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Offlineo0brandnewcolony
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No growth?
    #3721675 - 02/02/05 11:20 PM (11 years, 10 months ago)

Hello all. I have inoculated into a sterile vermiculite/brown rice substrate. I inoculated about 4-5 days ago and stored them in a shut off mini fridge. Still no signs what so ever. After I boiled the joars to steralize them I waited about 5 days for the syringes, at which time I inoculated. What could I have done wrong?

Edit: I am using the b+ strain.


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Edited by o0brandnewcolony (02/02/05 11:25 PM)


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Offlinetrippinpimp
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Re: No growth? [Re: o0brandnewcolony]
    #3721703 - 02/02/05 11:24 PM (11 years, 10 months ago)

if its to cold it will slow the growth i think the idle temp is like 84

hope it gos well


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InvisibleMrMaddHatter
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Re: No growth? [Re: o0brandnewcolony]
    #3721706 - 02/02/05 11:25 PM (11 years, 10 months ago)

Are you sure the jars haven't contaminated? Open one up and see if it smells sour.
Then again, if you inoculated form spore syringe, it could take more time to show signs of growth. Some times 7-10 days.


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Offlineo0brandnewcolony
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Re: No growth? [Re: MrMaddHatter]
    #3721717 - 02/02/05 11:27 PM (11 years, 10 months ago)

It's about 75 degrees in there, it has a heat function. Should I run the heat thing for awile then measure the temp and shut it off and let it sit since its insulated? I will smell them soon.


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Invisibledog
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Re: No growth? [Re: o0brandnewcolony]
    #3721731 - 02/02/05 11:29 PM (11 years, 10 months ago)

You waited too long to inoculate but that fact alone should not hinder germination, unless maybe a fast growing contam took hold. It's best to inoculate as soon as the jars are cool.

What at what temperature are you incubating? At even the ideal temperature of 86F they can take up to a week to show growth. At cooler temperatures that time is lengthened.

There are other things that could be wrong but this is all I can offer with the information you provided.


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Offlineo0brandnewcolony
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Re: No growth? [Re: dog]
    #3721737 - 02/02/05 11:31 PM (11 years, 10 months ago)

What else can I tell you?


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InvisibleMrMaddHatter
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Re: No growth? [Re: o0brandnewcolony]
    #3721748 - 02/02/05 11:32 PM (11 years, 10 months ago)

75f is a little low. Try somehow to get it up to 80-85 like dog said.


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Offlineo0brandnewcolony
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Re: No growth? [Re: o0brandnewcolony]
    #3721752 - 02/02/05 11:32 PM (11 years, 10 months ago)

It doesn't smell sour at all, just like birdseed or something.


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Offlineo0brandnewcolony
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Re: No growth? [Re: o0brandnewcolony]
    #3721761 - 02/02/05 11:33 PM (11 years, 10 months ago)

Where's should I place them to achieve a temperature like that?


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Invisibledog
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Re: No growth? [Re: o0brandnewcolony]
    #3721766 - 02/02/05 11:34 PM (11 years, 10 months ago)

The ratios of verm/water/BRF and how you mixed them, and your inoculation method. Was it a Shroomery vendor syringe?


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Re: No growth? [Re: o0brandnewcolony]
    #3721773 - 02/02/05 11:35 PM (11 years, 10 months ago)

Then its probably not contaminated (probably). Give it atleast 10 days before throwing them out. Patientce is key.


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Offlineo0brandnewcolony
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Re: No growth? [Re: dog]
    #3721775 - 02/02/05 11:35 PM (11 years, 10 months ago)

I Got the syringe from thehawkseye.com

I also use the guide in the basic cultivation section, the one with the calculater. I inoculated 2 jars. And I still have enough supplied and spores for 20 more.


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OfflineSunshinePounder
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Re: No growth? [Re: o0brandnewcolony]
    #3721782 - 02/02/05 11:36 PM (11 years, 10 months ago)

I say you are fine. But you need a thermometer.
I did exactly as you. One strain took off the other lagged, but it took off eventually.
How are you heating the freezer, I do it this way too

GL


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Offlineo0brandnewcolony
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Re: No growth? [Re: SunshinePounder]
    #3721803 - 02/02/05 11:38 PM (11 years, 10 months ago)

There is a termometer stuck in there, it reads 76. It is a mini-fridge that has a cool and heat function, how hot can I let the jars get?


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Invisibledog
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Re: No growth? [Re: o0brandnewcolony]
    #3721855 - 02/02/05 11:43 PM (11 years, 10 months ago)

The most common mistake newbs make with the PF tek is water content. Anno posted an almost foolproof way to do it at  Fungi Fun. If your jar's moisture content looks right and there are no funny smells then just wait it out. This is not a hobby for the impatient.  :wink:


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Re: No growth? [Re: dog]
    #3721897 - 02/02/05 11:49 PM (11 years, 10 months ago)

Quote:

dog said:
This is not a hobby for the impatient.  :wink:





Damn straight. :thumbup:


--------------------
I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.
Hunter S. Thompson

It's always tea-time, and we've no time to wash the things between whiles.

Don't be a fool, this is a war on drugs. Act like. The government sure does!


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Offlineo0brandnewcolony
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Re: No growth? [Re: dog]
    #3721900 - 02/02/05 11:49 PM (11 years, 10 months ago)

What's the hottest temperature to let the jars sit in?


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OfflineSunshinePounder
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Re: No growth? [Re: o0brandnewcolony]
    #3721942 - 02/02/05 11:53 PM (11 years, 10 months ago)

82-85 F is ideal. 106 F is max


--------------------
My [url=www.http://tinyurl.com/6valr target=_blank]Unitarian Jihad Name[/url] is: Sister Claymore of The Short Path
Get yours

If I was the sun, I'd look for the shade~Liberty

Baby I love you, and you love me. Let's love each other to eternity~


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Offlineo0brandnewcolony
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Re: No growth? [Re: SunshinePounder]
    #3721966 - 02/02/05 11:56 PM (11 years, 10 months ago)

So could I simply run my heat function on my mini-fridge? I guess I should run it to see how hot it gets first. If it gets too hot, I will build an incubator with a water heater and 2 boxes. Is 106 so hot that its like having them too cold? Or will it still produce good mycellium?


--------------------


LSA Extraction Pictorial



Keeping my spirit alive.


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Invisibledog
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Re: No growth? [Re: SunshinePounder]
    #3721975 - 02/02/05 11:57 PM (11 years, 10 months ago)

Quote:

82-85 F is ideal. 106 F is max




106F = dead mycelium. Don't exceed 86F.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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