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Offlinefirsttimer101
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Registered: 01/23/05
Posts: 279
Last seen: 5 years, 8 months
Agar not solidifying!
    #3716283 - 02/01/05 11:56 PM (11 years, 10 months ago)

Hey guys, I've got an annoying problem with agar preparation. I poured 500 ml of water into a pan and added 2 tsp (10 grams) of agar. The agar dissolved in the boiling water, then I poured the plates. The agar never solidified. This is the second time this has happened and it's starting to piss me off, especially because I pay around 25 dollars canadian for a measly 40 grams of PDA at a science supply store. I am 100% sure I got the proportions right and don't know what's going on. The instructions in both my mycology books say the typical proportion is 1000 ml of water to 20 grams of agar. Is this proportion off? Thanks for the help guys.


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InvisibleATWAR
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Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: Agar not solidifying! [Re: firsttimer101]
    #3716484 - 02/02/05 12:31 AM (11 years, 10 months ago)

Quote:

firsttimer101 said:
I poured 500 ml of water into a pan and added 2 tsp (10 grams) of agar.




Is that 2 tea spoons?
If so, that is far too little.
My agar is flake style. For 150ml I use 3 grams of agar, and this is about 2 tablespoons (heaping - but estimated, I use a scale and it is a pretty big pile - And this is just the agar, nothing else). In any case, if it does not solidify, you did not add enough agar...


Edit:
Agar can degrade, so I hear. If it is a premix, upping the concentration may have negative impacts if you get too high. Large concentrations of sugar can be detrimental to growth...


--------------------
To give is to live...



Edited by ATWAR (02/02/05 12:34 AM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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