monokariotic culture - Mushroom Cultivation

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Hillman
Stranger

Registered: 06/29/01
Posts: 4
Last seen: 19 years, 2 months

monokariotic culture

#372625 - 08/15/01 09:04 PM (23 years, 3 months ago)

Hi.
I had a syringe with very weak spores(i supposed they died from the heat in the plane/truck in the mail) . My first batch all died from contamination because i didn't sterilized my jar/brf/etc good enough. Anyways, i started over and i got ONE spore to grow in one of my jar. So now the jar is almost all colonized but i read that the mycelium has to breed(or something) with another spore to form dikaryote. And if the mycelium formed a dikaryote then it will fruit. Did i understood well?

Also, I put some spores in an honey-water culture thingy and ONE spore worked and produced a massive blob that i broke several times(by shaking it). And i took some uncolonized jars(nothing in it) and injected some mycelium(like 10 cc for each jar) from the blob and after like 5 days these jars are almost fully colonized. So my question is will these jars fruit/pin or i'll just have a beautiful mycelium factory?

Thanks alot!

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Shiznitz
addict

Registered: 04/19/01
Posts: 623
Loc: Canada
Last seen: 23 years, 17 days

Re: monokariotic culture

[Re: Hillman]
#372631 - 08/15/01 09:10 PM (23 years, 3 months ago)

They should pin, but if they don't eat the mycelium. You can trip off of that.

LEGALIZE...

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LEGALIZE...

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shellacct
addict

Registered: 02/01/01
Posts: 427
Loc: BAD DOG!
Last seen: 1 year, 29 days

Re: monokariotic culture

[Re: Hillman]
#373149 - 08/16/01 03:48 PM (23 years, 3 months ago)

how the hell did u isolate a single spore.... tahts as hard as fuck to do w/o REAL lab equipment and know how.... i mea it may LOOK like it was just one spore, but that little clumo was more likely ONE-THOUSAND

"Trying is the first step towards failure" -Homer J. Simpson

In the above, Any and all references to reality are entirely fictional.

Just because you are paranoid, don't mean they're not after you...

--------------------
"Trying is the first step towards failure" -Homer J. Simpson

In the above, Any and all references to reality are entirely fictional.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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