invisibleagar
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Re: Multiple failures [Re: doomsdayrobert]
    #3736515 - 02/05/05 07:39 PM (12 years, 5 months ago)

I meant, as in non-fertile, as Trip says.


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Invisibletripndicular
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Re: Multiple failures [Re: agar]
    #3736557 - 02/05/05 07:48 PM (12 years, 5 months ago)

Ya know that is worse than my recent dilema of caps that refuse to dump spores.


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinehyphae
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Re: Multiple failures [Re: doomsdayrobert]
    #3736567 - 02/05/05 07:49 PM (12 years, 5 months ago)

Are you rushing things at all? How long after PCing did you inoculate? I know thats way too obvious but thats the first question that comes to my mind. Also what your incubating temps?


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Getting the most out of your casings!, A pinning strategy.
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinedoomsdayrobert
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Re: Multiple failures [Re: hyphae]
    #3736620 - 02/05/05 07:59 PM (12 years, 5 months ago)

I'm kinda poor, so i just steam sterilize the jars. I havn't had any problems with contamination using this method. I try to get the job done as quick as possible, but i'm still cautious. I am inoculating the jars as soon as the jars cool to room temperature. I'm not exactly sure on the incubation temperature. The jars are not hot at all, but they are a little warmer than room temperature.


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Invisibletripndicular
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Re: Multiple failures [Re: doomsdayrobert]
    #3736649 - 02/05/05 08:04 PM (12 years, 5 months ago)

So... NO pc I take it ?
When did you inoc ... right after this steam method? As in how much time did you wait?
You should wait at least8-12 hrs IMO to make damn certain cool enough. If it felt warmer than room temp to your hand which is 98 those jars may have been almost 100 +degrees when you inoc'd.Not good for the spores if 104 or more.


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinedoomsdayrobert
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Re: Multiple failures [Re: tripndicular]
    #3736672 - 02/05/05 08:09 PM (12 years, 5 months ago)

After i take the jars out of the pot i use to sterilize them i let them cool for a while. When they aren't HOT i put them in the fridge for a a while until they are completely cool (about room temperature)


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Invisibletripndicular
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Re: Multiple failures [Re: doomsdayrobert]
    #3736693 - 02/05/05 08:13 PM (12 years, 5 months ago)

Like I said I would let them cool 8-12 hrs before attempting to inoc. at least.
On the lighterside... maybe you should change you ident...doomsday...mushies no like doomsday :wink:


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinehyphae
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Re: Multiple failures [Re: tripndicular]
    #3737544 - 02/05/05 11:11 PM (12 years, 5 months ago)

doomsdayrobert if jars feel warm at all your setting yourself up for failure trust me. Patience is a virtue remember? :wink: Again if jars feel warm at all then the core temps will be much higher this you can't feel but trust us. Those jars should be cool enough after a few hours depending on how, where they are situated. We've not talking hot we're talking warm here BTW :wink: Also mycelium thermodeath limits are about 106F. spores can handle higher temps 122F. (50C.) GL guys


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinedoomsdayrobert
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Re: Multiple failures [Re: hyphae]
    #3820239 - 02/23/05 01:11 AM (12 years, 5 months ago)

I just got a thermometer for the incubation, and it reads 78-80 degrees
ferenheit where the jars are stored. Is this suitable, or do they need to be any warmer?


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Invisiblekorins
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Re: Multiple failures [Re: doomsdayrobert]
    #3820641 - 02/23/05 02:44 AM (12 years, 5 months ago)

78-80F will do, but the optimal incubating temperature range is said to be 82-86F. If you keep it stable at 78-80F, the mycelium colonization will be a little slower, that's all. Also, it's important to keep your temperatures STABLE as well as you can so as to avoid stunting the growth. Good luck man!  :thumbup:


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Offlinedoomsdayrobert
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Re: Multiple failures [Re: korins]
    #4060628 - 04/15/05 11:02 PM (12 years, 3 months ago)

thank you for actually reading this. i've still tried and have had no colonization at all. i need to just start over with new spores :frown: :beer: :cig: spores are impossible to come across in wyoming. i would just order some online, but i don't have a credit card.


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OfflineHippieChick
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Re: Multiple failures [Re: doomsdayrobert]
    #4060826 - 04/16/05 12:25 AM (12 years, 3 months ago)

You don't have to have a credit card. Some sponsors will take money orders, just takes a few day's longer to get your spores. And definitely let your jars cool long enough.
Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Offlineclosetgrower
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Re: Multiple failures [Re: HippieChick]
    #4060868 - 04/16/05 12:34 AM (12 years, 3 months ago)

try making honey or karo water(find a tek)then put your spores in it to see if they grow. if they grow, use them to inoc. your jars and growth will start right away.


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Into this house we're born, into this world we're thrown.


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Offlinegratefulredhead
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Re: Multiple failures [Re: tripndicular]
    #4061505 - 04/16/05 03:54 AM (12 years, 3 months ago)

tripndicular--where did you get that extraordinary photo?? impressively beautiful...


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Invisibledeathbychimney
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Registered: 02/26/05
Posts: 239
Re: Multiple failures [Re: gratefulredhead]
    #4061522 - 04/16/05 04:06 AM (12 years, 3 months ago)

order new spores by M.O. like hippiechick said


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Offlinehawksapprentice
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Re: Multiple failures [Re: deathbychimney]
    #4061588 - 04/16/05 04:43 AM (12 years, 3 months ago)

PM me if you would rather have a free print.


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"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Vermiculite

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