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OfflineNumba9
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Big-time contam problem.Pleeeez help
    #372465 - 08/15/01 06:22 PM (15 years, 3 months ago)

I just innoculated 9 jars and only got 2 without any contams,, i use an alcohol flame but can`t seem to get the needle red-hot all the way. My question is would butane flame work ? ..like from a pencil torch. not a cigarette lighter.

?One side will make you grow taller, and the other side will make you grow shorter" said the caterpillar "One side of what"? said Alice "Of the mushroom" as he crawled off


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Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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Offlineuniboner
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372471 - 08/15/01 06:26 PM (15 years, 3 months ago)

umm.. my needle never gets red hot, and I have not had contam problems like that.. you sure the needle is the source for the contams? How did you sterilize the substrate? and did you get the spore syringe from a reliable source?

peace,
uniboner

Without nipples, breasts would be pointless...


--------------------
sleep is for the weak.


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OfflineNumba9
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Re: Big-time contam problem.Pleeeez help [Re: uniboner]
    #372474 - 08/15/01 06:29 PM (15 years, 3 months ago)

Sterilized for 1 full hour at 15 lbs starting the timer after the weight starts hissing....seems the contams are exactly at the point of entry of the needle directly below the taped holes.This is why i asked about getting the needle hotter.. My resources for syringes are PF and sporeworks. never had this much problem with contams before

?One side will make you grow taller, and the other side will make you grow shorter" said the caterpillar "One side of what"? said Alice "Of the mushroom" as he crawled off


--------------------
Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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OfflineShiznitz
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372475 - 08/15/01 06:29 PM (15 years, 3 months ago)

Your contam didn't neccissarily come from the needle. You don't have to get it red hot either, just a quick 3 second flame will do. Since you did flame it good, I doubt the syringe is the problem. Unless you only flamed the needle once? Flame it right before each jar, better yet, each hole.

Did you pressure cook the jars? Or boil them real good?
Make sure you have a real clean environment. Read some "sterile tips" at The Hawks Eye

LEGALIZE...


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LEGALIZE...


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OfflineNumba9
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Re: Big-time contam problem.Pleeeez help [Re: Shiznitz]
    #372477 - 08/15/01 06:31 PM (15 years, 3 months ago)

I did heat before each hole..... would cooling the needle in lysol maybe help?

?One side will make you grow taller, and the other side will make you grow shorter" said the caterpillar "One side of what"? said Alice "Of the mushroom" as he crawled off


--------------------
Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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Invisiblepuscle
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372479 - 08/15/01 06:35 PM (15 years, 3 months ago)

Verm barrier?



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Invisiblepuscle
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372481 - 08/15/01 06:37 PM (15 years, 3 months ago)

By the way, what color contam are we fighting?



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OfflineNumba9
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Re: Big-time contam problem.Pleeeez help [Re: puscle]
    #372482 - 08/15/01 06:38 PM (15 years, 3 months ago)

verm barrier bro yup....... wipe the inner edge of jar clean then add dry verm . about a half inch.... really puzzledn why i get green mold right at the needle point of entry

?One side will make you grow taller, and the other side will make you grow shorter" said the caterpillar "One side of what"? said Alice "Of the mushroom" as he crawled off


--------------------
Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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Invisiblepuscle
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372491 - 08/15/01 06:53 PM (15 years, 3 months ago)

That's a tough call. Is there anything you've changed since previous, successful crops? I tend to doubt your needle flaming technique as being the problem. I sterilize my syringes simply by setting my syringe in a beaker partially filled with alchohol.



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Invisibletriptor
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372492 - 08/15/01 06:54 PM (15 years, 3 months ago)

Shit Happens

Huh? Who just said that? Wow man!


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Zippity freakin doo dah


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Offlinesporedroppings
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372500 - 08/15/01 07:11 PM (15 years, 3 months ago)

i'm new here, but could i suggest the oven tek if you're not already using it?



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OfflineNumba9
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Re: Big-time contam problem.Pleeeez help [Re: triptor]
    #372511 - 08/15/01 07:47 PM (15 years, 3 months ago)

So what you`re saying Triptor is if i didn`t have to read your "Shit" posts... It would`nt happen? screw that kind of "shit" answer..... This forum is for informative and helpful answers in the shroom community...... not smart ass remarks

?One side will make you grow taller, and the other side will make you grow shorter" said the caterpillar "One side of what"? said Alice "Of the mushroom" as he crawled off


--------------------
Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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OfflineL3D
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Re: Big-time contam problem.Pleeeez help [Re: puscle]
    #372527 - 08/15/01 08:20 PM (15 years, 3 months ago)

I do the same thing and just flame it on first use and last before putting it away. then put in alcohol between holes/jars.

