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OfflineHillman
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Registered: 06/29/01
Posts: 4
Last seen: 12 years, 11 months
monokariotic culture
    #372625 - 08/15/01 11:04 PM (17 years, 1 month ago)

Hi.
I had a syringe with very weak spores(i supposed they died from the heat in the plane/truck in the mail) . My first batch all died from contamination because i didn't sterilized my jar/brf/etc good enough. Anyways, i started over and i got ONE spore to grow in one of my jar. So now the jar is almost all colonized but i read that the mycelium has to breed(or something) with another spore to form dikaryote. And if the mycelium formed a dikaryote then it will fruit. Did i understood well?


Also, I put some spores in an honey-water culture thingy and ONE spore worked and produced a massive blob that i broke several times(by shaking it). And i took some uncolonized jars(nothing in it) and injected some mycelium(like 10 cc for each jar) from the blob and after like 5 days these jars are almost fully colonized. So my question is will these jars fruit/pin or i'll just have a beautiful mycelium factory?


Thanks alot!



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OfflineShiznitz
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Registered: 04/19/01
Posts: 623
Loc: Canada
Last seen: 16 years, 9 months
Re: monokariotic culture [Re: Hillman]
    #372631 - 08/15/01 11:10 PM (17 years, 1 month ago)

They should pin, but if they don't eat the mycelium. You can trip off of that.

LEGALIZE...


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LEGALIZE...


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Offlineshellacct
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Registered: 02/02/01
Posts: 427
Loc: BAD DOG!
Last seen: 13 years, 4 months
Re: monokariotic culture [Re: Hillman]
    #373149 - 08/16/01 05:48 PM (17 years, 1 month ago)

how the hell did u isolate a single spore.... tahts as hard as fuck to do w/o REAL lab equipment and know how.... i mea it may LOOK like it was just one spore, but that little clumo was more likely ONE-THOUSAND

"Trying is the first step towards failure" -Homer J. Simpson

In the above, Any and all references to reality are entirely fictional.

Just because you are paranoid, don't mean they're not after you...


--------------------
"Trying is the first step towards failure" -Homer J. Simpson

In the above, Any and all references to reality are entirely fictional.


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Malt Extract

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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