L3D



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OfflineSuntzu
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Re: Big-time contam problem.Pleeeez help [Re: L3D]
    #372538 - 08/15/01 08:56 PM (15 years, 3 months ago)

just to figure out where the contamination is coming from, you could do some negative controls. . .with PF jars it's easy since you can make them so easily.

Sterilize everything as normal.
Keep one jar aside, don't inject anything, but incubate with the others.
Take another jar and stick the needle in each hole, but don't inject anything [dry inoc.] Incubate as usual.

Inject the others from the same pressure cooker batch normally, and you should have an idea of what's going on. If you REALLY want to go all the way, do two or three of each negative control. If they don't contaminate, you can always use them later.
I'd also agree that it's probably not the needle itself giving you problems.

I only eat veils


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OfflineShadowTH
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372539 - 08/15/01 08:57 PM (15 years, 3 months ago)

'Eya =)
I'm completely new here, and haven't had too much experience yet, just a LOT of reading and a few jars currently colonizing. In fact, this's my first post, so.. don't take anything I say too heavily..
Your situation is pretty confusing.. seems like you're doing everything right, but there're a few things that come to mind if you haven't found the answer.. Have you tried cleaning the whole syringe really well? When you put the tape on, are your hands clean, or do you at least not touch the part exposed to the inside of the jar? Are the holes clean-cut so nothing can hide in the edges? How long do you keep the tape off when you Innoculate? Another thing, though kinda stupid sounding.. do you ever remember possibly sucking air into the syringe or anything that might get contamination stuck in the needle?

If you've checked all of those off, I really can't suggest anything other than an innoculating hood, a LOT of lysol, and wiping the needle with alcohol too..

I know most of that is just logic, but I hope some part of that was helpful

-Mark



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Offlinefredhedd
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #372559 - 08/15/01 09:44 PM (15 years, 3 months ago)

i'll try to help as much as i can.. you said you innoculated nine jars and got two w/out contam.. i think i know why. nine plus two is eleven.. i have a little brother who is eleven... when he doesnt do what he is supposed to .. we keep him in his room till he is better.. so i would suggest you put them in the corner or something till they do what they are supposed to .. if that doesnt work try to put pepper on their tongues. glad i could help.

i'm fred
kill yourself


--------------------
i'm fred
kill yourself


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OfflineShiznitz
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Re: Big-time contam problem.Pleeeez help [Re: fredhedd]
    #372568 - 08/15/01 09:53 PM (15 years, 3 months ago)

Ok fredd, I've had enough of your shit. Shut the fuk up NOW.

LEGALIZE...


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LEGALIZE...


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Offlinetrollhunter
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Re: Big-time contam problem.Pleeeez help [Re: Shiznitz]
    #372575 - 08/15/01 10:10 PM (15 years, 3 months ago)

just a idea ,but when you are done pcing your jars are they on tight with the tape on them .because if that is the case when you go to put the neddle in there is a vacum in the jar might be sucking in the contam that way.you never know i just thought i would try to help,hope you get it worked out :-)
rats and trolls are a dieing breed



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OfflineL3D
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Re: Big-time contam problem.Pleeeez help [Re: Shiznitz]
    #372632 - 08/15/01 11:10 PM (15 years, 3 months ago)

I agree Fred has pissed me off in almost every message I have just read. this is not funny anymore(Never was to begin with).

L3D



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Offlineshellacct
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Re: Big-time contam problem.Pleeeez help [Re: Numba9]
    #373148 - 08/16/01 05:46 PM (15 years, 3 months ago)

you should review your sterile teknique..... there are more likely flaws there than anywhere else.



"Trying is the first step towards failure" -Homer J. Simpson

In the above, Any and all references to reality are entirely fictional.

Just because you are paranoid, don't mean they're not after you...


--------------------
"Trying is the first step towards failure" -Homer J. Simpson

In the above, Any and all references to reality are entirely fictional.


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